LOCUS       Exported                7744 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses N-terminus to aa468 of Bxb1 integrase fused to GID1 CID.
ACCESSION   .
VERSION     .
KEYWORDS    pBW2121_pCAG-PV1-Bxb1-N468-L1-GID1-NLS-BGHpA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7744)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J 
            , Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice
  JOURNAL   Nature Communications (2019) volume 10, Article number: 4845
REFERENCE   2  (bases 1 to 7744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7744
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(22..38)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(22..38)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(35..57)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    46..62
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(70..100)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    115..136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      221..355
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     282..301
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    375..509
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(412..431)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     466..485
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(577..594)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(748..1336)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(828..847)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1507..2367)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2130..2149
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2368..2472)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        2503..2882
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2884..3159
                     /label=chicken beta-actin promoter
     intron          3160..4175
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4099..4121
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4183..4202
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             6791..6811
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6821..7045
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7285..7304)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7350..7405
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7404..7423)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
       61 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
      121 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
      181 gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      241 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
      301 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
      361 ttttgcaaaa agctaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
      421 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
      481 tcatcaatgt atcttatcat gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg
      541 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
      601 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      661 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
      721 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
      781 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      841 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      901 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
      961 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1021 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1081 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1141 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1201 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     1261 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1321 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1381 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     1441 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     1501 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     1561 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     1621 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     1681 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     1741 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     1801 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     1861 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     1921 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     1981 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     2041 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     2101 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     2161 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     2221 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     2281 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     2341 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     2401 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     2461 aggggttccg cgcacatttc cccgaaaagt gccacctggg tcgacattga ttattgacta
     2521 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     2581 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     2641 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     2701 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     2761 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     2821 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     2881 tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc
     2941 caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg
     3001 gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggaaaggt
