LOCUS Exported 12387 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Level 2 construct. ACCESSION . VERSION . KEYWORDS pUG-314 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12387) AUTHORS Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M TITLE UbiGate: a synthetic biology toolbox to analyse ubiquitination. JOURNAL New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 2017 Nov 30. PUBMED 29194629 REFERENCE 2 (bases 1 to 12387) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12387 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 126..145 /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" primer_bind 229..248 /label=T7 /note="T7 promoter, forward primer" promoter 229..247 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 248..272 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." CDS 326..343 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 3891..3977 /codon_start=1 /product="three tandem HA epitope tags" /label=3xHA /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDY" CDS 3954..3980 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 3993..4079 /codon_start=1 /product="three tandem HA epitope tags" /label=3xHA /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDY" CDS 4056..4082 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" primer_bind complement(7550..7568) /label=T7 Term /note="T7 terminator, reverse primer" terminator 7564..7611 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" rep_origin 7648..8103 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7735..7754) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7945..7966 /label=F1ori-F /note="F1 origin, forward primer" CDS complement(8195..9010) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 8918..8937 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" primer_bind 9002..9021 /label=pENTR-R /note="pENTR vectors, reverse primer" rep_origin 9132..9720 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9621..9640 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9874..9891 /label=L4440 /note="L4440 vector, forward primer" misc_feature 9906..10045 /label=bom /note="basis of mobility region from pBR322" primer_bind complement(9971..9990) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 10131..10153 /label=pGEX 3' /note="pGEX vectors, reverse primer" CDS complement(10147..10338) /codon_start=1 /gene="rop" /product="Rop protein, which maintains plasmids at low copy number" /label=rop /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL" CDS complement(11147..12229) /codon_start=1 /gene="lacI" /product="lac repressor" /label=lacI /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" primer_bind 12191..12210 /label=LacI-R /note="LacI, reverse primer" promoter complement(12230..12307) /gene="lacI" /label=lacI promoter ORIGIN 1 gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct 61 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc 121 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg 181 gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaatta atacgactca 241 ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg ccttaacttt 301 aagaaggaga tataccatgg gcagccacca ccaccaccac cacagcggaa tgcagatctt 361 tgttaagact ctcaccggaa agacaatcac cctagaggtg gaaagctccg acaccatcga 421 caacgttaag gccaagatcc aggataagga gggcattcct ccggatcagc agaggcttat 481 tttcgccggc aagcagctag aggatggccg tacgttggct gattacaata tccagaagga 541 atccaccctc cacttggtcc tcaggctccg tggtggttaa gcttgcaatt aactttaaga 601 aggagatata caatgcttca caagcgagct agtgaagcta acgataagaa cgataatacc 661 atcatcggtt ccgatttagc atcatctaag aaacgccgta tcgatttcac tgaatcttcg 721 tctgataagt catcttcgat tttagcttct ggtagtagca ggggttttca cggcgatagc 781 gtcgttcagc aaatcgacat ggcttttggt aattcgaacc gtcaggagat tgatgaagat 841 ctgcacagtc gccagctcgc cgtctatggt cgtgagacta tgaggcgtct ctttgcttcc 901 aatgttctca tctcggggat gcacggtctt ggtgccgaga ttgccaagaa tcttatactt 961 gctggtgtga agtctgtgac cttgcatgat gaaagagttg tagagctatg ggacttatca 1021 agcaactttg ttttctcgga ggatgatgtt ggcaagaata gagcagatgc ttctgttcag 1081 aagttacagg atcttaacaa tgctgtggtt gtctctagct tgaccaaaag cttaaacaaa 1141 gaggatcttt ctggcttcca ggttgttgta ttctctgaca taagcatgga aagagcaatc 