LOCUS Exported 2736 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Cloning Module. ACCESSION . VERSION . KEYWORDS pUG-UBC18 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2736) AUTHORS Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M TITLE UbiGate: a synthetic biology toolbox to analyse ubiquitination. JOURNAL New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 2017 Nov 30. PUBMED 29194629 REFERENCE 2 (bases 1 to 2736) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2736 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 38..55 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(658..676) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 744..848 /gene="bla" /label=AmpR promoter CDS 1070..1861 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 2032..2620 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2521..2540 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc 61 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact 121 ctgtggtctc aaatgacaag ctcttcatct ccttcacgca aggcgttaag caagatcgcg 181 tgtaataggt tacaaaagga gttatcagag tggcaagtaa atccaccgac tgggtttaag 241 cacagagtta ctgataatct tcaaaaatgg gtaattgagg taacaggagc acctggtacg 301 ctttacgcta atgaaactta taatcttcag gttgagtttc ctcaacatta tcctatggaa 361 gctcctcagg tgatttttgt tccaccagca ccgttacatc ctcatattta cagcaatgga 421 catatttgtt tagatattct gtatgactca tggtcaccag caatgacagt gagttcagtc 481 tgcatcagca tcctctcaat gctatcaagt tcccccgaaa agcaacgccc gactgataac 541 gatcgatatg tgaagaactg caagaacggg aggtcaccta aagagacaag gtggtggttc 601 catgacgaca aggtttaagc tttgagacca cgaagtggct cttcagtgga cgaaagggcc 661 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag 721 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 781 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 841 ggaagagtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg 901 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tttggggtac agtctatgcc 961 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag 1021 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc 1081 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct 1141 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc 1201 acacagcgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg 1261 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg 1321 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa 1381 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc 1441 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc 1501 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga 1561 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg 1621 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc 1681 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat 1741 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga 1801 tcagttggaa gaatttgtcc attacgtaaa aggcgagatc accaaggtag tcggcaaata 1861 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt 1921 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga 1981 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc 2041 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt 2101 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc 2161 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc 2221 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg 2281 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg 2341 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga 2401 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc 2461 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg 2521 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg 2581 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt 2641 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc 2701 tgattctgtg gataaccgta ttaccgcctt tgagtg //