LOCUS Exported 2703 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Cloning Module. ACCESSION . VERSION . KEYWORDS pUG-UBC3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2703) AUTHORS Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M TITLE UbiGate: a synthetic biology toolbox to analyse ubiquitination. JOURNAL New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 2017 Nov 30. PUBMED 29194629 REFERENCE 2 (bases 1 to 2703) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2703 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 99..118 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 352..369 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(939..957) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 1025..1129 /gene="bla" /label=AmpR promoter CDS 1351..2142 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 2313..198 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 61 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 121 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 181 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 241 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 301 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca 361 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg 421 attcattaat cactctgtgg tctcaaatga caactccagc aaaaaagaga ctgatgtggg 481 atttcaagag actgcagaaa gatcctcctg tgggaataag tggagctcca caagataaca 541 atatcatgca ttggaatgct ctcatctttg gacctgagga cactccatgg gatggaggta 601 ctttcaagtt gactcttcac ttcaccgagg attatccgaa caaacccccg attgttcggt 661 ttgtttcacg gatgtttcat ccaaacattt atgctgatgg aagcatttgc ttggatatct 721 tgcaaaacca gtggagcccc atctacgacg ttgctgcagt tctcacatca attcagtctt 781 tgctctgtga cccaaaccct gactcaccag cgaacgcaga ggctgcaagg ttgttcagtg 841 agaataaaag agaatataac aggaaagtaa tcgagatcgt ggagcagagc tatgtataag 901 ctttgagacc acgaagtggc tcttcagtgg acgaaagggc ctcgtgatac gcctattttt 961 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa 1021 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat 1081 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgcgctcacg 1141 caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat 1201 ggcttgttat gactgttttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa 1261 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag 1321 ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc cgaagtatcg 1381 actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc 1441 gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagcga tattgatttg 1501 ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt 1561 ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt 1621 gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga 1681 gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat 1741 ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg 1801 gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta 1861 acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg 1921 tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac 1981 tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct 2041 tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc 2101 cattacgtaa aaggcgagat caccaaggta gtcggcaaat aactgtcaga ccaagtttac 2161 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag 2221 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg 2281 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 2341 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 2401 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt 2461 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 2521 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 2581 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 2641 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 2701 gag //