LOCUS Exported 15840 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Anopheles gambiae ORCO enhancer/promter to drive QF2 expression in Anopheles transgenic mosquitoes. ACCESSION . VERSION . KEYWORDS pBac-DsRed-ORCO_9kbProm-QF2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 15840) AUTHORS Riabinina O, Task D, Marr E, Lin CC, Alford R, O'Brochta DA, Potter CJ TITLE Organization of olfactory centres in the malaria mosquito Anopheles gambiae. JOURNAL Nat Commun. 2016 Oct 3;7:13010. doi: 10.1038/ncomms13010. PUBMED 27694947 REFERENCE 2 (bases 1 to 15840) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..15840 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin complement(3..458) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(140..161) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 352..371 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 585..607 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 599..616 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 600..616 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" repeat_region 746..808 /label=piggyBac right (3') inverted repeat /note="piggyBac transposon-specific inverted terminal repeat sequence (ITR)" regulatory 1288..1297 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1294..1971 /codon_start=1 /product="improved tetrameric variant of DsRed fluorescent protein" /label=DsRed2 /note="mammalian codon-optimized" /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH LFL" primer_bind complement(1474..1494) /label=DsRed1-N /note="DsRed1, reverse primer" primer_bind 1886..1909 /label=DsRed1-C /note="DsRed1, forward primer" polyA_signal 2092..2173 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2129..2148) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2183..2202 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" repeat_region 13166..13200 /label=piggyBac left (5') inverted repeat /note="piggyBac transposon-specific inverted terminal repeat sequence (ITR)" primer_bind complement(13691..13707) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(13691..13707) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(13704..13726) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 13715..13731 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(13739..13769) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 13784..13805 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(13922..13939) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(14093..14681) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(14173..14192) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(14852..15712) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 15475..15494 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(15713..15817) /gene="bla" /label=AmpR promoter ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 601 taaaacgacg gccagtgagc gcgcctcgtt cattcacgtt tttgaacccg tggaggacgg 661 gcagactcgc ggtgcaaatg tgttttacag cgtgatggag cagatgaaga tgctcgacac 721 gctgcagaac acgcagctag attaacccta gaaagataat catattgtga cgtacgttaa 781 agataatcat gcgtaaaatt gacgcatgtg ttttatcggt ctgtatatcg aggtttattt 841 attaatttga atagatatta agttttatta tatttacact tacatactaa taataaattc 901 aacaaacaat ttatttatgt ttatttattt attaaaaaaa aacaaaaact caaaatttct 961 tctataaagt aacaaaactt ttatcgaatt cctgcagccc gggggatcca ctagttctag 1021 tgttcccaca atggttaatt cgagctcgcc cggggatcta attcaattag agactaattc 1081 