LOCUS Exported 12149 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I576V approximately wildtype transport. ACCESSION . VERSION . KEYWORDS pCEP4-SERTI576V-myc SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12149) TITLE Procko Lab SERT constructs REFERENCE 2 (bases 1 to 12149) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12149 /organism="synthetic DNA construct" /mol_type="other DNA" polyA_signal 14..148 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(38..57) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 92..111 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(291..313) /label=pGEX 3' /note="pGEX vectors, reverse primer" rep_origin 873..2662 /label=oriP /note="Epstein-Barr virus oriP replication origin (Yates et al., 2000)" CDS complement(2968..4893) /codon_start=1 /product="Epstein-Barr nuclear antigen 1, also known as EBNA-1" /label=EBNA1 /note="crucial for latent viral infection, and for episomal amplification of vectors containing the oriP origin (Okita et al., 2013)" /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE GEEGQE" primer_bind complement(5328..5346) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 5414..5518 /gene="bla" /label=AmpR promoter CDS 5519..6379 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5737..5756) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6550..7138 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7039..7058 /label=pBR322ori-F /note="pBR322 origin, forward primer" promoter 7569..7714 /label=HSV TK promoter /note="herpes simplex virus thymidine kinase promoter" CDS 7757..8794 /codon_start=1 /product="hygromycin B phosphotransferase" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE MGEAN" primer_bind complement(8792..8811) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 8836..8884 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" enhancer 9538..9917 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 9918..10121 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 10072..10092 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 10118..10142 /label=LNCX /note="Human CMV promoter, forward primer" regulatory 10170..10179 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 10845..10874 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" ORIGIN 1 gccggcaagg ccggatccag acatgataag atacattgat gagtttggac aaaccacaac 61 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt 121 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca 181 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat 241 ggctgattat gatccggctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac 301 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc 361 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgag gtcgactcta 421 gaggatcgat ccccgccccg gacgaactaa acctgactac gacatctctg ccccttcttc 481 gcggggcagt gcatgtaatc ccttcagttg gttggtacaa cttgccaact gggccctgtt 541 ccacatgtga cacggggggg gaccaaacac aaaggggttc tctgactgta gttgacatcc 601 ttataaatgg atgtgcacat ttgccaacac tgagtggctt tcatcctgga gcagactttg 661 cagtctgtgg actgcaacac aacattgcct ttatgtgtaa ctcttggctg aagctcttac 721 accaatgctg ggggacatgt acctcccagg ggcccaggaa gactacggga ggctacacca 781 acgtcaatca gaggggcctg tgtagctacc gataagcgga ccctcaagag ggcattagca 841 atagtgttta taaggccccc ttgttaaccc taaacgggta gcatatgctt cccgggtagt 901 agtatatact atccagacta accctaattc aatagcatat gttacccaac gggaagcata 961 tgctatcgaa ttagggttag taaaagggtc ctaaggaaca gcgatatctc ccaccccatg 1021 agctgtcacg gttttattta catggggtca ggattccacg agggtagtga accattttag 1081 tcacaagggc agtggctgaa gatcaaggag cgggcagtga actctcctga atcttcgcct 1141 gcttcttcat tctccttcgt ttagctaata gaataactgc tgagttgtga acagtaaggt 1201 gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa atttggacgg 1261 ggggttcagt ggtggcattg tgctatgaca ccaatataac cctcacaaac cccttgggca 1321 ataaatacta gtgtaggaat gaaacattct gaatatcttt aacaatagaa atccatgggg 1381 tggggacaag ccgtaaagac tggatgtcca tctcacacga atttatggct atgggcaaca 1441 cataatccta gtgcaatatg atactggggt