     3061 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
     3121 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gttgccttcg
     3181 ccccgtgccc cgctccgcgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
     3241 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
     3301 taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc ttaaagggct ccgggagggc
     3361 cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
     3421 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
     3481 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
     3541 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
     3601 tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc ccctccccga gttgctgagc
     3661 acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg
     3721 gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg
     3781 gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
     3841 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
     3901 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga
     3961 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc
     4021 gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg
     4081 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg
     4141 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg
     4201 tgctgtctca tcattttggc aaagaattga ttaattcgag cgaaggcgcg cccattactc
     4261 gcatccattc tcaggctgtc tcgtctcgtc tcgcacgcgt gccgccacca tgcgagccct
     4321 ggtggtcatt cgcctgagca gagtcacaga cgctactaca agccctgagc ggcagctgga
     4381 gtcctgtcag cagctgtgcg cacagcgagg atgggatgtg gtcggagtgg cagaggatct
     4441 ggacgtgagc ggggctgtcg atccattcga ccgaaagcgg agacccaacc tggcacgatg
     4501 gctggctttc gaggaacagc cctttgatgt gatcgtcgcc tacagagtgg acaggctgac
     4561 acgctcaatt cgacatctgc agcagctggt gcattgggcc gaggatcaca agaaactggt
     4621 ggtcagcgca actgaagccc acttcgacac cacaactcct tttgccgctg tggtcatcgc
     4681 actgatgggc accgtggccc agatggagct ggaagctatc aaggagcgaa accggagcgc
     4741 agcccatttc aatattcggg ccgggaaata cagaggcagc ctgccccctt ggggctatct
     4801 gcctacccgg gtggatgggg agtggagact ggtgccagac cccgtccaga gagagaggat
     4861 tctggaagtg taccacagag tggtggacaa ccacgaacca ctgcatctgg tggcccacga
     4921 tctgaatagg cgcggagtcc tgtctccaaa ggactatttt gctcagctgc agggaaggga
     4981 gccacaggga cgagaatgga gtgctaccgc actgaagcgg tctatgatca gtgaggctat
     5041 gctgggctat gcaactctga atgggaaaac cgtgagagac gatgacggag caccactggt
     5101 gcgggctgag cctattctga caagagagca gctggaagct ctgagggcag aactggtgaa
     5161 aaccagtagg gccaagcctg ctgtgtcaac accaagcctg ctgctgcgag tgctgttctg
     5221 cgcagtctgt ggcgagccag catacaaatt tgccggcggg ggaaggaagc atccccgcta
     5281 tcgatgccgg agcatggggt tccctaagca ctgtggaaac ggcactgtgg ctatggccga
     5341 atgggacgcc ttttgtgagg aacaggtgct ggatctgctg ggggacgcag agcgcctgga
     5401 aaaagtgtgg gtcgctggaa gcgattccgc tgtggagctg gcagaagtca atgccgagct
     5461 ggtggacctg acctccctga tcggatctcc tgcatacagg gcaggctccc cacagcgaga
     5521 agctctggat gcacgaattg ctgcactggc agctcgacag gaggaactgg aggggctgga
     5581 agccagaccc tctggatggg agtggcgaga aacaggccag cggtttgggg attggtggag
     5641 ggagcaggac acagcagcca agaacacttg gctgagatcc atgaatgtca ggctgacttt
     5701 cgacgtgcga ggatccggag ggtctggctc cggatcaagt ggtggcagcg gtaccatggc
     5761 cgccagcgac gaagtgaacc tgatcgagag cagaaccgtg gtgcccctga acacctgggt
     5821 gctgatctcc aacttcaagg tggcctacaa catcctgcgg aggcccgacg gcaccttcaa
     5881 cagacacctg gccgagtacc tggaccggaa agtgaccgcc aacgccaacc ctgtggacgg
     5941 cgtgttcagc ttcgacgtgc tgatcgaccg gcggatcaac ctgctgagcc gggtgtacag
     6001 acccgcctac gccgatcagg aacagccccc ctctatcctg gatctggaaa agcccgtgga
     6061 tggcgacatc gtgcccgtga tcctgttctt ccacggcggc agctttgccc acagcagcgc
     6121 caatagcgcc atctacgaca ccctgtgcag acggctcgtg ggcctgtgca aatgcgtggt
     6181 ggtgtccgtg aactaccgca gagcccccga gaacccttac ccctgcgcct acgatgatgg
     6241 ctggatcgcc ctgaactggg tcaacagcag aagctggctg aagtccaaga aagacagcaa
     6301 ggtgcacatc tttctggccg gcgatagcag cggcggcaat atcgcccata acgtggccct
     6361 gagagccggc gagtctggca tcgatgtgct gggcaatatc ctgctgaacc ccatgttcgg
     6421 cggcaacgag cggaccgaga gcgagaagtc tctggacggc aagtacttcg tgaccgtgcg
     6481 ggaccgggac tggtactgga aggcctttct gcccgagggc gaggacagag agcaccccgc
     6541 ctgcaatccc ttcagcccca gaggcaaaag cctggaaggc gtgtccttcc caaagtccct
     6601 ggtggtggtg gccggcctgg acctgatcag agattggcag ctggcctatg ccgagggcct
     6661 gaagaaagcc ggccaggaag tgaagctgat gcacctggaa aaggccaccg tgggcttcta
     6721 cctgctgccc aacaacaacc acttccacaa cgtgatggac gagatcagcg ccttcgtgaa
     6781 cgccgagtgc cccaagaaaa agcggaaggt gtgagaattc ctgtgccttc tagttgccag
     6841 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     6901 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     6961 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     7021 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     7081 gggtatcccc acgcgccctg tagcgtgtac cgctgggagt tcgtagacgg aaacaaacgc
     7141 agaatccaag cgcggccgcc caggatacat agattaccac aactccgagc ccttccaccg
     7201 ctagcgcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc
     7261 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat
     7321 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     7381 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
     7441 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat
     7501 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
     7561 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
     7621 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
     7681 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct
     7741 gcag
//