aattagagct aattcaatta ggatccaagc ttatcgattt cgaaccctcg accgccggag 1141 tataaataga ggcgcttcgt ctacggagcg acaattcaat tcaaacaagc aaagtgaaca 1201 cgtcgctaag cgaaagctaa gcaaataaac aagcgcagct gaacaagcta aacaatcggg 1261 gtaccgcggg cccgggatcc accggtcgcc accatggcct cctccgagaa cgtcatcacc 1321 gagttcatgc gcttcaaggt gcgcatggag ggcaccgtga acggccacga gttcgagatc 1381 gagggcgagg gcgagggccg cccctacgag ggccacaaca ccgtgaagct gaaggtgacc 1441 aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcca gtacggctcc 1501 aaggtgtacg tgaagcaccc cgccgacatc cccgactaca agaagctgtc cttccccgag 1561 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggcgac cgtgacccag 1621 gactcctccc tgcaggacgg ctgcttcatc tacaaggtga agttcatcgg cgtgaacttc 1681 ccctccgacg gccccgtgat gcagaagaag accatgggct gggaggcctc caccgagcgc 1741 ctgtaccccc gcgacggcgt gctgaagggc gagacccaca aggccctgaa gctgaaggac 1801 ggcggccact acctggtgga gttcaagtcc atctacatgg ccaagaagcc cgtgcagctg 1861 cccggctact actacgtgga cgccaagctg gacatcacct cccacaacga ggactacacc 1921 atcgtggagc agtacgagcg caccgagggc cgccaccacc tgttcctgta gcggccgcga 1981 ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc 2041 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt 2101 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 2161 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc tcgagtgatg 2221 caaattgttc ggaagaattg gaaacaacac cccaacactg ccccaactgt caccgactca 2281 ttgtgccttt gcatccatgc ggagcggtaa agataactgc ggcaaggaca cttcccaccc 2341 tagtgtctag aattaagtta attaacatgc cgcgctacct cttaatctaa acatgttccg 2401 gaaacgagtt ctttcatacg gaagggcttt gttttttttt tttcgcccaa gtcgtgtgtc 2461 aacaaagtgg aaaggtctag gggctgtttt tatgttttag gcttttcacg tcgcgcaaaa 2521 cgataaataa acgcggtcgg cggattgacg gcagcaaaca gatagataaa atggcttcat 2581 aaagcaaaaa cttctcctcc tgctcttcac ggctcttcaa atcccctgag ttaatagcag 2641 catccctggt ttgtgtattt tgtttttctt cgcccatttt ggtccaccaa aaagctaggc 2701 ggtcgatgaa gtactagcag catcgaaagc aaataaattc tcgacgcatg gtgccccatt 2761 tttgggacgt gttattacgc acgtaaagct gagtgcatgg ggaagctgca cagaggaaag 2821 ataaaagttg tttggattgg agtttggatg atcaatacgg cgtagccgta gaatgttcat 2881 agttttgtca aagtaagcga aagtttcagc gcggaagcgc tcgttaaagg acaggagcaa 2941 gttgtacaca aagaaattac ttttgcgtca cgtaaatcac actaacgttg gggtgtggga 3001 cggtggattg cggattgcat acttgtcggg ggcgtttgga agaaggaaac gcctgaaagt 3061 aggctacttt tgggacaacg atgtcatttt agttatccgt tgatctttct tcatgccgtt 3121 ttgtcgtagt gcttcagaaa aatacaaata ttctttaaaa aaaacggcca aaagaagaaa 3181 aattgcataa cacaataatg tgacttttca aattactatt tagtacatcg cattcactgt 3241 ctttaaaatg ttctttatgg gttattttcc agtcactatc tcaagctaca aaaagcgtcc 3301 ttcatttatc tagcatggta gttgtctatc tattcagttt ttaagaaccc ttggaatcga 3361 tcgcacagcc tttcacgacg atggcattga gcttaaccat cagctttgtt gactttgcct 3421 catcctttgt atgggtatgt cgtacattaa acgattaaga ttattgtttg gtcaacatca 3481 gttgatagtc aatcgatcaa ctcgatatgc actgtaaact ggccgccatc cgagaagccc 3541 tggaagcgtg cagcaaagca ccccgaagat gaccgtacga gtgtgtgtgg ggcaaacagt 3601 tcccatgcgc ggctacggga agggaaaacc gtggagaaac cgagtgggtt ttgtctggta 3661 atcagtacgg tacacagcgc ctgattgcaa aatccatcca aacgatgatt agatgaaagg 3721 atttccggac cttcggccgg ccaaactgtc cggtcccggc tacatctgca cggcgtcatt 3781 cagtcccgta ccgatggccg agcacgccgg tacggacggt gtgctaactt ttctgtccat 3841 cgacgaacta tccactcagc ccaaaacagc ccttcgtggt gtgccatgtc atcgagcaac 3901 ggttggaatc ggatggaaac gccatcgaac gtaagtagat cagaaagccg aaaccctgcg 3961 caacttatca tactggctgc tagctgttgt gcatgtgtgt