LOCUS Exported 10908 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Level 2 construct. ACCESSION . VERSION . KEYWORDS pUG-316 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10908) AUTHORS Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M TITLE UbiGate: a synthetic biology toolbox to analyse ubiquitination. JOURNAL New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 2017 Nov 30. PUBMED 29194629 REFERENCE 2 (bases 1 to 10908) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10908 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 584..603 /label=LacI-R /note="LacI, reverse primer" promoter complement(623..700) /gene="lacI" /label=lacI promoter primer_bind 906..925 /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" primer_bind 1009..1028 /label=T7 /note="T7 promoter, forward primer" promoter 1009..1027 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 1028..1052 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." CDS 1106..1123 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 4671..4757 /codon_start=1 /product="three tandem HA epitope tags" /label=3xHA /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDY" CDS 4734..4760 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 4773..4859 /codon_start=1 /product="three tandem HA epitope tags" /label=3xHA /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDY" CDS 4836..4862 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" primer_bind complement(6851..6869) /label=T7 Term /note="T7 terminator, reverse primer" terminator 6865..6912 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" rep_origin 6949..7404 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7036..7055) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7246..7267 /label=F1ori-F /note="F1 origin, forward primer" CDS complement(7496..8311) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 8219..8238 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" primer_bind 8303..8322 /label=pENTR-R /note="pENTR vectors, reverse primer" rep_origin 8433..9021 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8922..8941 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9175..9192 /label=L4440 /note="L4440 vector, forward primer" misc_feature 9207..9346 /label=bom /note="basis of mobility region from pBR322" primer_bind complement(9272..9291) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 9432..9454 /label=pGEX 3' /note="pGEX vectors, reverse primer" CDS complement(9448..9639) /codon_start=1 /gene="rop" /product="Rop protein, which maintains plasmids at low copy number" /label=rop /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL" CDS complement(10448..622) /codon_start=1 /gene="lacI" /product="lac repressor" /label=lacI /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" ORIGIN 1 tgcgagtgag atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg 61 ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc acgcccagtc 121 gcgtaccgtc ctcatgggag aaaataatac tgttgatggg tgtctggtca gagacatcaa 181 gaaataacgc cggaacatta gtgcaggcag cttccacagc aatggcatcc tggtcatcca 241 gcggatagtt aatgatcagc ccactgacgc gttgcgcgag aagattgtgc accgccgctt 301 tacaggcttc gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat 361 cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagg 421 tggcaacgcc aatcagcaac gactgtttgc ccgccagttg ttgtgccacg cggttgggaa 481 tgtaattcag ctccgccatc gccgcttcca ctttttcccg cgttttcgca gaaacgtggc 541 tggcctggtt caccacgcgg gaaacggtct gataagagac accggcatac tctgcgacat 601 cgtataacgt tactggtttc acattcacca ccctgaattg actctcttcc gggcgctatc 661 atgccatacc gcgaaaggtt ttgcgccatt cgatggtgtc cgggatctcg acgctctccc 721 ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc 781 gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct 841 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc 901 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg 961 gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaatta atacgactca 1021 ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg ccttaacttt 1081 aagaaggaga tataccatgg gcagccacca ccaccaccac cacagcggaa tgcagatctt 1141 tgttaagact ctcaccggaa agacaatcac cctagaggtg gaaagctccg acaccatcga 1201 caacgttaag gccaagatcc aggataagga gggcattcct ccggatcagc agaggcttat 1261 tttcgccggc aagcagctag aggatggccg tacgttggct gattacaata tccagaagga 1321 atccaccctc cacttggtcc tcaggctccg tggtggttaa gcttgcaatt aactttaaga 1381 aggagatata caatgcttca caagcgagct agtgaagcta acgataagaa cgataatacc 1441 atcatcggtt ccgatttagc atcatctaag aaacgccgta tcgatttcac tgaatcttcg 1501 tctgataagt catcttcgat tttagcttct ggtagtagca ggggttttca cggcgatagc 1561 gtcgttcagc aaatcgacat ggcttttggt aattcgaacc gtcaggagat tgatgaagat 1621 ctgcacagtc gccagctcgc cgtctatggt cgtgagacta tgaggcgtct ctttgcttcc 1681 aatgttctca tctcggggat gcacggtctt ggtgccgaga ttgccaagaa tcttatactt 1741 gctggtgtga agtctgtgac cttgcatgat gaaagagttg tagagctatg ggacttatca 1801 agcaactttg ttttctcgga ggatgatgtt ggcaagaata gagcagatgc ttctgttcag 1861 aagttacagg atcttaacaa tgctgtggtt gtctctagct tgaccaaaag cttaaacaaa 1921 gaggatcttt ctggcttcca