LOCUS       Exported               11897 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    pUG-124
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11897)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 11897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11897
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     15..34
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15..33
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    34..58
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             112..129
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3677..3763
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDY"
     CDS             3740..3766
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3779..3865
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDY"
     CDS             3842..3868
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4348..5001
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     4959..4981
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             5008..5025
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(6846..6864)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6860..6907
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      6944..7399
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7031..7050)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7241..7262
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7491..8306)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8214..8233
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8298..8317
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      8428..9016
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8917..8936
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9170..9187
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9202..9341
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9267..9286)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9427..9449
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(9443..9634)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(10443..11525)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     11487..11506
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(11526..11603)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     11809..11828
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cgatcccgcg aaattaatac gactcactat aggggaattg tgagcggata acaattcccc
       61 tctagaaata attttgcctt aactttaaga aggagatata ccatgggcag ccaccaccac
      121 caccaccaca gcggaatgca gatctttgtt aagactctca ccggaaagac aatcacccta
      181 gaggtggaaa gctccgacac catcgacaac gttaaggcca agatccagga taaggagggc
      241 attcctccgg atcagcagag gcttattttc gccggcaagc agctagagga tggccgtacg
      301 ttggctgatt acaatatcca gaaggaatcc accctccact tggtcctcag gctccgtggt
      361 ggttaagctt gcaattaact ttaagaagga gatatacaat gcttcacaag cgagctagtg
      421 aagctaacga taagaacgat aataccatca tcggttccga tttagcatca tctaagaaac
      481 gccgtatcga tttcactgaa tcttcgtctg ataagtcatc ttcgatttta gcttctggta
      541 gtagcagggg ttttcacggc gatagcgtcg ttcagcaaat cgacatggct tttggtaatt
      601 cgaaccgtca ggagattgat gaagatctgc acagtcgcca gctcgccgtc tatggtcgtg
      661 agactatgag gcgtctcttt gcttccaatg ttctcatctc ggggatgcac ggtcttggtg
      721 ccgagattgc caagaatctt atacttgctg gtgtgaagtc tgtgaccttg catgatgaaa
      781 gagttgtaga gctatgggac ttatcaagca actttgtttt ctcggaggat gatgttggca
      841 agaatagagc agatgcttct gttcagaagt tacaggatct taacaatgct gtggttgtct
      901 ctagcttgac caaaagctta aacaaagagg atctttctgg cttccaggtt gttgtattct
      961 ctgacataag catggaaaga gcaatcgaat ttgatgacta ttgtcacagc catcagcctc
     1021 ctatcgcttt tgtcaaggct gatgtcaggg gtcttttcgg ctctgtcttt tgtgattttg
     1081 ggcctgaatt tgcggttctt gatgttgatg gagaggaacc ccatacaggc atcattgcgt
     1141 ctatctctaa tgagaaccag gcatttattt cctgtgtcga cgatgagagg cttgagtttg
     1201 aagatggtga cctagttgtt ttctcagaag ttgagggtat gacagaattg aatgatggga
     1261 aaccaaggaa gataaaaagt acacggccat attcattcac ccttgatgag gacactacta
     1321 actatggaac atatgtgaag ggtggtattg tcactcaagt gaaacagcca aagttactga
     1381 atttcaagcc attgagggaa gcgcttaagg atccagggga ttttctattt agtgatttct
     1441 ctaagtttga tcggcctcca ctccttcact tagcattcca ggcacttgat cattttaaag
     1501 ctgaagctgg cagatttcct gttgctggct cagaagagga cgctcagaag cttatatcga
     1561 ttgccacagc catcaatacc gggcagggtg atttaaaggt ggagaatgtt gatcagaagc
     1621 ttctaagaca cttttccttt ggagccaagg ctgtcctgaa tcctatggct gcaatgtttg
     1681 gcggtattgt tggacaggag gttgttaaag cttgctctgg aaaatttcat cctctcttcc
     1741 agtttttcta ctttgattca gtggagtcac tcccctctga acctgtggac tcgagtgact
     1801 ttgcaccaag aaacagccgg tatgatgccc aaatatctgt atttggggcc aagttccaga
     1861 agaaacttga agatgctaaa gttttcacag tagggtctgg tgctcttggc tgtgagttct
     1921 tgaaaaattt ggctctgatg ggggtttctt gtggaagcca agggaagctg acagtgacag
     1981 atgatgatat cattgagaag agtaacctca gtcgtcaatt tctgttccgt gactggaaca
     2041 ttggacaggc taaatccaca gttgctgctt cggctgctgc tgttataaat cctcgcttca
     2101 acattgaggc cctgcaaaac cgtgttggcg cggagactga gaatgtattt gacgatgcct
     2161 tctgggagaa cttaactgtt gtcgtcaatg cactggataa tgttaatgcg aggctctatg
     2221 ttgattcgag gtgcttgtat ttccagaagc ctctccttga gtctggaact cttggtacaa
     2281 agtgcaacac acagtcggtt atcccacatc taactgaaaa ttacggtgcc tcaagggacc
     2341 caccagagaa acaggccccc atgtgtacag tgcattcctt cccgcataac attgatcact
     2401 gtttaacttg ggctcgctct gagtttgagg gtctgctgga gaaaactccc gctgaagtga
     2461 atgcatatct ttctagccca gttgagtaca ctaactcaat gatgagtgct ggtgatgctc