gtgatgttaa tggggataaa 4021 atcctacaaa ggtgaatcca tctttcctgc aggcaccatg caaagtaatg cagcgtcgat 4081 agctcggtgc accatccatg gtactctacc tcgctttgat ctattagatg cctctgcttg 4141 tagtgtgtct aatacgcgca cgatctatct gtttaaaatg caatattcta ccatcacctc 4201 caccaccagc cacccacagc actgcttcat gtttctgctg cctgcacagt ttatcgtacg 4261 ttcgttcgtt tgcatttgat aaacgatacg tgctatttca tatccattac ggtaatgaga 4321 taaagtcgtt accggcaccg ggcgttcgat agaaataccc gttcgaacag caatctccgt 4381 caagctactg gtgcagccgc gattcggaat gcagctgcgg aatgcataat agagaaattt 4441 atggctcaat ttacaacggc cgcactgatt atgctgacat aatctaataa atgcctttcg 4501 ggggtaaact gtatttctaa tcgttgaatt gaaggctgtt tgctgcagtg gctgtgagct 4561 aatttggtaa aaaatagagc ttcgagagac ttgttttcaa atagcaatca aaccacattt 4621 tctgcgaaca catttatatt ccacaccaag cacaaatcat ccagtgtcta acaccactgc 4681 acaagcatca gctcccttgt gttcgcacag tgcgcaccaa cgcgtcaagc tgtcttttta 4741 tagaatgcta ttagatcgta gcgcagtcct tccacccctt tccgtagccc gtacgcaacg 4801 cgacactttg ctgacactgt gcccggcaat atgtgcccga caaacaacgc gtctgttgac 4861 agctattttg gggcttcaca tacaacacca gtctacgaga cactggaaac gaatccgcct 4921 ttttgctcta gaacaaagtc catcagcatc aagtggtatt cagcccattt cattgattat 4981 taaaatcact atctgcacgg agacagcgcg tggtattaaa atgcgaaacc acacgcacac 5041 acaacgtagc agcgtttgcc ttattgcgtt cgcatcgaag catacggacc gaccgcacag 5101 gcaacgaatg ctcgatcctt taacttgaac cgctgagtga aaagataatg aaccatgaaa 5161 tattaaccat ttattacaat cgttccattt ccagctgtaa atcaatcaat acataaaccg 5221 tgcttcgctt taatgggcag caccctgtcc cctccgttaa agccgcttgg ttgagcaaaa 5281 atcactgcgt gcgtgtgcgt gtgtgtataa tatcctccgc aaagcttaat ccaccccagc 5341 catgtgtgca ctggcgctgt ttgcacagca tagacgcctt tcgaaatttg ttttgaatat 5401 aaatatcgta aagcaatcca tgcctttatg aagcagagta cgacctctcg tcggtgccat 5461 tcaaaactct gctctgcatt ttatcttttg gttgtgccgc aaagggaagg gcgagcaaaa 5521 aagaaaagcc caaacaacaa gaacacgtta gcgatgaagt aaatccatca cccatcggaa 5581 gcatcgaagg gcaggaaaag aacgccaact gagctagcct gctagagcca gccgagtgcc 5641 gatacgcagg ccaggctcgt gattgcataa cacagcctcg tgatgcttcc tttgtgtggg 5701 agcgtgttgt gccgtggggg aagcggccga tggatgcggg aagattgtcc ctttccctag 5761 cttgtctcgc gtgtgtgtcc tgccccggtg agtgactcac tgctcgtcgt ggcgttcgtc 5821 gtctatcgtc gtctgagcga gtgtatgggc gagtcggcgt ctctcaccct ttggccgtgg 5881 ccgaacgcag acaaatttgt aaccaaccac ccttccattc tgccactcct ttctgccact 5941 tccccttgtc ttccgcactg ggagcaagtt gttgttggtg gaagaaattt cttcgaacgt 6001 ttctcccttg gagcttgggt ttccgatgtc ttccgacact tatccatcca tacgcatacg 6061 ccaacccgct cagccttatt tggcgtgcat tcgggtagtg caaaccggcc aggcatcaaa 6121 tatgatatcg caaaaaccca ttggcattcc accacgtgct cagccgcccg gacacgattg 6181 gcttgcgaca taagccgatt accggagatg gttttggtgc tcatatttcg tacaaggtgt 6241 aattcccgcc gacatcacca taggccgtcg tttgtttgat taattagatt gttatttctg 6301 tgctttccac tcttccccat ttttcaccgc ttttcctgga actcgataat atttccacca 6361 tgacgagccc ggcaagggca caaaagtagg cacagcaaaa aaaaacaata caaaaggaaa 6421 gggatatgtc actaacgcta tgtttattta ctttgtgtct ttacactcgg ctgggtctgt 6481 attccaaagg gttttgcagg gttttcgcga aggatttccc tcaaggtaga gcagcttcga 6541 cacgaatttc acttaaattt ttttatcgtt ttcaacactc gcaattagct ttcacaaggc 6601 tggtggttaa cacgtttttt agtattattt ctttaaaata aaaagcacac actccaccac 6661 catttaccgt ttacttgttg ataacgcctt cccaaacaaa gcacctttcg ggactgtcga 6721 gacaaaaaca cgtgagctta tcagagtcgc aggggtggta tgggggaaaa aacggaaccg 6781 aacgaaacac taaacggttc gagcgaccat gcgcctccac cagtccacca aaaacgcttc 6841 ttttatttac acaccacgct ctgcgttcag tttcaaagcc aacgccaatc