tattaagatg tgtcccaggc agggaccaag 1501 acaggtgaac catgttgtta cactctattt gtaacaaggg gaaagagagt ggacgccgac 1561 agcagcggac tccactggtt gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca 1621 atggggccca taaacaaaga caagtggcca ctcttttttt tgaaattgtg gagtgggggc 1681 acgcgtcagc ccccacacgc cgccctgcgg ttttggactg taaaataagg gtgtaataac 1741 ttggctgatt gtaaccccgc taaccactgc ggtcaaacca cttgcccaca aaaccactaa 1801 tggcaccccg gggaatacct gcataagtag gtgggcgggc caagataggg gcgcgattgc 1861 tgcgatctgg aggacaaatt acacacactt gcgcctgagc gccaagcaca gggttgttgg 1921 tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg ggtagcatat 1981 actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta gcataggcta 2041 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta 2101 tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta 2161 tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta 2221 tcctaataga gattagggta gtatatgcta tcctaattta tatctgggta gcatatacta 2281 cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat atgctatcct 2341 aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct 2401 aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct 2461 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct 2521 aatctgtatc cgggtagcat atgctatcct catgcatata cagtcagcat atgataccca 2581 gtagtagagt gggagtgcta tcctttgcat atgccgccac ctcccaaggg ggcgtgaatt 2641 ttcgctgctt gtccttttcc tgcatgctgg ttgctcccat tcttaggtga atttaaggag 2701 gccaggctaa agccgtcgca tgtctgattg ctcaccaggt aaatgtcgct aatgttttcc 2761 aacgcgagaa ggtgttgagc gcggagctga gtgacgtgac aacatgggta tgcccaattg 2821 ccccatgttg ggaggacgaa aatggtgaca agacagatgg ccagaaatac accaacagca 2881 cgcatgatgt ctactgggga tttattcttt agtgcggggg aatacacggc ttttaatacg 2941 attgagggcg tctcctaaca agttacatca ctcctgccct tcctcaccct catctccatc 3001 acctccttca tctccgtcat ctccgtcatc accctccgcg gcagcccctt ccaccatagg 3061 tggaaaccag ggaggcaaat ctactccatc gtcaaagctg cacacagtca ccctgatatt 3121 gcaggtagga gcgggctttg tcataacaag gtccttaatc gcatccttca aaacctcagc 3181 aaatatatga gtttgtaaaa agaccatgaa ataacagaca atggactccc ttagcgggcc 3241 aggttgtggg ccgggtccag gggccattcc aaaggggaga cgactcaatg gtgtaagacg 3301 acattgtgga atagcaaggg cagttcctcg ccttaggttg taaagggagg tcttactacc 3361 tccatatacg aacacaccgg cgacccaagt tccttcgtcg gtagtccttt ctacgtgact 3421 cctagccagg agagctctta aaccttctgc aatgttctca aatttcgggt tggaacctcc 3481 ttgaccacga tgctttccaa accaccctcc ttttttgcgc ctgcctccat caccctgacc 3541 ccggggtcca gtgcttgggc cttctcctgg gtcatctgcg gggccctgct ctatcgctcc 3601 cgggggcacg tcaggctcac catctgggcc accttcttgg tggtattcaa aataatcggc 3661 ttcccctaca gggtggaaaa atggccttct acctggaggg ggcctgcgcg gtggagaccc 3721 ggatgatgat gactgactac tgggactcct gggcctcttt tctccacgtc cacgacctct 3781 ccccctggct ctttcacgac ttccccccct ggctctttca cgtcctctac cccggcggcc 3841 tccactacct cctcgacccc ggcctccact acctcctcga ccccggcctc cactgcctcc 3901 tcgaccccgg cctccacctc ctgctcctgc ccctcctgct cctgcccctc ctcctgctcc 3961 tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg cccctcctgc 4021 ccctcctgct cctgcccctc ctgcccctcc tcctgctcct gcccctcctg cccctcctcc 4081 tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc 4141 tcctgcccct cctgctcctg cccctcctgc tcctgcccct cctgctcctg cccctcctgc 4201 tcctgcccct cctgcccctc ctgcccctcc tcctgctcct gcccctcctg ctcctgcccc 4261 tcctgcccct cctgcccctc ctgctcctgc ccctcctcct gctcctgccc ctcctgcccc 4321 tcctgcccct cctcctgctc ctgcccctcc tgcccctcct cctgctcctg cccctcctcc 4381 tgctcctgcc cctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc 4441 tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc 4501 tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gcccctcctg cccctcctcc 4561 tgctcctgcc cctcctcctg ctcctgcccc tcctgctcct gcccctcccg ctcctgctcc 4621 tgctcctgtt ccaccgtggg tccctttgca gccaatgcaa cttggacgtt tttggggtct 4681 ccggacacca tctctatgtc ttggccctga tcctgagccg cccggggctc ctggtcttcc 4741 gcctcctcgt cctcgtcctc ttccccgtcc tcgtccatgg ttatcacccc ctcttctttg 4801 aggtccactg ccgccggagc cttctggtcc agatgtgtct cccttctctc ctaggccatt 4861 tccaggtcct