     2521 aggcgaggga cacattggag aggattgttg agtgccttga aaaggagaag tgtgaaacct
     2581 tccaagactg cttaacctgg gcacgactga ggtttgagga ttactttgtg aaccgtgtca
     2641 agcaattgat atacacattt cctgaagatg ctgcgactag taccggagct ccattctggt
     2701 ctgctccaaa aagattccct cgtccactcc agtactcgtc gtccgaccca agtctcctta
     2761 actttatcac agcgactgct attttacgag cagagacatt tgggatcccg atacctgagt
     2821 ggactaagaa cccaaaggaa gcagcagaag ctgttgacag agtgatagtc ccagactttg
     2881 agccaaggca agatgcaaag attgtgactg atgagaaagc taccacttta accactgctt
     2941 cggtggacga cgctgcagtc atcgacgatc tcattgctaa gatcgaccag tgtaggcata
     3001 acttgtcgcc agacttcagg atgaaaccta ttcagttcga aaaggatgat gatacaaact
     3061 atcacatgga tgtgatagca ggactagcca acatgagggc gagaaattac agcatccctg
     3121 aagtggacaa gctgaaagca aagttcatag cagggagaat catcccagcc attgcgactt
     3181 caacagccat ggctactggg ctggtctgcc tcgagctgta caaggtcctt gatggaggac
     3241 ataaagtgga agcttacaga aacacatttg ccaacctggc acttccacta ttctccatgg
     3301 ctgaaccact tccaccaaag gtggtgaagc accgtgacat ggcttggaca gtttgggaca
     3361 gatgggttct caagggaaac ccaactcttc gtgaggtgtt gcagtggctg gaggacaaag
     3421 ggcttagcgc atacagcatc tcttgtggaa gctgtctact gttcaacagt atgttcacaa
     3481 ggcacaagga gaggatggac aagaaggttg tggatctcgc tcgggatgtt gcgaaagtcg
     3541 agttaccgcc ttatcgcaac catcttgatg tagtggtggc ttgtgaggat gaagatgaca
     3601 atgacgtcga tattcctctc gtctctatct acttcaggtg agcttactat taactttaag
     3661 aaggagatat accatgtacc catacgatgt tcctgactat gcgggctatc cctatgacgt
     3721 cccggactat gcaggattgt atccatatga cgttccagat tacgccacta gagctgctta
     3781 cccatacgat gttcctgact atgcgggcta tccctatgac gtcccggact atgcaggatt
     3841 gtatccatat gacgttccag attacgcaat ggcttcgaaa cggatcttga aggagctgaa
     3901 ggatctccag aaagaccctc caacctcctg cagtgcaggt ccagttgctg aggacatgtt
     3961 tcattggcaa gctacaatta tgggtcctgc agagagtccg tattcaggcg gtgtgtttct
     4021 cgttaccatt cacttccctc cggactatcc attcaaacca ccaaaggttg catttaggac
     4081 gaaggtgttt caccctaata tcaacagcaa cggaagcatt tgccttgaca ttttgaaaga
     4141 acaatggagc cctgccctca ccatttccaa ggtgttgctc tcgatatgtt cgctgttaac
     4201 agatccaaat ccagatgacc ctttggtacc agagattgca cacatgtaca aaaccgacag
     4261 agccaaatac gaggctactg caagaaactg gactcagaag tatgccatgg gctaagcttt
     4321 tacttaactt taagaaggag atataccatg tcccctatac taggttattg gaaaattaag
     4381 ggccttgtgc aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat
     4441 ttgtatgagc gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag
     4501 tttcccaatc ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc
     4561 atacgttata tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag
     4621 atttcaatgc ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat
     4681 agtaaagact ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa
     4741 atgttcgaag atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct
     4801 gacttcatgt tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat
     4861 gcgttcccaa aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag
     4921 tacttgaaat ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt
     4981 ggtggcgacc atcctccaaa atcggatctg gttccgcgtg gatcaatggc gggtatcaat
     5041 agagcgggac catcgggcgc ttattttgtt ggccacacag accccgagcc agtatcgggg
     5101 caagcacacg gatccggcag cggcgccagc tcctcgaaca gtccgcaggt tcagccgcga
     5161 ccctcgaata ctcccccgtc gaacgcgccc gcaccgccgc caaccggacg tgagaggctt
     5221 tcacgatcca cggcgctgtc gcgccaaacc agggagtggc tggagcaggg tatgcctaca
     5281 gcggaggatg ccagcgtgcg tcgtaggcca caggtgactg ccgatgccgc aacgccgcgt
     5341 gcagaggcaa gacgcacgcc ggaggcaact gccgatgcca gcgcaccgcg tagaggggcg
     5401 gttgcacacg ccaacagtat cgttcagcaa ttggtcagtg agggcgctga tatttcgcat
     5461 actcgtaaca tgctccgcaa tgcaatgaat ggcgacgcag tcgctttttc tcgagtagaa
     5521 cagaacatat ttcgccagca tttcccgaac atgcccatgc atggaatcag ccgagattcg
     5581 gaactcgcta tcgagctccg tggagcgctt cgtcgagcgg ttcaccaaca ggcggcgtca
     5641 gcgccagtga ggtcgcccac gccaacaccg gccagccctg cggcatcatc atcgggcagc
     5701 agtcagcgtt ctttatttgg acggtttgcc cgtttgatgg cgccaaacca gggacggtcg
     5761 tcgaacactg ccgcctctca gacgccggtc gacaggagcc cgccacgcgt caaccaaaga
     5821 cccatacgcg tcgacagggc tgcgatgcgt aatcgtggca atgacgaggc ggacgccgcg
     5881 ctgcgggggt tagtacaaca gggggtcaat ttagagcacc tgcgcacggc ccttgaaaga
     5941 catgtaatgc agcgcctccc tatccccctc gatataggca gcgcgttgca gaatgtggga
     6001 attaacccaa gtatcgactt gggggaaagc cttgtgcaac atcccctgct gaatttgaat
     6061 gtagcgttga atcgcatgct ggggctgcgt cccagcgctg aaagagcgcc tcgtccagcc
     6121 gtccccgtgg ctcccgcgac cgcctccagg cgaccggatg gtacgcgtgc aacacgattg
     6181 cgggtgatgc cggagcggga ggattacgaa aataatgtgg cctatggagt gcgcttgctt
     6241 aacctgaacc cgggggtggg ggtaaggcag gctgttgcgg cctttgtaac cgaccgggct
     6301 gagcggccag cagtggtggc taatatccgg gcagccctgg accctatcgc gtcacaattc
     6361 agtcagctgc gcacaatttc gaaggccgat gctgaatctg aagagctggg ttttaaggat
     6421 gcggcagatc atcacacgga tgacgtgacg cactgtcttt ttggcggaga attgtcgctg
     6481 agtaatccgg atcagcaggt gatcggtttg gcgggtaatc cgacggacac gtcgcagcct
     6541 tacagccaag agggaaataa ggacctggcg ttcatggata tgaaaaaact tgcccaattc
     6601 ctcgcaggca agcctgagca tccgatgacc agagaaacgc ttaacgccga aaatatcgcc
     6661 aagtatgctt ttagaatagt cccctgagct tcagagcttt taagccagcc ccgacacccg
     6721 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     6781 gctgtgaccg ggatgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc
     6841 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
     6901 ttttttgctg aaaggaggaa ctatatccgg attggcgaat gggacgcgcc ctgtagcggc
