LOCUS Exported 9662 bp ds-DNA linear SYN 15-JUL-2021 DEFINITION Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I576V approximately wildtype transport. ACCESSION . VERSION . KEYWORDS pCEP4-SERTI576V-myc SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9662) TITLE Procko Lab SERT constructs REFERENCE 2 (bases 1 to 9662) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 15, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9662 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(719..737) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 805..909 /gene="bla" /label=AmpR promoter CDS 910..1770 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1128..1147) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1941..2529 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2430..2449 /label=pBR322ori-F /note="pBR322 origin, forward primer" promoter 2960..3105 /label=HSV TK promoter /note="herpes simplex virus thymidine kinase promoter" CDS 3148..4185 /codon_start=1 /product="hygromycin B phosphotransferase" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE MGEAN" primer_bind complement(4183..4202) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4227..4275 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" enhancer 4929..5308 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 5309..5512 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 5463..5483 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 5509..5533 /label=LNCX /note="Human CMV promoter, forward primer" regulatory 5561..5570 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 6236..6265 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" polyA_signal 7554..7688 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7578..7597) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 7632..7651 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(7831..7853) /label=pGEX 3' /note="pGEX vectors, reverse primer" ORIGIN 1 gctcctgctc ctgctcctgt tccaccgtgg gtccctttgc agccaatgca acttggacgt 61 ttttggggtc tccggacacc atctctatgt cttggccctg atcctgagcc gcccggggct 121 cctggtcttc cgcctcctcg tcctcgtcct cttccccgtc ctcgtccatg gttatcaccc 181 cctcttcttt gaggtccact gccgccggag ccttctggtc cagatgtgtc tcccttctct 241 cctaggccat ttccaggtcc tgtacctggc ccctcgtcag acatgattca cactaaaaga 301 gatcaataga catctttatt agacgacgct cagtgaatac agggagtgca gactcctgcc 361 ccctccaaca gcccccccac cctcatcccc ttcatggtcg ctgtcagaca gatccaggtc 421 tgaaaattcc ccatcctccg aaccatcctc gtcctcatca ccaattactc gcagcccgga 481 aaactcccgc tgaacatcct caagatttgc gtcctgagcc tcaagccagg cctcaaattc 541 ctcgtccccc tttttgctgg acggtaggga tggggattct cgggacccct cctcttcctc 601 ttcaaggtca ccagacagag atgctactgg ggcaacggaa gaaaagctgg gtgcggcctg 661 tgaggatcag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc 721 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca 781 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 841 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 901 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 961 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 1021 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 1081 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 1141 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 1201 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 1261 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 1321 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 1381 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 1441 accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 1501 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 1561 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 1621 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 1681 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 1741 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 1801 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 1861 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 1921 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 1981 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 2041 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag 2101 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 2161 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 2221 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 2281 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 2341 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 2401 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 2461 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 2521 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggccttga 2581 agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc 2641 ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg 2701 aacgccagca agacgtagcc cagcgcgtcg gccccgagat gcgccgcgtg cggctgctgg 2761 agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc 2821 gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccctg 2881 cttcatcccc gtggcccgtt gctcgcgttt gctggcggtg tccccggaag aaatatattt 2941 gcatgtcttt agttctatga tgacacaaac cccgcccagc gtcttgtcat tggcgaattc 3001 gaacacgcag atgcagtcgg ggcggcgcgg tccgaggtcc acttcgcata ttaaggtgac 3061 gcgtgtggcc tcgaacaccg agcgaccctg cagcgacccg cttaacagcg tcaacagcgt 3121 gccgcagatc ccggggggca atgagatatg aaaaagcctg aactcaccgc gacgtctgtc 3181 gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc 3241 gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat 3301 agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg 3361 ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc 3421 tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt 3481 ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc 3541 gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata 3601 tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt 3661 gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc 3721 cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata 3781 acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac 3841 atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg 3901 aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt 3961 gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt 4021 cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc 4081 agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga 4141 cgccccagca ctcgtccgga tcgggagatg ggggaggcta actgaaacac