1201 gaatttgatg actattgtca cagccatcag cctcctatcg cttttgtcaa ggctgatgtc 1261 aggggtcttt tcggctctgt cttttgtgat tttgggcctg aatttgcggt tcttgatgtt 1321 gatggagagg aaccccatac aggcatcatt gcgtctatct ctaatgagaa ccaggcattt 1381 atttcctgtg tcgacgatga gaggcttgag tttgaagatg gtgacctagt tgttttctca 1441 gaagttgagg gtatgacaga attgaatgat gggaaaccaa ggaagataaa aagtacacgg 1501 ccatattcat tcacccttga tgaggacact actaactatg gaacatatgt gaagggtggt 1561 attgtcactc aagtgaaaca gccaaagtta ctgaatttca agccattgag ggaagcgctt 1621 aaggatccag gggattttct atttagtgat ttctctaagt ttgatcggcc tccactcctt 1681 cacttagcat tccaggcact tgatcatttt aaagctgaag ctggcagatt tcctgttgct 1741 ggctcagaag aggacgctca gaagcttata tcgattgcca cagccatcaa taccgggcag 1801 ggtgatttaa aggtggagaa tgttgatcag aagcttctaa gacacttttc ctttggagcc 1861 aaggctgtcc tgaatcctat ggctgcaatg tttggcggta ttgttggaca ggaggttgtt 1921 aaagcttgct ctggaaaatt tcatcctctc ttccagtttt tctactttga ttcagtggag 1981 tcactcccct ctgaacctgt ggactcgagt gactttgcac caagaaacag ccggtatgat 2041 gcccaaatat ctgtatttgg ggccaagttc cagaagaaac ttgaagatgc taaagttttc 2101 acagtagggt ctggtgctct tggctgtgag ttcttgaaaa atttggctct gatgggggtt 2161 tcttgtggaa gccaagggaa gctgacagtg acagatgatg atatcattga gaagagtaac 2221 ctcagtcgtc aatttctgtt ccgtgactgg aacattggac aggctaaatc cacagttgct 2281 gcttcggctg ctgctgttat aaatcctcgc ttcaacattg aggccctgca aaaccgtgtt 2341 ggcgcggaga ctgagaatgt atttgacgat gccttctggg agaacttaac tgttgtcgtc 2401 aatgcactgg ataatgttaa tgcgaggctc tatgttgatt cgaggtgctt gtatttccag 2461 aagcctctcc ttgagtctgg aactcttggt acaaagtgca acacacagtc ggttatccca 2521 catctaactg aaaattacgg tgcctcaagg gacccaccag agaaacaggc ccccatgtgt 2581 acagtgcatt ccttcccgca taacattgat cactgtttaa cttgggctcg ctctgagttt 2641 gagggtctgc tggagaaaac tcccgctgaa gtgaatgcat atctttctag cccagttgag 2701 tacactaact caatgatgag tgctggtgat gctcaggcga gggacacatt ggagaggatt 2761 gttgagtgcc ttgaaaagga gaagtgtgaa accttccaag actgcttaac ctgggcacga 2821 ctgaggtttg aggattactt tgtgaaccgt gtcaagcaat tgatatacac atttcctgaa 2881 gatgctgcga ctagtaccgg agctccattc tggtctgctc caaaaagatt ccctcgtcca 2941 ctccagtact cgtcgtccga cccaagtctc cttaacttta tcacagcgac tgctatttta 3001 cgagcagaga catttgggat cccgatacct gagtggacta agaacccaaa ggaagcagca 3061 gaagctgttg acagagtgat agtcccagac tttgagccaa ggcaagatgc aaagattgtg 3121 actgatgaga aagctaccac tttaaccact gcttcggtgg acgacgctgc agtcatcgac 3181 gatctcattg ctaagatcga ccagtgtagg cataacttgt cgccagactt caggatgaaa 3241 cctattcagt tcgaaaagga tgatgataca aactatcaca tggatgtgat agcaggacta 3301 gccaacatga gggcgagaaa ttacagcatc cctgaagtgg acaagctgaa agcaaagttc 3361 atagcaggga gaatcatccc agccattgcg acttcaacag ccatggctac tgggctggtc 3421 tgcctcgagc tgtacaaggt ccttgatgga ggacataaag tggaagctta cagaaacaca 3481 tttgccaacc tggcacttcc actattctcc atggctgaac cacttccacc aaaggtggtg 3541 aagcaccgtg acatggcttg gacagtttgg gacagatggg ttctcaaggg aaacccaact 3601 cttcgtgagg tgttgcagtg gctggaggac aaagggctta gcgcatacag catctcttgt 3661 ggaagctgtc tactgttcaa cagtatgttc acaaggcaca aggagaggat ggacaagaag 3721 gttgtggatc tcgctcggga tgttgcgaaa gtcgagttac cgccttatcg caaccatctt 3781 gatgtagtgg tggcttgtga ggatgaagat gacaatgacg tcgatattcc tctcgtctct 3841 atctacttca ggtgagctta ctattaactt taagaaggag atataccatg tacccatacg 3901 atgttcctga ctatgcgggc tatccctatg acgtcccgga ctatgcagga ttgtatccat 3961 atgacgttcc agattacgcc actagagctg cttacccata cgatgttcct gactatgcgg 4021 gctatcccta tgacgtcccg gactatgcag gattgtatcc atatgacgtt ccagattacg 4081 caatggagca tgagcaagat gaccctggta catcaacaaa tgtaggggtt gactcgtcag 4141 ttgatgattc aatggcaagt ctctctatat gtgattcaga gcatcctaat atttatcgac 4201 aagatattgt caaaaacaaa aagaccggta gtgttggtgt tgtcagcgaa gtagctggtg 4261 attctgactc tgatagtgat attagtgatg aggaagaaga tgatgatgat gacgaagata 4321 atgatgatga tgatgaggat gtagaggaag ggaaaaaggc tagtgaagaa aatgttgtta 4381 atggagatgg cgagaaaaaa gctgatggga attataaatg tggtgcactt gagggagatc 4441 agattcgtgt gctttggatg gataacactg agcctgttca agatattaat gatgtaactg 4501 ttatagaccg tggtttctta catggtgatt atgttgcttc tgcttctgag cctactggtc 4561 aggtgggagt tgttgtggat gttaacatat cagttgattt gttggctcct gatggttcta 4621 ttcacaagga catctctacc aagaatttga aacgcgtcag ggattttgct gttggtgatt 4681 atgtggttca tggtccatgg ctaggtagaa tcgatgatgt gttggacaat gtgactgtct 