LOCUS Exported 2712 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Cloning Module. ACCESSION . VERSION . KEYWORDS pUG-UBC36 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2712) AUTHORS Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M TITLE UbiGate: a synthetic biology toolbox to analyse ubiquitination. JOURNAL New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 2017 Nov 30. PUBMED 29194629 REFERENCE 2 (bases 1 to 2712) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2712 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(565..583) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 651..755 /gene="bla" /label=AmpR promoter CDS 977..1768 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 1939..2527 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2428..2447 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2681..2698 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatcac tctgtggtct 61 caaatggcca atagtaatct tccccgacga atcatcaagg aaactcaacg tctgcttagt 121 gaacctgctc ctgggataag tgcgtctcca tcagaggaaa atatgagata tttcaatgtt 181 atgattcttg gtcctactca atctccttat gaaggaggag ttttcaagtt ggagctcttt 241 ttgcctgaag aatatcctat ggcagctccc aaggttaggt ttctcaccaa gatttaccat 301 cctaacatcg acaagcttgg aagaatctgc cttgacatac tgaaagacaa gtggagtcct 361 gcactacaaa tacgaactgt gctcttgagt atccaagctc ttttaagtgc accaaaccca 421 gatgatcctc tgtctgagaa catagctaag cattggaaga gcaatgaggc agaagctgtg 481 gaaacagcta aggaatggac tcgtctttac gcaagtggtg cttaagcttt gagaccacga 541 agtggctctt cagtggacga aagggcctcg tgatacgcct atttttatag gttaatgtca 601 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc 661 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 721 gataaatgct tcaataatat tgaaaaagga agagtatgcg ctcacgcaac tggtccagaa 781 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact 841 gttttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt 901 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa 961 aacaaagtta aacatcatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga 1021 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg 1081 ctccgcagtg gatggcggcc tgaagccaca cagcgatatt gatttgctgg ttacggtgac 1141 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc 1201 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga 1261 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa 1321 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct 1381 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga 1441 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc 1501 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta 1561 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg 1621 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga 1681 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccatt acgtaaaagg 1741 cgagatcacc aaggtagtcg gcaaataact gtcagaccaa gtttactcat atatacttta 1801 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa 1861 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 1921 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 1981 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 2041 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc 2101 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 2161 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 2221 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 2281 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 2341 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 2401 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 2461 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 2521 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 2581 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga 2641 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga 2701 agcggaagag cg //