     6961 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     7021 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     7081 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     7141 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     7201 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     7261 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     7321 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     7381 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     7441 gtttattttt ctaaatacat tcaaatatgt atccgctcat gaattaattc ttagaaaaac
     7501 tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt
     7561 tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca
     7621 agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc
     7681 ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt
     7741 gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc
     7801 tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg
     7861 agtcgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg
     7921 cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat
     7981 acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta
     8041 cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc
     8101 atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc
     8161 gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga
     8221 gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg cctagagcaa
     8281 gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac
     8341 agttttattg ttcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     8401 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     8461 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     8521 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     8581 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     8641 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     8701 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     8761 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     8821 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     8881 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     8941 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     9001 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     9061 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     9121 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     9181 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     9241 ttcacaccgc aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     9301 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
     9361 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     9421 ccgactccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
     9481 agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat
     9541 ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg
     9601 ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc
     9661 tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg
     9721 atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc
     9781 ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag
     9841 gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg
     9901 gcgctgactt ccgcgtttcc agactttacg aaacacggaa acccaagacc attcatgttg
     9961 ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg
    10021 attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga
    10081 gcacgatcat gcgcacccgt ggggccgcca tgccggcgat aatggcctgc ttctcgccga
    10141 aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata
    10201 ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga
    10261 cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaacaca gtcataagtg
    10321 cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca
    10381 agggcatcgg tcgagatccc ggtgcctaat gagtgagcta acttacatta attgcgttgc
    10441 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
    10501 aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt
    10561 gacacgggca acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg
    10621 tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata
    10681 taacatgagc tgtcctcggt atcgtcgtat cccactaccg agatatccgc accaacgcgc
    10741 agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc
    10801 atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg
    10861 gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta
    10921 tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg
    10981 atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcctcatgg
    11041 gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca
    11101 ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc
    11161 agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg
    11221 cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc
    11281 gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc
    11341 aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc
    11401 atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg
    11461 cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt
    11521 ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag
    11581 gttttgcgcc attcgatggt gtccgggatc tcgacgctct cccttatgcg actcctgcat
    11641 taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc
    11701 atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc
    11761 gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc
    11821 gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc
    11881 gtagaggatc gagatct
//
