LOCUS Exported 2724 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Cloning Module. ACCESSION . VERSION . KEYWORDS pUG-UBC21 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2724) AUTHORS Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M TITLE UbiGate: a synthetic biology toolbox to analyse ubiquitination. JOURNAL New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 2017 Nov 30. PUBMED 29194629 REFERENCE 2 (bases 1 to 2724) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2724 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 36..53 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(644..662) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 730..834 /gene="bla" /label=AmpR promoter CDS 1056..1847 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 2018..2606 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2507..2526 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg 61 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatcactct 121 gtggtctcaa atgcaggcat caagagcgcg actgtttaaa gaatacaaag aggtacagcg 181 agagaaagta gcagatcctg atattcaatt gatctgtgac gataccaaca tattcaaatg 241 gaccgctctt atcaaaggac cttcggagac tccttacgaa ggcggtgttt ttcagcttgc 301 tttttctgtt cctgaacctt atcctttgca acctcctcaa gttcgattct tgaccaagat 361 attccatcct aatgttcatt tcaagacagg agaaatatgt ctcgacattt tgaagaatgc 421 ttggagtcct gcttggacgc ttcagtctgt gtgtagagct atcatagcat tgatggctca 481 tcctgagccg gacagtcctc ttaactgcga ctcagggaat cttctaagat ctggtgatgt 541 gagagggttc aattcaatgg cacaaatgta tacacgcctc gccgctatgc ctaagaaagg 601 atgagctttg agaccacgaa gtggctcttc agtggacgaa agggcctcgt gatacgccta 661 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg 721 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 781 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgcgc 841 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt 901 ttcatggctt gttatgactg tttttttttg gggtacagtc tatgcctcgg gcatccaagc 961 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc 1021 agcagggcag tcgccctaaa acaaagttaa acatcatgag ggaagcggtg atcgccgaag 1081 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc 1141 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agcgatattg 1201 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg 1261 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca 1321 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat 1381 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca 1441 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag 1501 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa 1561 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta 1621 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg 1681 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac 1741 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat 1801 ttgtccatta cgtaaaaggc gagatcacca aggtagtcgg caaataactg tcagaccaag 1861 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 1921 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 1981 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 2041 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 2101 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 2161 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 2221 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 2281 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 2341 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 2401 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 2461 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 2521 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 2581 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 2641 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 2701 accgtattac cgcctttgag tgag //