ggttgttgta ttctctgaca taagcatgga aagagcaatc 1981 gaatttgatg actattgtca cagccatcag cctcctatcg cttttgtcaa ggctgatgtc 2041 aggggtcttt tcggctctgt cttttgtgat tttgggcctg aatttgcggt tcttgatgtt 2101 gatggagagg aaccccatac aggcatcatt gcgtctatct ctaatgagaa ccaggcattt 2161 atttcctgtg tcgacgatga gaggcttgag tttgaagatg gtgacctagt tgttttctca 2221 gaagttgagg gtatgacaga attgaatgat gggaaaccaa ggaagataaa aagtacacgg 2281 ccatattcat tcacccttga tgaggacact actaactatg gaacatatgt gaagggtggt 2341 attgtcactc aagtgaaaca gccaaagtta ctgaatttca agccattgag ggaagcgctt 2401 aaggatccag gggattttct atttagtgat ttctctaagt ttgatcggcc tccactcctt 2461 cacttagcat tccaggcact tgatcatttt aaagctgaag ctggcagatt tcctgttgct 2521 ggctcagaag aggacgctca gaagcttata tcgattgcca cagccatcaa taccgggcag 2581 ggtgatttaa aggtggagaa tgttgatcag aagcttctaa gacacttttc ctttggagcc 2641 aaggctgtcc tgaatcctat ggctgcaatg tttggcggta ttgttggaca ggaggttgtt 2701 aaagcttgct ctggaaaatt tcatcctctc ttccagtttt tctactttga ttcagtggag 2761 tcactcccct ctgaacctgt ggactcgagt gactttgcac caagaaacag ccggtatgat 2821 gcccaaatat ctgtatttgg ggccaagttc cagaagaaac ttgaagatgc taaagttttc 2881 acagtagggt ctggtgctct tggctgtgag ttcttgaaaa atttggctct gatgggggtt 2941 tcttgtggaa gccaagggaa gctgacagtg acagatgatg atatcattga gaagagtaac 3001 ctcagtcgtc aatttctgtt ccgtgactgg aacattggac aggctaaatc cacagttgct 3061 gcttcggctg ctgctgttat aaatcctcgc ttcaacattg aggccctgca aaaccgtgtt 3121 ggcgcggaga ctgagaatgt atttgacgat gccttctggg agaacttaac tgttgtcgtc 3181 aatgcactgg ataatgttaa tgcgaggctc tatgttgatt cgaggtgctt gtatttccag 3241 aagcctctcc ttgagtctgg aactcttggt acaaagtgca acacacagtc ggttatccca 3301 catctaactg aaaattacgg tgcctcaagg gacccaccag agaaacaggc ccccatgtgt 3361 acagtgcatt ccttcccgca taacattgat cactgtttaa cttgggctcg ctctgagttt 3421 gagggtctgc tggagaaaac tcccgctgaa gtgaatgcat atctttctag cccagttgag 3481 tacactaact caatgatgag tgctggtgat gctcaggcga gggacacatt ggagaggatt 3541 gttgagtgcc ttgaaaagga gaagtgtgaa accttccaag actgcttaac ctgggcacga 3601 ctgaggtttg aggattactt tgtgaaccgt gtcaagcaat tgatatacac atttcctgaa 3661 gatgctgcga ctagtaccgg agctccattc tggtctgctc caaaaagatt ccctcgtcca 3721 ctccagtact cgtcgtccga cccaagtctc cttaacttta tcacagcgac tgctatttta 3781 cgagcagaga catttgggat cccgatacct gagtggacta agaacccaaa ggaagcagca 3841 gaagctgttg acagagtgat agtcccagac tttgagccaa ggcaagatgc aaagattgtg 3901 actgatgaga aagctaccac tttaaccact gcttcggtgg acgacgctgc agtcatcgac 3961 gatctcattg ctaagatcga ccagtgtagg cataacttgt cgccagactt caggatgaaa 4021 cctattcagt tcgaaaagga tgatgataca aactatcaca tggatgtgat agcaggacta 4081 gccaacatga gggcgagaaa ttacagcatc cctgaagtgg acaagctgaa agcaaagttc 4141 atagcaggga gaatcatccc agccattgcg acttcaacag ccatggctac tgggctggtc 4201 tgcctcgagc tgtacaaggt ccttgatgga ggacataaag tggaagctta cagaaacaca 4261 tttgccaacc tggcacttcc actattctcc atggctgaac cacttccacc aaaggtggtg 4321 aagcaccgtg acatggcttg gacagtttgg gacagatggg ttctcaaggg aaacccaact 4381 cttcgtgagg tgttgcagtg gctggaggac aaagggctta gcgcatacag catctcttgt 4441 ggaagctgtc tactgttcaa cagtatgttc acaaggcaca aggagaggat ggacaagaag 4501 gttgtggatc tcgctcggga tgttgcgaaa gtcgagttac cgccttatcg caaccatctt 4561 gatgtagtgg tggcttgtga ggatgaagat gacaatgacg tcgatattcc tctcgtctct 4621 atctacttca ggtgagctta ctattaactt taagaaggag atataccatg tacccatacg 4681 atgttcctga ctatgcgggc tatccctatg acgtcccgga ctatgcagga ttgtatccat 4741 atgacgttcc agattacgcc actagagctg cttacccata cgatgttcct gactatgcgg 4801 gctatcccta tgacgtcccg gactatgcag gattgtatcc atatgacgtt ccagattacg 4861 caatggagcc caacgtagtg gagatcgcta ctcctcctgc cgcttcttgc tcgagaattc 4921 gaacgccaac aaaggctgaa actccagaag tgattgatgt ggaggaatat gatttgcaaa 4981 acggtggtgt tcctaatggc aacaatgttg attataagaa caaagggaag gctatagact 5041 ttgattccat gtcttacggt gactatggtg aagaagatga gtatgctgtg ggatctcctg 5101 gtgatgatta tggttatcct gaatcatctc cactatcaaa tagtttgtta gacccagagt 5161 ctttgatcta tgaggacgac gaaaactatt ccgaacaata tgactttgaa atggaggcgg 5221 agcctgataa ttactccatg tatcaagacc ttttcgatgg taaggatatc ccaactggtg 5281 tggaggtttc aatggattgg tttccaaatt cagctgacaa ggaatcagcg agtagtagca 5341 aatcgtctca tgctaataac gggaataaca gttccaagaa agcaacaaaa gcaagtggaa 5401 tccattctca attttcttca gatatggaaa cccctgtagc tcaaccatgg aatgctttac 5461 cgcacaaagc tgaaggagtc ataccaaact ctgcttatgc cttgccacag aacagcaagg 5521 cttttcaacc accttacgct gtccactatt cagcccttaa aactgctttt tccaattatc 5581 ttcagcctca gactcctgat accgtattgg gggaggctcc agctcctgct gcaggttctt 5641 ctggtttatt gccaccaaac acacccggtt ttaaaagtaa tgctgctcgg tttaaagagg 5701 agccgcctat attacctccg gatgattcca gagttaagag aaatatggag gattatcttg 5761 ggctttattt gtttttcaaa agatttgaca tcgtcgaaga tttttcagat caccactatg 5821 cttctaaggg aacaacttca aagcagcatt caaaggattg ggcgaaaagg atccaagacg 5881 agtggagaat tcttgagaaa gatttaccag agatgatatt tgtcagagct tatgaatcta 5941 ggatggatct tttaagggct gtaattatcg gagcccaagg aactccctac catgatggac 6001 tcttcttttt tgatatcttc ttcccggata catacccttc tacgccaccg attgttcatt 6061 accattctgg tgggctaaga atcaacccaa atctgtacaa ttgtgggaaa gtgtgtctga 6121 gtctactcgg cacttggagt ggtaaccaga gggagaaatg gatccccaac acttcgacga 6181 tgctacaagt tcttgtctcg