4741 tatttgatga tggttccatg tgtaaagttc tacgtgttga acctcttcga cttaaaccta 4801 ttccgaagaa taatcttgaa gaagatgcta attttcctta ttacccaggg cagcgggtta 4861 aagcaagctc ctcttcggtt tttaagaatt cgcggtggtt atctggactg tggaagccta 4921 atcgtttaga aggtacggtg accaaagtta ctgcgggatc aatttttgtc tactggattg 4981 cctcagctgg ctttggtcca gattcttctg tttccccacc tgaggagcag aatccaagta 5041 atttgacatt gttgtcatgt ttcactcatg ccaattggca agtgggtgac tggtgccttc 5101 ttccatctct gaatcaatct gctacaatcc ccttacacaa gcatgtatct aaattaagac 5161 tttatgattc ccaagctgat cggcaacaga aaattgggcg tgatttagaa gatgtgcaag 5221 atgaagtaag tggaaaagtt gaaccagctg gtattactgc tgaagctttg ccaaaagtaa 5281 ctagtgacga tcctcctcaa agaaatcctt ctgtttcaaa ggagcctgtt catgagcctt 5341 ggcctctcca tcggaagaag attcgcaaac ttgttatcag aaaggacaaa aaggttaaga 5401 aaaaagagga aagctttgaa caagctcttt tggtagttaa tagcagaaca cgcgttgatg 5461 tatcctggca ggatggtaca atagaatgta gacgggaagc gataacattg attccaattg 5521 agactcctgg tgatcatgaa tttgtctctg agcagtatgt ggtagagagg acctctgatg 5581 atggtgataa cacaactgaa cctagacgtg ctggcgttgt aaaaaatgtc aatgcaaaag 5641 atcgtactgc ttctgtgagg tggctaaatc cacttcgaag ggcagaagaa ccccgtgagt 5701 ttgaaaagga agaaattgtc agtgtgtatg agctagaggg ccatccagat tatgactact 5761 gttatgggga tgttgttgtt cgcttatcac ctattgccgt tgcgttacca gcatcttctc 5821 ccggaaactc ttttgaggag gcaacacagc aagacaatgg ataccaagat tcagaatccc 5881 atcaagaagc taaaatcctt gtggataagg aagaaaacga gcctagtacg gacctttcaa 5941 agctctcatg ggttggaaat attactggcc tcaaggatgg tgatattgaa gtaacatggg 6001 ccgatggaac aatatcaacg gttggccctc atgcagttta tgtagttgga cgcgatgatg 6061 atgatgaatc agttgctgga gaaagtgaaa caagtgatgc tgctagttgg gaaactttaa 6121 acgatgatga taggggtgct cctgagattc ctgaagagga ccttggaagg agtagttcca 6181 ttgagggaaa ctctgatgca gatatctacg ctgagaatga ttctgggagg aacggtgccc 6241 tagctcttcc tctggctgca atcgaatttg tgactcgact ggctagtgga atcttttcgc 6301 gtgcaaggaa atctgtagat tcttccagtt ctgattacac ggttgaaaat gtatataagc 6361 aagctgaatc gaccaacccg tccgatgaaa cagattccct tgatgaccct agtccttcaa 6421 aagttaatgt gactgataat tgcgagtcaa agggcactca agcaaatgcc aagaatattt 6481 tgagcgggga aacctctaca tttttagaag atgaagataa gccagtacct tctgaaggtg 6541 atagttgcag tttcagacgc tttgatatat cacaagatcc tctggaccac catttccttg 6601 gcgtagatgg gcagaaaacc aaagaaagac agtggttcaa gaaggttgat caagactgga 6661 aaatacttca gaacaatctt ccagatggaa tctttgttcg agcttacgaa gatagaatgg 6721 atctcttgag ggcagtaata gtcggggcat ttggaacacc ataccaagat ggactcttct 6781 tttttgattt tcaccttcca tctgactacc ctagtgtgcc accgtcggca tattatcatt 6841 ctggtggatg gaggttaaac cccaatctgt acgaagaagg caaagtctgt cttagccttc 6901 tcaatacctg gacaggcagg ggaaatgaag tctgggatcc caaatcatct agcatccttc 6961 aagttcttgt ttcactccag ggattggtgt tgaattcaaa gccttatttc aatgaagccg 7021 ggtatgataa gcaggtcgga actgcggaag gagaaaaaaa ctcactggga tacaacgaga 7081 atactttctt gctaaattgt aaaaccatga tgtatcttat gcggaaacca ccaaaggatt 7141 ttgaagaact catcaaagat catttcagaa aacgcggata ttacattttg aaggcatgtg 7201 acgcctacat gaaaggatat ctcataggtt ccctcaccaa agatgcctcg gttatcgatg 7261 aacgcagcag cgctaattca acttcagttg gttttaagct tatgctggct aagatagcac 7321 caaagctttt ctcagcactg agcgaggtag gagctgactg caatgaattc cagcatctgc 7381 agcagcaata agcttttacg cttttaagcc agccccgaca cccgccaaca cccgctgacg 7441 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgggatga 7501 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa 7561 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga 7621 ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg 7681 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt 7741 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc 7801 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg 7861 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga 7921 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc 7981 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa 8041 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa 8101 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat 8161 acattcaaat atgtatccgc tcatgaatta attcttagaa aaactcatcg agcatcaaat 8221 gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa agccgtttct 8281 gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt 8341 ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa 8401 ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt 8461 tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac 8521 tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagtcga aatacgcgat 8581 cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca 8641 gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt 8701 tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga 8761 tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat 8821 cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat 8881 acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat 8941 ataaatcagc atccatgttg gaatttaatc gcggcctaga gcaagacgtt tcccgttgaa 9001 tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg 9061 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 9121 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 9181 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 9241 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta 9301 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 9361 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 9421 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 9481 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 9541 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 9601 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 9661 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 9721 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 9781 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct 9841 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa 9901 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt 9961 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc 10021 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg 10081 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgact ccgggagctg 10141 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc 10201 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt 10261 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt 10321 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat 10381 gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg 10441 gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa 10501 aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag 10561 ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt 10621 ttccagactt tacgaaacac ggaaacccaa gaccattcat gttgttgctc aggtcgcaga 10681 cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc 10741 agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac 10801 ccgtggggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg 10861 accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc 10921 gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg 10981 cacctgtcct acgagttgca tgataaagaa cacagtcata agtgcggcga cgatagtcat 11041 gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga 11101 tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt 11161 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 11221 cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgacacg ggcaacagct 11281 gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc 11341 ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtcct 11401 cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg gactcggtaa 11461 tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga 11521 tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt 11581 cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac 11641 gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca 11701 atgcgaccag atgctccacg cccagtcgcg taccgtcctc atgggagaaa ataatactgt 11761 tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt 11821 ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt 11881 gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg 11941 acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg 12001 acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg 12061 ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt 12121 tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat 12181 aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc 12241 tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga 12301 tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt 12361 agtaggttga ggccgttgag caccgcc //