LOCUS Exported 4974 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Cloning Module. ACCESSION . VERSION . KEYWORDS pUG-UBC24 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4974) AUTHORS Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M TITLE UbiGate: a synthetic biology toolbox to analyse ubiquitination. JOURNAL New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 2017 Nov 30. PUBMED 29194629 REFERENCE 2 (bases 1 to 4974) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4974 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 52..69 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(2910..2928) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 2996..3100 /gene="bla" /label=AmpR promoter CDS 3322..4113 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 4284..4872 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4773..4792 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca 61 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg 121 attcattaat cactctgtgg tctcaaatgg aaatgtccct tactgactct gattgggata 181 gctccagcga cagtggtagc agtgaacacg aagaagtcga gttttcttat ggtggacggg 241 cacagaacat tttctcaaac cttgaagaaa ccattggcaa aatcgatgag ttcctgtcgt 301 tcgagagggg atttatgtat ggtgacattg tgcggtccgc cactgaacca tcaggacaga 361 gtggcagggt tatcaacata gacatgtttg tcaatctcga aagtactcat gggaagatca 421 tgaaggaagt tgataccaag aggcttcaaa agttgcgttc tatttcactc tctgattatg 481 tgattaacgg accttgggtt ggaagggttg acaaaatagt tgagcgtgtc tctgtcaccc 541 ttgatgatgg gaccaactat gaggtccttg tagatggtca agataaactt gtggccattc 601 ccccaaattt actcgaggat tctcaatatt cgtattaccc agggcaaaga gttcaggtaa 661 agctggccca tgcccccaga tcaactacat ggttatgcgg gacctggaga ggaacccagg 721 ttatgggaac tgtttgcact gtagaagcag gacttgtcta cgtcgattgg gttgcctcca 781 tcgtaatgga gggtgatcgg aatttaactg cacctcaagc tttgcagaat cctgagagtt 841 taactttgtt accttgtgtt tctcatgcga gttggcagct tggtgactgg tgtatactcc 901 ctggctcttc ccactgtgat atagcagagc ggcaaactcc aaatgtggct gcctacaatc 961 tcaatgaatg ccataagaca ttccaaaaag ggtttaacag aaatatgcag aactcaggtt 1021 tggatgagct atttgttatc acaaagacaa agatgaaggt tgctgttatg tggcaagatg 1081 gtagctgcag tctgggagtt gattcccaac agctgcttcc tgttggtgct gttaatgctc 1141 atgatttttg gcccgaacag tttgttgtgg aaaaggaaac ctgcaacagc aaaaaatggg 1201 gagttgtgaa ggctgtcaat gctaaggaac aaactgtgaa ggtacaatgg acaatacagg 1261 ttgagaaaga agcaacaggt tgtgttgatg aggtgatgga agaaattgtc agtgcatatg 1321 aactgcttga gcaccctgat tttggattct gtttcagcga tgtggtggtc aagttacttc 1381 cagaaggaaa atttgatcca aatgcagaca caatcgtcgc tacagaggcg aaacacctac 1441 ttacagagag tgactacagt ggcgcatatt ttttgtcaag tattggtgtt gttacaggtt 1501 ttaaaaatgg ttccgtgaag gtgaaatggg ccaatggttc tactagcaag gttgcaccat 1561 gtgaaatttg gaaaatggaa aggtctgaat attccaactc tagcactgta agttcggaag 1621 gcagtgttca agatctaagt cagaagatta gtcaatcgga tgaagcatct tcaaaccatc 1681 aggaaacggg tctggtgaag ctctacagtg ttggtgaaag ttgcaacgag aacattccgg 1741 aatgtagttc atttttcctt ccaaaagctg ccattggatt tatcacaaac cttgcatcaa 1801 gcctttttgg ttatcagggt tccacttcgg ttataagttc acattcacgt tgcaatgatt 1861 ctgaagatca aagtgactct gaggtccttg ttcaagaaac agcagaatca tatgacaact 1921 ctgaaacaaa ttcaggtgaa gtggatatga ccaccacgat ggtcaacata cctatagaag 1981 gaaaaggaat taacaagaca ctggattcaa ctcttctaga gaacagcagg aaccaagtga 2041 gattcagaca gtttgatatg gttaatgact gctcggacca tcatttcctt tcttcggata 2101 aagggttggc tcagtcccag gtcacaaaga gttgggtgaa gaaagtccag caagaatgga 2161 gcaatttgga ggcaaatctt ccgaacacga tatatgtgcg tgtgtgtgaa gaaaggatgg 2221 accttctgcg tgcagccctg gttggtgccc ctggaacgcc atatcacgat gggcttttct 2281 ttttcgacat aatgcttcca cctcaatatc ctcatgagcc accaatggta cattatcatt 2341 caggtgggat gcgactgaat ccgaacctgt atgagtcagg aagagtttgc ttgagtctgc 2401 tgaatacatg gagtggctct ggcactgaag tatggaacgc agggagctcc tccatccttc 2461 aacttcttct ttcgtttcag gctctggttc tgaatgagaa gccttacttc aatgaagctg 2521 gctatgataa gcagttgggc cgagccgagg gagagaaaaa ctcagtgagt tacaatgaga 2581 atgcattcct cataacctgc aaatccatga tctcaatgct ccgtaagcct ccaaagcatt 2641 ttgagatgct tgtgaaggac cattttacgc accgggccca gcatgttctg gctgcgtgca 2701 aggcttatat ggaaggcgtc cctgtaggat catcagctaa cctgcagggg aactcaacca 2761 caaattccac cggtttcaag atcatgctct ccaaactcta cccaaaactt cttgaagcat 2821 tctcagagat tggagttgat tgcgtccaag agattggacc agaatcataa gctttgagac 2881 cacgaagtgg ctcttcagtg gacgaaaggg cctcgtgata cgcctatttt tataggttaa 2941 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg 3001 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata 3061 accctgataa atgcttcaat aatattgaaa aaggaagagt atgcgctcac gcaactggtc 3121 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta 3181 tgactgtttt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac 3241 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc 3301 cctaaaacaa agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta 3361 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg 3421 tacggctccg cagtggatgg cggcctgaag ccacacagcg atattgattt gctggttacg 3481 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact 3541 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac 3601 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag 3661 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc 3721 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc 3781 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg 3841 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt 3901 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg 3961 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga 4021 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccattacgta 4081 aaaggcgaga tcaccaaggt agtcggcaaa taactgtcag accaagttta ctcatatata 4141 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 4201 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 4261 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 4321 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 4381 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg 4441 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 4501 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 4561 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 4621 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 4681 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 4741 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 4801 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 4861 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 4921 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tatt //