LOCUS Exported 2829 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Cloning Module. ACCESSION . VERSION . KEYWORDS pUG-UBC27 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2829) AUTHORS Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M TITLE UbiGate: a synthetic biology toolbox to analyse ubiquitination. JOURNAL New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 2017 Nov 30. PUBMED 29194629 REFERENCE 2 (bases 1 to 2829) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2829 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 177..194 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(890..908) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 976..1080 /gene="bla" /label=AmpR promoter CDS 1302..2093 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 2264..23 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2753..2772 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 61 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 121 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg 181 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt 241 ggccgattca ttaatcactc tgtggtctca aatgatagat ttcagtcgaa tccagaaaga 301 gcttcaggac tgcgagagaa accaggattc ttcgggtatc cgggtctgcc ccaaatccga 361 taacctcacc cgactcaccg gaaccattcc tggtccaatc ggtactcctt acgaaggcgg 421 tacatttcag atcgatatca ctatgccaga tgggtatcca tttgagcctc caaagatgca 481 gttttcaact aaagtttggc atccgaatat tagtagccaa agcggtgcaa tatgcttgga 541 tatcttgaaa gaccagtgga gtccagctct tactctcaag acagctcttg tttcaattca 601 ggctttactc tctgcacctg agcccaaaga ccctcaagat gctgttgtag ctgaacagta 661 catgaagaac tatcaagtgt ttgtatcaac agctcgttac tggactgaaa ctttcgccaa 721 gaaatcttct ctagaggaaa aagtcaagag acttgtggag atgggttttg gagacgctca 781 agttaggagt gcaattgaat caagtggtgg tgatgagaat ctggctctgg aaaagctctg 841 ttctgcttag gctttgagac cacgaagtgg ctcttcagtg gacgaaaggg cctcgtgata 901 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact 961 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg 1021 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt 1081 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg 1141 gcggttttca tggcttgtta tgactgtttt tttttggggt acagtctatg cctcgggcat 1201 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg 1261 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgagggaa gcggtgatcg 1321 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga 1381 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagcg 1441 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga 1501 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag 1561 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac 1621 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga 1681 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag 1741 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa 1801 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag 1861 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg 1921 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag 1981 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg 2041 aagaatttgt ccattacgta aaaggcgaga tcaccaaggt agtcggcaaa taactgtcag 2101 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 2161 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 2221 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 2281 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 2341 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 2401 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 2461 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 2521 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 2581 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 2641 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 2701 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 2761 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 2821 gatgctcgt //