actacgcatt 6901 caacactctt tattgctcag accactatca tgggaacggg gaacacaaat agaacgcgtc 6961 atcaaagggg cacaacaaaa aagggggata aaacaaccat ttccaaaagt ggaaggaaaa 7021 aaagatgcta aacgcacagt aataaaaaaa agaacactct gtcacaccgg gacgcttcac 7081 caaaaattcg tggataggtg atggcaatgt gtgcgtgtgt gttcgctccc tttacgtatg 7141 tgtgtgtgtg cttgtgtgta tgcgcgtaat agaaagatgc cgttttcgga aaaccgtacc 7201 acaaccgtac agacacacac gcacgcacgc acacacaccg tcggtctagt gcgtccgcgt 7261 gaaagcaagg ttcatctgcg actagctcat ctcgtgtgcc tcgatgacgg tggcactggc 7321 cccacagctt ttcacgatgg taggagtagg agcgcaagga tgaagaacgc acacagaacg 7381 tcgtcgtgtt tcgtcgaccc ccggagccga gagcagctcc cccggaaacg gaactttatc 7441 tcacccttat cagaacggcc aacgtgtgcc gcgttcgtga gaaagagagt gagagcaacc 7501 gacaagcaac gaacgtgagt gcgctggaga gtgagcagct cccgaacgga actcacgcgt 7561 tgaagggaga gcgtgtttcg ttttctttca ttttcttttg tattttttta caaaacattg 7621 taaatattcg tacagcattt tgctacaaca acatcaacgg cagcagcaac aaaaaaaagt 7681 tgggtgaaat ttgctcggac agttttccgt gaaaacagct gagtacggca cggcacgatt 7741 gatagacaca gccagtccgg agaccattcg cggagggttg cgttcatgtg cgacctctgc 7801 gattttcgta cgcaaagccc gccagccgtg tgtgaacgag ttaattgatt tttaacgctg 7861 tgtatcggtt ttgtaatcct gtcccttgca cacgtgtctt tttgtgtgta cggcgttgag 7921 caagccattt taaggcgctt tttatgagcc ttaaagagcg cttctgtgtg attttttatt 7981 tttctatttc gaccattata cttcgccgtc acacatgcta tgagtgtgtg gtttttgttt 8041 tccccctcaa agattatagc cttcagagag caatgaattt gaaatcaacc atccggcgcc 8101 gcttttcgct tcctttgcca ctcacaccga gcagcccccg tgctagagtg ctgagtgatc 8161 gctcagcatg tttagcgaca gtcctttttt cgcaaacaac ttccacttaa caaaccctac 8221 caaagcatgt tttaaacatc gttattatga ttttatctcc caaccggtga catgctgggc 8281 gcaaaacacc accttccgct tgattaatgg gtgcgacgga gagcagtgtg atggactgca 8341 atgagggaag gggggccacg aaatcaatta agggtttaat taaaatggcg ttcccgctaa 8401 cgccgacgcg tcacggcttc acgccaaacg attcatcttt cgagacgatt attactctgc 8461 gcgcttcagc atatttctgt ccctgcagtc ttatgatcga cattcctggc gcagcgagcc 8521 agagagagag taaaatgatg tgccattaac cgcccgggac gggttgacgg attggcgaac 8581 agaacacaca cacagagttc cgggtttcct cgaagtaggg tttggtctgg agcgctcacc 8641 gcccaagaga ctcgagacga agagcgatga aagaataata tgtgccgaac gtagactcca 8701 cgcgggggtt aacatgaaac gatgatcgct tcccgctaga catagacaca gattcttcgc 8761 ctccctcagc acgtcagcac atttgcacag gatatggacc cccctccccc ctcctctaca 8821 gaacattcca accttcttgg gtgtgatagt tacggttagc ttcaagtgtc tcgtaaaact 8881 aacgtgctgt cgcccgcatc tatctctatc tcaagccgga atcggccgcc atgaaagcgg 8941 ctgtggaaag tgaaagttac acagcacacc aaaggggccc ttcatcatgc ttttctcgtt 9001 ctccatcatc ctcgtagaac aatgtcccgg atgagtttct gctctttctt ttttactctc 9061 atgtcagccc atggtgatgg taaatgcagg gaaaaaaacg agggaagccg agagttccgt 9121 tttgccatca ccatacccaa ctggaggtgt ataaaatatg cctatttaag cactggcaga 9181 cacccacacc gaccgcccga aaggcgacca agtgtgaagg tcctaaattg tgtcctccgc 9241 tctgtccgct ttgccgcttc cttcaaaacg gcccttctgg agcacgtcga cgacacgaca 9301 tgacactctg ggactgggga tagcgataag cgttctgtta aacacagcac attttgtgtg 9361 atgatcacgg tggaggagcc tgctgcctat gaccaacccg tgccttcaat cttcaccgac 9421 aagcgagctg gtaggctcgt gttttcatca caagtcgtgc aggagcgtgt gccgctcaag 9481 gtaaaggtcg ttccttttgg ggccgagttt taattttaaa ccctcatttg cgtcacaacc 9541 ccgttacctg gccccttttt cgaattggaa aaaggcaagt ggttaagcaa gacccaatga 9601 ggcaggaagg attgcactgt gtgaggacaa tcaaacggaa agcccaaaac acacattagt 9661 tttactttcc cttttttgct ttgataagac aagcaataag acaaacacac ttactctcac 9721 atacacactc gtatatatat ataatttcac acacacacac tagaacgagt ccttccgtcg 9781 tagttgacag