LOCUS Exported 2727 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Cloning Module. ACCESSION . VERSION . KEYWORDS pUG-135 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2727) AUTHORS Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M TITLE UbiGate: a synthetic biology toolbox to analyse ubiquitination. JOURNAL New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 2017 Nov 30. PUBMED 29194629 REFERENCE 2 (bases 1 to 2727) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2727 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 57..74 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(668..686) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 754..858 /gene="bla" /label=AmpR promoter CDS 1080..1871 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 2042..2630 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2531..2550 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg 61 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt 121 ggccgattca ttaatcactc tgtggtctca aatgagttcg gaggaagcca aggtcgttgt 181 tccgaggaac tttagattgt tggaagagct tgaaagaggt gagaaaggta ttggagatgg 241 tactgttagc tatggaatgg atgatgctga tgatatttat atgcaatctt ggactggcac 301 cattcttggt cctcctaata ctgcatatga agggaaaatc tttcagctga agcttttctg 361 tggtaaggag taccctgaaa gtccaccgac tgtgaggttc cagacccgga taaacatggc 421 ttgtgttaac ccagaaactg gagtggttga accgagtctc ttccctatgc tcactaactg 481 gcggcgagaa tatacaatgg aggacatact ggttaagctg aaaaaagaaa tgatgacttc 541 gcataaccgg aagttagctc aacccccgga aggtaacgag gaagctaggg cagatccaaa 601 aggaccagct aaatgttgtg tgatgtgagc tttgagacca cgaagtggct cttcagtgga 661 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct 721 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc 781 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa 841 tattgaaaaa ggaagagtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc 901 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tttggggtac 961 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt 1021 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca 1081 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg 1141 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg 1201 gcctgaagcc acacagcgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa 1261 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg 1321 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt 1381 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta 1441 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac 1501 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg 1561 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg 1621 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca 1681 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc 1741 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct 1801 cgcgcgcaga tcagttggaa gaatttgtcc attacgtaaa aggcgagatc accaaggtag 1861 tcggcaaata actgtcagac caagtttact catatatact ttagattgat ttaaaacttc 1921 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 1981 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 2041 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 2101 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 2161 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact 2221 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 2281 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 2341 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 2401 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 2461 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 2521 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 2581 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 2641 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg 2701 cgttatcccc tgattctgtg gataacc //