gtacctggcc cctcgtcaga catgattcac actaaaagag atcaatagac 4921 atctttatta gacgacgctc agtgaataca gggagtgcag actcctgccc cctccaacag 4981 cccccccacc ctcatcccct tcatggtcgc tgtcagacag atccaggtct gaaaattccc 5041 catcctccga accatcctcg tcctcatcac caattactcg cagcccggaa aactcccgct 5101 gaacatcctc aagatttgcg tcctgagcct caagccaggc ctcaaattcc tcgtccccct 5161 ttttgctgga cggtagggat ggggattctc gggacccctc ctcttcctct tcaaggtcac 5221 cagacagaga tgctactggg gcaacggaag aaaagctggg tgcggcctgt gaggatcagc 5281 ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg 5341 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt 5401 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 5461 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 5521 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 5581 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 5641 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 5701 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 5761 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 5821 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 5881 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 5941 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 6001 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 6061 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 6121 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 6181 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 6241 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 6301 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 6361 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 6421 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 6481 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 6541 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 6601 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 6661 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 6721 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 6781 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 6841 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 6901 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 6961 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 7021 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 7081 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 7141 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttgaa gctgtccctg 7201 atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc 7261 ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa 7321 gacgtagccc agcgcgtcgg ccccgagatg cgccgcgtgc ggctgctgga gatggcggac 7381 gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga 7441 ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccctgc ttcatccccg 7501 tggcccgttg ctcgcgtttg ctggcggtgt ccccggaaga aatatatttg catgtcttta 7561 gttctatgat gacacaaacc ccgcccagcg tcttgtcatt ggcgaattcg aacacgcaga 7621 tgcagtcggg gcggcgcggt ccgaggtcca cttcgcatat taaggtgacg cgtgtggcct 7681 cgaacaccga gcgaccctgc agcgacccgc ttaacagcgt caacagcgtg ccgcagatcc 7741 cggggggcaa tgagatatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct 7801 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg 7861 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga 7921 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc 7981 ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc 8041 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt 8101 cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc 8161 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc 8221 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc 8281 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt 8341 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat 8401 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg 8461 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga 8521 gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta 8581 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc 8641 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc 8701 