cgctaaatat ttaatcgtgg gatcagcgtc cggtgacaaa cgagggacaa 9841 atacgccttg gagggagatc gctcaagacg acaagctacg cggcttcgct tcagtttctt 9901 tcgtttttgt agcatatttt tttaaaataa ttattattca caaaatacaa gaccccgata 9961 agcaattaat atttaccagt ctaaccttct tcgctcaggt acaatgtcaa tcaccaatac 10021 acacaaacgt gtgccgtgct tataaagacc tccattttac gctcccgatc gatgctgcac 10081 gtcgtaaacg gaaagtgtgg catcgagtgg ttgttagcat aaaagattaa cgcacttaaa 10141 actcttccac cctcattctc gagtctataa accagcgtgg ggagcatcca aaccacacac 10201 tcacacacat acacacacgc acgcatccac atattagtcg taagtgatgg cggtgggaaa 10261 atgcggccat tagctgccca gacggaacag atgttgcgct gggaaagttg gactcctcaa 10321 gccaccagca agatcgggac gggtacacat gtaccgagaa cgcacactcg cgccattggt 10381 tgatgtcacg ggacgctcgg taagcgtatt cctagcggcc acgtaagcca cccccaagga 10441 caaacccaac ttctgtaccg gatgatcaga atagcgtcga cacttcttac attcgattga 10501 tacgtcgagc ggaaaggacg tatcaagtcg attcgtctat cagtgtcgga acgatagtga 10561 tagaaatcta caggcgccaa tcataaaccg tttactgctc gcgataggac acgctttgct 10621 aacgtcttgt gcatcgcgaa aactagtgat tggagtgtgg ttttgtagct gtttgtcgtt 10681 ctgtgctcca cgttgactct gtgtgtgtgt gcgctgaatt caacaagact tttctgcaac 10741 agcatcattt gcaaagaata accggcgcga cttacgcggt ctgacttgct ggtgcgctgc 10801 tttgtacggc aaacggctac acaagcgaat cgaattattt tcctatcacg ctgcgcttac 10861 cagcgcctgc tggtaggcaa agaatgtgca aagtttcatt tggcttggtt cgtctgcttt 10921 ttgctgtgaa cgtgtgcacg gttgcatcgc taaggtttcg gtgtgagccg agaagttgca 10981 gatcgaaatc tctttctgtg tgtgtgtgcc gtgggaagca ttgtgtttag tgagaagtga 11041 aaagaaaagt gctgaaaagt aagtattcga tgtagcttca acctttacac ttgacaaggc 11101 ggagatctca accatttttc tagttcgaac gttaaacgga acatttgatc ctttcaaaac 11161 tgtgtgactg tgtttgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgcaat cgccccccat 11221 gtattccaaa tatttgcttc tcgcaaatca ggtcacaaag ctgtgctcaa tgttggcaca 11281 atcaaagctt tccccattcg tctgcccaag cctagctcaa ttgtttgtac gagagatttt 11341 accgtttggc tccaagcatt tgcaattgtc ctttcctgta cgcttgccgc cgcttcactg 11401 cgcccttcta agtacacaga gcacacacag cttaactctt ccctgtagct gtacggcttg 11461 gtgtctgtta cctaaaaatc gataaatcaa ttagtctctt gccgaccaca ccaaacgacc 11521 actacaaacg agcccgactc gacccgttca cttcccgttc gcaggccggc caacatgcca 11581 cccaagcgca aaacgcttaa cgctgcggct gaggctaacg ctcatgccga cggacacgcc 11641 gacggaaacg ccgacggaca cgtggccaat acggccgcgt cctcgaataa tgcgaggttc 11701 gctgatctca ctaacatcga tactccgggt ctgggaccca caactacgac cctgctcgtg 11761 gaaccagcac gctcaaagcg tcaacgagtg tcccgcgcat gcgaccagtg ccgtgcagcc 11821 cgagagaaat gcgacggaat acagcctgcg tgtttcccgt gcgtttccca gggaaggtcc 11881 tgcacttatc aggcttcgcc gaaaaagagg ggagttcaaa ccggttatat tcgtacgctg 11941 gagctcgccc tcgcctggat gtttgaaaat gtcgcgcgtt ccgaagatgc cttgcataac 12001 ctcctcgtcc gtgacgccgg acaaggatca gctctgctcg ttggtaaaga ttcgccggct 12061 gccgagcgac tccatgcccg ttgggctact agccgtgtca ataagagcat tacccgcctc 12121 ctccgtcagt tggagctccc tcctaccgcc acggctacgg cctcgataat gccgcacgtg 12181 atggagcagc ctctcagtac cagcattaac cccgtcaacg accgcttcaa cggtattccc 12241 aaccccactc cgtataactc cgatgcagct ctcgatgcta tcactcagac caacgattat 12301 ggaagcgtaa atacacatgg tatcctctct acttacccgc caccggctac gcaccttaat 12361 gaagcttccg tcgctctcgc tcccggtggc gccccccccc gaccgcctcc tccgtatgtt 12421 gacagcacga ccaatcaccc gccgtaccac tcgaatctgg ttccaatggc gaactttggt 12481 tactcgaccg ttgattacga tgccatggtt gacgatttgg ctagcattga atacacggac 12541 gctgtggatg tcgacccaca gtttatgacc aatctgggat tcgttcctgg atgtaacttc 12601 tccgacatta atacatacga acagtgatga