LOCUS Exported 2736 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Cloning Module. ACCESSION . VERSION . KEYWORDS pUG-UBC15 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2736) AUTHORS Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M TITLE UbiGate: a synthetic biology toolbox to analyse ubiquitination. JOURNAL New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 2017 Nov 30. PUBMED 29194629 REFERENCE 2 (bases 1 to 2736) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2736 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 124..141 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(744..762) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 830..934 /gene="bla" /label=AmpR promoter CDS 1156..1947 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 2118..2706 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2607..2626 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 61 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag 121 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc 181 gcgcgttggc cgattcatta atcactctgt ggtctcaaat gaccagctct tccgctcctt 241 cacgcaaggc tttaagcaag atcgcgtgta ataggttgca gaaagagctt tctgagtggc 301 aattgaatcc ccccactgga tttcgtcaca aagtcactga taatcttcaa aaatggacaa 361 tagatgttac cggagctcca gggacacttt acgctaatga gacttatcag cttcaggttg 421 aatttcctga acattaccct atggaagcac cccaggtagt gtttgtttct ccggcacctt 481 cacatccaca tatatacagc aatggacata tttgtttaga tattctatat gactcatggt 541 caccagcaat gacagtgaat tcagtctgca tcagcattct ttcgatgcta tcaagttcac 601 ccgcaaagca acgccctgcg gataacgatc gttatgtgaa gaactgtaag aatgggaggt 661 cacctaagga gacgaggtgg tggttccatg acgacaaggt ttgagctttg agaccacgaa 721 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat 781 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc 841 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg 901 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac 961 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg 1021 tttttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg 1081 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa 1141 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag 1201 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc 1261 tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc 1321 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct 1381 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac 1441 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat 1501 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg 1561 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat 1621 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg 1681 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac 1741 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc 1801 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa 1861 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc 1921 gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag 1981 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 2041 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa 2101 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 2161 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 2221 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg 2281 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 2341 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 2401 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 2461 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 2521 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 2581 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 2641 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 2701 tggaaaaacg ccagcaacgc ggccttttta cggttc //