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac 8761 tcgtccggat cgggagatgg gggaggctaa ctgaaacacg gaaggagaca ataccggaag 8821 gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt 8881 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc 8941 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt 9001 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc cactggcccc 9061 gtgggttagg gacggggtcc cccatgggga atggtttatg gttcgtgggg gttattattt 9121 tgggcgttgc gtggggtcag gtccacgact ggactgagca gacagaccca tggtttttgg 9181 atggcctggg catggaccgc atgtactggc gcgacacgaa caccgggcgt ctgtggctgc 9241 caaacacccc cgacccccaa aaaccaccgc gcggatttct ggcgtgccaa gctagtcgac 9301 caattctcat gtttgacagc ttatcatcgc agatccgggc aacgttgttg ccattgctgc 9361 aggcgcagaa ctggtaggta tggaagatct atacattgaa tcaatattgg caattagcca 9421 tattagtcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt 9481 atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttgac 9541 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 9601 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 9661 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 9721 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 9781 tgtatcatat gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 9841 attatgccca gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag 9901 tcatcgctat taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt 9961 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 10021 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg 10081 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga 10141 tctctagaag ctgggtacca gctgctagcg ccaccatgga gaccaccccc ctgaacagtc 10201 aaaagcaact ctctgcctgt gaggatggag aggactgtca ggagaatggc gttcttcaaa 10261 aggttgtacc cacccctgga gataaggtgg aaagcggcca gatttccaac ggctacagcg 10321 ccgtgccatc acctggagcc ggggatgata cacggcacag cattcccgca accaccacga 10381 cactggttgc agagcttcac cagggcgaga gggagacgtg gggcaaaaag gtagattttc 10441 tgctttccgt gatcggttac gcggtcgatc tggggaacgt gtggaggttc ccttacattt 10501 gttaccaaaa cggagggggt gccttcttgc tgccctacac aattatggcg atttttgggg 10561 gtatcccgct cttctacatg gaactggcct tgggtcagta ccaccggaac ggctgcatta 10621 gcatttggcg caagatatgc cccattttca agggaatcgg atacgcaatc tgtataatcg 10681 cgttctatat tgcatcatat tacaacacca tcatggcctg ggccctttat tacttgatca 10741 gcagtttcac cgaccaattg ccgtggacga gctgtaagaa ctcatggaat accgggaact 10801 gtaccaacta cttctctgag gacagctcag gaggtagcgg gggtgaacag aagctgatta 10861 gcgaggagga cttgggcagt ccaggcggaa gcggcaacat cacttggaca ctccatagta 10921 cgagccccgc agaggagttt tacaccaggc acgtactgca gattcatagg agcaagggtc 10981 tgcaagacct gggcggtatt agctggcagc tcgcattgtg cataatgttg atctttacgg 11041 tgatctattt ctctatctgg aagggagtaa agacatccgg caaggtggtg tgggtgacgg 11101 cgaccttccc ctacattata ctcagcgtgt tgttggtgag gggggcaacc ctgcctgggg 11161 cctggcgagg cgtgctgttc tatctcaagc caaactggca gaagcttctt gaaaccggag 11221 tgtggatcga cgcggccgcg caaatcttct tttccctcgg tcccggcttc ggggtccttc 11281 tggcattcgc aagctacaac aagttcaaca ataactgcta ccaggacgcc ctcgtgacaa 11341 gtgtagtgaa ctgcatgacc tctttcgtga gcgggtttgt gatcttcaca gtactcggat 11401 atatggctga gatgaggaac gaagatgtta gtgaggttgc caaagacgct ggcccgagcc 11461 tgttgttcat cacatacgcc gaggctatcg cgaacatgcc cgcatctact ttcttcgcca 11521 tcatcttttt cctgatgctg ataacccttg ggctggatag caccttcgcc ggactggagg 11581 gtgtcatcac tgccgttctc gacgagttcc cccatgtgtg ggccaagcga cgggaaaggt 11641 tcgtgctggc cgtcgtaatc acatgcttct tcggctcact ggtgaccctt acctttggcg 11701 gtgcgtacgt ggtcaagctg ctcgaagagt acgccactgg accggcggtt ctgaccgtgg 11761 cattgatcga ggccgtggcc gtaagctggt tttacggcat cacgcagttc tgtagggacg 11821 tgaaagagat gctgggcttt tctcccggtt ggttttggag gatttgttgg gtagcaatta 11881 gccccctgtt tctccttttc attatttgct cttttctgat gtctccaccg cagctccgac 11941 tgtttcagta caactacccc tactggtcaa ttgtcctggg ctattgtatc ggcacctcaa 12001 gtttcatctg catccccaca tacatcgcat acagactgat aattacgccc ggtactttta 12061 aggagcgaat cattaaaagc ataacccccg aaacccccac cgagatccct tgtggcgata 12121 taagactcaa tgcggtctaa tagctcgag //