ggaaggagac 4201 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacgggtg 4261 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc 4321 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc 4381 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag 4441 ccactggccc cgtgggttag ggacggggtc ccccatgggg aatggtttat ggttcgtggg 4501 ggttattatt ttgggcgttg cgtggggtca ggtccacgac tggactgagc agacagaccc 4561 atggtttttg gatggcctgg gcatggaccg catgtactgg cgcgacacga acaccgggcg 4621 tctgtggctg ccaaacaccc ccgaccccca aaaaccaccg cgcggatttc tggcgtgcca 4681 agctagtcga ccaattctca tgtttgacag cttatcatcg cagatccggg caacgttgtt 4741 gccattgctg caggcgcaga actggtaggt atggaagatc tatacattga atcaatattg 4801 gcaattagcc atattagtca ttggttatat agcataaatc aatattggct attggccatt 4861 gcatacgttg tatctatatc ataatatgta catttatatt ggctcatgtc caatatgacc 4921 gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt 4981 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 5041 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 5101 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc 5161 agtacatcaa gtgtatcata tgccaagtcc gccccctatt gacgtcaatg acggtaaatg 5221 gcccgcctgg cattatgccc agtacatgac cttacgggac tttcctactt ggcagtacat 5281 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca ccaatgggcg 5341 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag 5401 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaataacc ccgccccgtt 5461 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt 5521 gaaccgtcag atctctagaa gctgggtacc agctgctagc gccaccatgg agaccacccc 5581 cctgaacagt caaaagcaac tctctgcctg tgaggatgga gaggactgtc aggagaatgg 5641 cgttcttcaa aaggttgtac ccacccctgg agataaggtg gaaagcggcc agatttccaa 5701 cggctacagc gccgtgccat cacctggagc cggggatgat acacggcaca gcattcccgc 5761 aaccaccacg acactggttg cagagcttca ccagggcgag agggagacgt ggggcaaaaa 5821 ggtagatttt ctgctttccg tgatcggtta cgcggtcgat ctggggaacg tgtggaggtt 5881 cccttacatt tgttaccaaa acggaggggg tgccttcttg ctgccctaca caattatggc 5941 gatttttggg ggtatcccgc tcttctacat ggaactggcc ttgggtcagt accaccggaa 6001 cggctgcatt agcatttggc gcaagatatg ccccattttc aagggaatcg gatacgcaat 6061 ctgtataatc gcgttctata ttgcatcata ttacaacacc atcatggcct gggcccttta 6121 ttacttgatc agcagtttca ccgaccaatt gccgtggacg agctgtaaga actcatggaa 6181 taccgggaac tgtaccaact acttctctga ggacagctca ggaggtagcg ggggtgaaca 6241 gaagctgatt agcgaggagg acttgggcag tccaggcgga agcggcaaca tcacttggac 6301 actccatagt acgagccccg cagaggagtt ttacaccagg cacgtactgc agattcatag 6361 gagcaagggt ctgcaagacc tgggcggtat tagctggcag ctcgcattgt gcataatgtt 6421 gatctttacg gtgatctatt tctctatctg gaagggagta aagacatccg gcaaggtggt 6481 gtgggtgacg gcgaccttcc cctacattat actcagcgtg ttgttggtga ggggggcaac 6541 cctgcctggg gcctggcgag gcgtgctgtt ctatctcaag ccaaactggc agaagcttct 6601 tgaaaccgga gtgtggatcg acgcggccgc gcaaatcttc ttttccctcg gtcccggctt 6661 cggggtcctt ctggcattcg caagctacaa caagttcaac aataactgct accaggacgc 6721 cctcgtgaca agtgtagtga actgcatgac ctctttcgtg agcgggtttg tgatcttcac 6781 agtactcgga tatatggctg agatgaggaa cgaagatgtt agtgaggttg ccaaagacgc 6841 tggcccgagc ctgttgttca tcacatacgc cgaggctatc gcgaacatgc ccgcatctac 6901 tttcttcgcc atcatctttt tcctgatgct gataaccctt gggctggata gcaccttcgc 6961 cggactggag ggtgtcatca ctgccgttct cgacgagttc ccccatgtgt gggccaagcg 7021 acgggaaagg ttcgtgctgg ccgtcgtaat cacatgcttc ttcggctcac tggtgaccct 7081 tacctttggc ggtgcgtacg tggtcaagct gctcgaagag tacgccactg gaccggcggt 7141 tctgaccgtg gcattgatcg aggccgtggc cgtaagctgg ttttacggca tcacgcagtt 7201 ctgtagggac gtgaaagaga tgctgggctt ttctcccggt tggttttgga ggatttgttg 7261 ggtagcaatt agccccctgt ttctcctttt cattatttgc tcttttctga tgtctccacc 7321 gcagctccga ctgtttcagt acaactaccc ctactggtca attgtcctgg gctattgtat 7381 cggcacctca agtttcatct gcatccccac atacatcgca tacagactga taattacgcc 7441 cggtactttt aaggagcgaa tcattaaaag cataaccccc gaaaccccca ccgagatccc 7501 ttgtggcgat ataagactca atgcggtcta atagctcgag gccggcaagg ccggatccag 7561 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat 7621 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata 7681 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg 7741 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatccggctg 7801 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt 7861 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg 7921 tgttggcggg tgtcggggcg cagccatgag gtcgactcta gaggatcgat ccccgccccg 7981 gacgaactaa acctgactac gacatctctg ccccttcttc gcggggcagt gcatgtaatc 8041 ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga cacggggggg 8101 gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg atgtgcacat 8161 ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg actgcaacac 8221 aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg ggggacatgt 8281 acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca gaggggcctg 8341 tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta taaggccccc 8401 ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact atccagacta 8461 accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa ttagggttag 8521 taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg gttttattta 8581 catggggtca ggattccacg agggtagtga accattttag tcacaagggc agtggctgaa 8641 gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat tctccttcgt 8701 ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa 8761 caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt ggtggcattg 8821 tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta gtgtaggaat 8881 gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag ccgtaaagac 8941 tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta gtgcaatatg 9001 atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac catgttgtta 9061 cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac tccactggtt 9121 gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca taaacaaaga 9181 caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc 9241 cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt gtaaccccgc 9301 taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg gggaatacct 9361 gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg aggacaaatt 9421 acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt cacgaggtcg 9481 ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa tatctggata 9541 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta 9601 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta 9661 gc //