LOCUS Exported 2715 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Cloning Module. ACCESSION . VERSION . KEYWORDS pUG-UBC31 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2715) AUTHORS Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M TITLE UbiGate: a synthetic biology toolbox to analyse ubiquitination. JOURNAL New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 2017 Nov 30. PUBMED 29194629 REFERENCE 2 (bases 1 to 2715) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2715 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 43..60 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(642..660) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 728..832 /gene="bla" /label=AmpR promoter CDS 1054..1845 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 2016..2604 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2505..2524 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag 61 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 121 tcactctgtg gtctcaaatg tttaagaaaa tggataaaaa agcagcgcag agaattgcga 181 tggaatacag agctatgatc tcgaaagaat ctttgttcag tattggtcaa aactcgaaca 241 atatatacga atggactgca gtgatccggg gtccagatgg cactccctat gaaggtggca 301 tgtttaatct cagtattaag tttcctacgg attatccttt taaaccaccc aagtttacgt 361 ttaaaactcc gatttaccat ccaaatatca atgatgaagg atcgatttgc atgaacattc 421 ttaaagacaa atggactcct gctcttatgg ttgaaaaggt gcttctgtca atacttttac 481 tattggaaaa accaaaccca gatgatcctc ttgtacctga aattggacag ctcttcaaga 541 ataacagatt ccaatttgac cagagagctc gagaattcac tgctcgacat gctaataatt 601 aagctttgag accacgaagt ggctcttcag tggacgaaag ggcctcgtga tacgcctatt 661 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg 721 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct 781 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgcgctc 841 acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt 901 catggcttgt tatgactgtt tttttttggg gtacagtcta tgcctcgggc atccaagcag 961 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag 1021 cagggcagtc gccctaaaac aaagttaaac atcatgaggg aagcggtgat cgccgaagta 1081 tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg 1141 gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag cgatattgat 1201 ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac 1261 cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc 1321 attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt 1381 ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt 1441 gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg 1501 gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc 1561 ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg 1621 ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc 1681 gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag 1741 gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt 1801 gtccattacg taaaaggcga gatcaccaag gtagtcggca aataactgtc agaccaagtt 1861 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg 1921 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga 1981 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 2041 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 2101 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 2161 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 2221 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 2281 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 2341 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 2401 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 2461 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 2521 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 2581 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 2641 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac 2701 cgtattaccg ccttt //