LOCUS Exported 11297 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentivirus production plasmid encoding for Archon2 fluorescent voltage reporter. ACCESSION . VERSION . KEYWORDS pHR-EF1a-Archon2-KGC-EGFP-ER2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11297) AUTHORS Piatkevich KD, Jung EE, Straub C, Linghu C, Park D, Suk HJ, Hochbaum DR, Goodwin D, Pnevmatikakis E, Pak N, Kawashima T, Yang CT, Rhoades JL, Shemesh O, Asano S, Yoon YG, Freifeld L, Saulnier JL, Riegler C, Engert F, Hughes T, Drobizhev M, Szabo B, Ahrens MB, Flavell SW, Sabatini BL, Boyden ES TITLE A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters. JOURNAL Nat Chem Biol. 2018 Feb 26. pii: 10.1038/s41589-018-0004-9. doi: 10.1038/s41589-018-0004-9. PUBMED 29483642 REFERENCE 2 (bases 1 to 11297) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11297 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(1..17) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2..18) /label=pBluescriptKS /note="For pBluescript vector" LTR 108..341 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" promoter complement(366..384) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(367..384) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(674..693) /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" primer_bind complement(907..926) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 1026..1048 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(1086..1104) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 1172..1276 /gene="bla" /label=AmpR promoter CDS 1277..2137 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1495..1514) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 2308..2896 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2797..2816 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3050..3067 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(3137..3157) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 3142..3471 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 3322..3457 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3384..3403 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" intron 4605..4670 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 4800..4820 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 5245..5379 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5282..5301) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 5336..5355 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 5548..6181 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 6228..6353 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 6850..7082 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 7267..7311 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 7596..7713 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 7779..8957 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 8010..8948 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 8905..8925 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" primer_bind 8974..8993 /label=T7 /note="T7 promoter, forward primer" promoter 8974..8992 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 9037..9046 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 9910..10623 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" primer_bind complement(9952..9973) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(10213..10232) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 10560..10581 /label=EGFP-C /note="EGFP, forward primer" misc_feature 10707..11295 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(10760..10780) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(11178..11189) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" ORIGIN 1 cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac caatgactta 61 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat 121 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca 181 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 241 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 301 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg 361 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg 421 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag 481 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc 541 tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc 601 atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt 661 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc 721 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg 781 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac 841 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac 901 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc 961 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac 1021 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg 1081 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta 1141 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 1201 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 1261 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc 1321 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga 1381 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct 1441 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg 1501 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta 1561 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat 1621 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt 1681 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga 1741 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga 1801 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga 1861 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 1921 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 1981 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 2041 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 2101 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 2161 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 2221 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 2281 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 2341 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 2401 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtctttct 2461 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 2521 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 2581 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 2641 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 2701 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 2761 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 2821 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 2881 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 2941 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 3001 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt 3061 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat 3121 tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 3181 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 3241 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 3301 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 3361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 3421 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgga 3481 cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt cagggagagg 3541 cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta 3601 taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg 3661 gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg 3721 taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg 3781 tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc 3841 gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca 3901 gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg 3961 gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct 4021 ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg gattgaacag 4081 ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc 4141 ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag 4201 tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg 4261 ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc 4321 tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct 4381 gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt 4441 gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg 4501 cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg 4561 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt 4621 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct 4681 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct 4741 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc 4801 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt 4861 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg 4921 aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta 4981 ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt 5041 ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg 5101 ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca 5161 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat 5221 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa 5281 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 5341 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac 5401 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctagtggtt 5461 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg 5521 ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag 5581 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca 5641 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag 5701 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg 5761 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc 5821 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat 5881 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg 5941 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact 6001 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 6061 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 6121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 6181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca 6241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc 6301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta 6361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa 6421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc 6481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc 6541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg 6601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc 6661 aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg 6721 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga 6781 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc 6841 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc 6901 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca 6961 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg 7021 catcaagcag ctccaggcaa gaatcctggc tgtgaaagat acctaaagga tcaacagctc 7081 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct 7141 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac 7201 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag 7261 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg 7321 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg 7381 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat 7441 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga 7501 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct 7561 cgacggtatc gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg 7621 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa 7681 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat 7741 ccagtttgga tcgataagct tgatatcgaa ttccgtgagg ctccggtgcc cgtcagtggg 7801 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg 7861 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc 7921 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt 7981 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg 8041 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg 8101 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt 8161 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg 8221 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt 8281 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg 8341 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg 8401 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac 8461 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg 8521 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc 8581 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg 8641 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg 8701 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta 8761 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg 8821 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt 8881 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc 8941 catttcaggt gtcgtgagga attagcttgg tactaatacg actcactata gggagaccca 9001 agctggctag gtaagcttgg taccgagctc ggatccgcca ccatggtttc tatcgcactg 9061 caggctggtt atgacctcct cggagatggt agacctgaaa ccctgtggct cggaattggt 9121 acactgctga tgctgatcgg aaccttctac ttcctggtta gagggtgggg agtgactgac 9181 aaggatgcac gggagtacta cgcagtgccc atcctggtca gcggtattgc cagtgcagct 9241 tatctgtcaa tgttctttgg tatcggcctc acagaggtgc cagtcggtgg agagatgctg 9301 gacatctact acgcaagata cgcacactgg ctgttcagca caccactgct gctgctggac 9361 ctggcactcc tggccaaagt tgatagagtg atcatcggca ctctcgttgg cgtcgatgct 9421 ctgatgattg tcacaggcct gataggagca ctgtcacata ctgccattgc cagatactct 9481 tggtggctgt tctcaactat ctgtatgatc gtcgttctgt atgtcctggc cacttctctg 9541 cggtcagccg ctaaggagag aggcccagag gttgcctcaa ccttcaacat cctcaccgca 9601 ctggttctgg tcctgtggac tgcttaccct atcatctgga ttatcggcac cgagggtgcc 9661 ggagttgtgg gcctgggaat cgaaactctg ctgttcatgg tgctggacgt cacctgcaag 9721 gtgggcttcg ggtttatcct gctcaggtca agagccattc tgggagatac agaagcacct 9781 gaaccttcag ctggagctga taccggtgcc gccgaccgcc cggtagtagc agtgagcaag 9841 gcggccgcca agagcaggat caccagcgag ggcgagtaca tccccctgga ccagatcgac 9901 atcaacgtgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag 9961 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc 10021 acctacggca agctgaccct gaagttcatt tgcaccaccg gcaagctgcc cgtgccctgg 10081 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac 10141 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc 10201 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac 10261 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg 10321 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag 10381 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag 10441 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac 10501 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac 10561 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac 10621 aagttctgct acgagaacga ggtgtaagcg gccgcgactc tagagtcgac ctgcaggcat 10681 gcaagcttga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat 10741 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 10801 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 10861 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 10921 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 10981 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 11041 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 11101 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 11161 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 11221 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 11281 gggccgcctc cccgcat //