cgtctatcga taccgtcgac taaagccaaa 12661 tagaaaatta ttcagttcct ggcttaagtt tttaaaagtg atattattta tttggttgta 12721 accaaccaaa agaatgtaaa taactaatac ataattatgt tagttttaag ttagcaacaa 12781 attgatttta gctatattag ctacttggtt aataaataga atatatttat ttaaagataa 12841 ttgcgttttt attgtcaggg agtgagtttg cttaaaaact cgtttagatc cgatatctat 12901 aacaagaaaa tatatatata ataagttatc acgtaagtag aacatgaaat aacaatataa 12961 ttatcgtatg agttaaatct taaaagtcac gtaaaagata atcatgcgtc attttgactc 13021 acgcggtcgt tatagttcaa aatcagtgac acttaccgca ttgacaagca cgcctcacgg 13081 gagctccaag cggcgactga gatgtcctaa atgcacagcg acggattcgc gctatttaga 13141 aagagagagc aatatttcaa gaatgcatgc gtcaatttta cgcagactat ctttctaggg 13201 ttaatctagc tgcatcagga tcatatcgtc gggtcttttt tccggctcag tcatcgccca 13261 agctggcgct atctgggcat cggggaggaa gaagcccgtg ccttttcccg cgaggttgaa 13321 gcggcatgga aagagtttgc cgaggatgac tgctgctgca ttgacgttga gcgaaaacgc 13381 acgtttacca tgatgattcg ggaaggtgtg gccatgcacg cctttaacgg tgaactgttc 13441 gttcaggcca cctgggatac cagttcgtcg cggcttttcc ggacacagtt ccggatggtc 13501 agcccgaagc gcatcagcaa cccgaacaat accggcgaca gccggaactg ccgtgccggt 13561 gtgcagatta atgacagcgg tgcggcgctg ggatattacg tcagcgagga cgggtatcct 13621 ggctggatgc cgcagaaatg gacatggata ccccgtgagt tacccggcgg gcgcgcttgg 13681 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 13741 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 13801 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 13861 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 13921 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 13981 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 14041 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 14101 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 14161 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 14221 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 14281 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 14341 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 14401 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 14461 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 14521 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 14581 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 14641 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 14701 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 14761 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 14821 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 14881 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 14941 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 15001 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 15061 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 15121 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 15181 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 15241 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 15301 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 15361 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 15421 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 15481 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 15541 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 15601 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 15661 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 15721 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 15781 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac //