LOCUS       Exported                6394 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Destination vector derived from MSCV-IRES-Thy1.1. Please note that 
            this plasmid contains attB1 and attB2 sites for use in a BP 
            reaction.
ACCESSION   .
VERSION     .
KEYWORDS    MSCV-IRES-Thy1.1 DEST
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6394)
  AUTHORS   Wu Y, Borde M, Heissmeyer V, Feuerer M, Lapan AD, Stroud JC, Bates 
            DL, Guo L, Han A, Ziegler SF, Mathis D, Benoist C, Chen L, Rao A
  TITLE     FOXP3 controls regulatory T cell function through cooperation with 
            NFAT.
  JOURNAL   Cell. 2006 Jul 28. 126(2):375-87.
  PUBMED    16873067
REFERENCE   2  (bases 1 to 6394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6394
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    574..915
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             982..1398
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1328..1350
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1366..1382
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    1447..1471
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1564..1588)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1638..2190
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1782..1799)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2009..2028
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2238..2726
                     /codon_start=1
                     /product="mouse GPI-anchored cell surface glycoprotein of 
                     the immunoglobulin superfamily"
                     /label=Thy1.1
                     /note="This allele of Thy1 is also known as CD90.1."
                     /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK
                     DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF
                     CELRVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL"
     LTR             2782..3296
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    3458..3474
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3482..3512)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3527..3548
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3665..3682)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3836..4424)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3916..3935)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4595..5455)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5218..5237
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5456..5560)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5628..5646
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5684..5706)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5806..5825
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6019..6041
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6169..6188
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             6388..510
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 gaccccacct gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa
       61 atacataact gagaatagag aagttcagat caaggttagg aacagagaga cagcagaata
      121 tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga
      181 tggtccccag atgcggtccc gccctcagca gtttctagag aaccatcaga tgtttccagg
      241 gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc
      301 tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac
      361 tcggcgcgcc agtcctccga tagactgcgt cgcccgggta cccgtattcc caataaagcc
      421 tcttgctgtt tgcatccgaa tcgtggactc gctgatcctt gggagggtct cctcagattg
      481 attgactgcc cacctcgggg gtctttcatt tggaggttcc accgagattt ggagacccct
      541 gcccagggac caccgacccc cccgccggga ggtaagctgg ccagcggtcg tttcgtgtct
      601 gtctctgtct ttgtgcgtgt ttgtgccggc atctaatgtt tgcgcctgcg tctgtactag
      661 ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc
      721 ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga
      781 ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag
      841 aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc
      901 gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt
      961 ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt
     1021 cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt
     1081 tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc
     1141 acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat
     1201 ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct
     1261 ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg
     1321 tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc
     1381 actccttctc taggcgccgg aattagatct ctcgagatcg atgcggccgc gtttaaacgt
     1441 catcaaacaa gtttgtacaa aaaagcaggc ttcgaaggag atagaaccaa ttctctaagg
     1501 aaatacttaa ccatggtcga ctggatccgg taccgaattc gcggccgcac tcgagatatc
     1561 tagacccagc tttcttgtac aaagtggttc gatgacggta tcgataagct tgatatcgaa
     1621 ttccgccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     1681 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     1741 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     1801 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     1861 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     1921 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     1981 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     2041 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     2101 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     2161 ggttttcctt tgaaaaacac gatgataata tggccacaac catgggtctt ttctgcagtc
     2221 accgtcctcg aggcaccatg aacccagcca tcagcgtcgc tctcctgctc tcagtcttgc
     2281 aggtgtcccg agggcagaag gtgaccagcc tgacagcctg cctggtgaac caaaaccttc
     2341 gcctggactg ccgccatgag aataacacca aggataactc catccagcat gagttcagcc
     2401 tgacccgaga gaagaggaag cacgtgctct caggcaccct tgggataccc gagcacacgt
     2461 accgctcccg cgtcaccctc tccaaccagc cctatatcaa ggtccttacc ctagccaact
     2521 tcaccaccaa ggatgagggc gactactttt gtgagcttcg cgtctcgggc gcgaatccca
     2581 tgagctccaa taaaagtatc agtgtgtata gagacaagct ggtcaagtgt ggcggcataa
     2641 gcctgctggt tcagaacaca tcctggatgc tgctgctgct gctttccctc tccctcctcc
     2701 aagccctgga cttcatttct ctgtgacgcg tagaattcga taaaataaaa gattttattt
     2761 agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa
     2821 gtaacgccat tttgcaaggc atggaaaata cataactgag aatagagaag ttcagatcaa
     2881 ggttaggaac agagagacag cagaatatgg gccaaacagg atatctgtgg taagcagttc
     2941 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtcccgcc ctcagcagtt
     3001 tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt
     3061 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc
     3121 tcaataaaag agcccacaac ccctcactcg gcgcgccagt cctccgatag actgcgtcgc
     3181 ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg
     3241 ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatgggt
     3301 aacagtttct tgaagttgga gaacaacatt ctgagggtag gagtcgaata ttaagtaatc
     3361 ctgactcaat tagccactgt tttgaatcca catactccaa tactcctgaa atagttcatt
     3421 atggacagcg cagaaagagc tggggagaat tgtgaaattg ttatccgctc acaattccac
     3481 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     3541 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     3601 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     3661 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     3721 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     3781 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     3841 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     3901 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     3961 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     4021 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     4081 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     4141 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     4201 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     4261 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     4321 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     4381 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     4441 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     4501 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     4561 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     4621 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     4681 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     4741 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     4801 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     4861 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     4921 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     4981 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     5041 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     5101 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     5161 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     5221 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     5281 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     5341 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     5401 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     5461 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     5521 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     5581 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     5641 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     5701 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     5761 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     5821 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     5881 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     5941 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     6001 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggcgcaa ggaatggtgc
     6061 atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc
     6121 gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc
     6181 gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc
     6241 gtagaggcga ttagtccaat ttgttaaaga caggatatca gtggtccagg ctctagtttt
     6301 gactcaacaa tatcaccagc tgaagcctat agagtacgag ccatagataa aataaaagat
     6361 tttatttagt ctccagaaaa aggggggaat gaaa
//