atccaaggtc taatcttaaa tcaaaaacct tacttcaacg 6241 agcccggcta cgagaggtca gcaggttctg cacatggtga gagtacctct aaagcttaca 6301 gcgaaaacac attcatactg tctttaaaga ctatggttta caccatgagg aggccaccca 6361 agtattttga ggactttgcg tatggacatt tcttcagctg tgctcacgac gtgttgaagg 6421 cgtgtaatgc ttacagaaac ggagctacac cgggatatct ggtgaagggg gctccagatg 6481 tggaagagaa tagcgcgggc atgagctctc tgaagtttag gacagatgtg gctacctttg 6541 tggagacagt gctgttgaaa gagtttattc ttctaggtgt ccttggactc gaaccggaag 6601 aagaagaaaa aacacctgag actataatcg ttgcagagag tagcaaatgt accagatcaa 6661 ggtccaagag agacagagtt tcttctagtt gagcttttac gcttttaagc cagccccgac 6721 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca 6781 gacaagctgt gaccgggatg agatccggct gctaacaaag cccgaaagga agctgagttg 6841 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg 6901 aggggttttt tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta 6961 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca 7021 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct 7081 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc 7141 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat 7201 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc 7261 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc 7321 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta 7381 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc 7441 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgaatt aattcttaga 7501 aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat 7561 atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga 7621 tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta 7681 atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat 7741 ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca ggccagccat 7801 tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct 7861 gagcgagtcg aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca 7921 accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt 7981 ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag 8041 gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc 8101 tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact 8161 ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat 8221 cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctag 8281 agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag 8341 cagacagttt tattgttcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg 8401 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 8461 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 8521 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc 8581 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 8641 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 8701 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 8761 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 8821 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 8881 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 8941 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 9001 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 9061 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt 9121 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag 9181 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc 9241 ggtatttcac accgcaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa 9301 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc 9361 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc 9421 tgtgaccgac tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc 9481 gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg 9541 ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa 9601 gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg 9661 atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt 9721 tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg 9781 gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga 9841 tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt 9901 gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccca agaccattca 9961 tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat 10021 cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga 10081 caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc 10141 gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc 10201 gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa 10261 aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga acacagtcat 10321 aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc 10381 tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc 10441 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 10501 cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca 10561 ccagtgacac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca 10621 agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg 10681 ggatataaca tgagctgtcc tcggtatcgt cgtatcccac taccgagata tccgcaccaa 10741 cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa 10801 ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg 10861 acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgat //