LOCUS       Exported               14168 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Donor construct for introduction of hNIL factors and SBP-LNGFR for 
            streptavidin bead enrichment.
ACCESSION   .
VERSION     .
KEYWORDS    CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14168)
  AUTHORS   Fernandopulle MS, Prestil R, Grunseich C, Wang C, Gan L, Ward ME
  TITLE     Transcription Factor-Mediated Differentiation of Human iPSCs into 
            Neurons.
  JOURNAL   Curr Protoc Cell Biol. 2018 Jun;79(1):e51. doi: 10.1002/cpcb.51. 
            Epub 2018 May 18.
  PUBMED    29924488
REFERENCE   2  (bases 1 to 14168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14168
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(171..1031)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     protein_bind    1889..1922
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    1923..1948
                     /label=SA
                     /note="splice acceptor site"
     CDS             1972..2025
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2035..2634
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2035..2054)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2531..2551
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    2655..2879
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        2915..3126
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3139..3407
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      3445..3454
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3532..3645
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             4429..4482
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      4489..4498
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     protein_bind    5212..5245
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     complement(5261..5278)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5267..5491
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        5497..5876
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5878..6155
                     /label=chicken beta-actin promoter
     intron          6157..7174
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     7098..7120
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     7182..7201
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             7233..7979
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(8070..8089)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8135..8190
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8189..8208)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     8500..8517
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8634..8655
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8670..8700
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8708..8724
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8713..8735
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8732..8748
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8732..8748
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    8797..8918
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8808..8827)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8862..8881
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(10150..10203)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      10475..10484
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             complement(11275..11331)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(12251..12275)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    12320..12338
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12356..12374
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12392..12410
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12428..12446
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12464..12482
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12500..12518
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12536..12554
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     rep_origin      complement(13580..14168)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13660..13679)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatag caggagaatc atgccaatgg gccaatatac
     1081 attctgaccc acagtttcat aataaaataa aatggttgtg gttgtaagcc actatgtttc
     1141 agagtggttt gttacacagc aataaataac taatatagta ggcataccat caagtccaaa
     1201 gtaggtagag aagaatgtaa atagcagagc aaaacagcat gactggtggc tgggaggctt
     1261 aaaactggga caggatcaga gtcatgaaag aagtcaaaga aatggttcag aagtaaggct
     1321 gagactgact tacaaaagct gaaagtccct ttaagttggt gtttggtgca ttggcagggg
     1381 caggtatggt gacttaaaag agccatgctc aacaagatca agcacaacac aatcacgggt
     1441 caccccagca gaccttagcg agtctagcca tttctttggt ggtggtcaca gtcatgcttc
     1501 agcccagttt ccacttggac aaatggtaca tattttcaat gagatgaaaa ttaagataca
     1561 atccatgtgc tcagagagtg atcacagctc tgactaaaca ctgtgcccca aagtgttgag
     1621 gaattgggaa aacctagctg agttagtggt ctcttttctg ttacaataaa gctcataatg
     1681 aaaattagcc ttctttgttc ttccccaagt ctttctttct agacgaaact actttcaact
     1741 gttttaactt ccttactgtt aacttccata tttctagata gtatggtcag actgttattt
     1801 cttgactttt aaatgtaaga tattatctac tgacttcctt ctatgtaaga tgaggattga
     1861 gctctcttac ccttctccca gcggccgcat aacttcgtat agcatacatt atacgaagtt
     1921 atctgacctc ttctcttcct cccacagggc ctcgagagat ctggcagcgg agagggcaga
     1981 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctaggct cgagatgacc
     2041 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     2101 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     2161 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     2221 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     2281 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     2341 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     2401 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     2461 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     2521 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     2581 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgatctaga
     2641 gggcccgttt aaacctgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     2701 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     2761 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     2821 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatgga
     2881 tgcatgatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     2941 cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg
     3001 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     3061 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     3121 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga
     3181 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga
     3241 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg
     3301 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct
     3361 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg
     3421 gcgcctacgc tagcgctacc ggtcgccacc atgggctggt catgtatcat tctgtttctg
     3481 gtcgcaaccg caactggagt gcattcacag gtgcagctcg aggggtcagg gatggacgaa
     3541 aagaccacag gatggcgagg aggacacgtg gtcgagggac tggcaggaga gctggaacag
     3601 ctgcgggcta gactggaaca ccatcctcag ggacagcgag agccaggaag tggagcgatc
     3661 gcgaaggaag cctgccccac agggctgtac actcattctg gagaatgctg taaagcttgt
     3721 aacctgggag agggagtggc acagccatgc ggagccaatc agactgtgtg cgagccttgt
     3781 ctggactccg tcacattctc tgatgtggtc agtgccacag aaccttgcaa gccatgtact
     3841 gagtgcgtgg gcctgcagtc tatgagtgct ccttgtgtgg aggctgacga tgcagtctgc
     3901 cggtgtgcat acggatacta tcaggacgag actaccggca gatgtgaagc ttgcagggtg
     3961 tgtgaggcag gctcagggct ggtctttagc tgccaggata aacagaacac cgtgtgcgag
     4021 gaatgtcctg acgggacata tagcgatgag gccaatcacg tggacccctg cctgccttgt
     4081 actgtgtgcg aggataccga aaggcagctg cgcgaatgta ccagatgggc agacgccgag
     4141 tgcgaggaaa tcccagggcg atggattact cggtccaccc cccctgaagg atcagacagc
     4201 accgcaccat ctacacagga gccagaagca ccaccagagc aggatctgat cgcctccacc
     4261 gtggctggcg tggtcacaac tgtcatgggg agctcccagc cagtggtcac ccggggcacc
     4321 acagataacc tgattcccgt gtattgctcc atcctggcag ccgtcgtcgt cggactggtg
     4381 gcatacatcg ccttcaagcg gcaattgatg cacagcagag gctctggcga gggcagagga
     4441 agtcttctaa catgcggtga cgtggaggag aatcccggcc cttcaaacgc caccatggtg
     4501 tccaagggcg aagagaacaa catggccatc atcaaagaat tcatgcggtt caaggtgcac
     4561 atggaaggca gcgtgaacgg ccacgagttc gagattgaag gcgaaggcga gggcagaccc
     4621 tacgaggctt ttcagaccgc caagctgaaa gtgaccaaag gcggccctct gccttttgcc
     4681 tgggacattc tgagccctca gtttatgtac ggcagcaagg tgtacatcaa gcaccccgcc
     4741 gacattcccg actacttcaa gctgagcttc cccgagggct tcagatggga gagagtgatg
     4801 aacttcgagg acggcggcat catccacgtg aaccaggata gctctctgca ggacggcgtg
     4861 ttcatctaca aagtgaagct gcggggcacc aactttccct ctgatggccc cgtgatgcag
     4921 aaaaagacca tgggctggga agccagcgag gaaagaatgt accctgagga tggcgccctg
     4981 aagtccgaga tcaagaagcg gctgaagctg aaggatggcg gccactatgc cgccgaagtg
     5041 aaaaccacct acaaggccaa gaaacccgtg cagctgcctg gcgcctacat cgtggatatc
     5101 aagctggaca tcgtgtccca caacgaggac tacaccatcg tggaacagta cgagagagcc
     5161 gaaggcagac acagcacagg cggaatggac gagctgtaca agtaaggtac cataacttcg
     5221 tatagcatac attatacgaa gttataaacc cgctgatcag cctcgactgt gccttctagt
     5281 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     5341 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     5401 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     5461 aggcatgctg gggatgcggt gggctctatg ggtctcgaca ttgattattg actagttatt
     5521 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     5581 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     5641 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     5701 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     5761 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     5821 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg
     5881 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt
     5941 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggggc
     6001 gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg
     6061 cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc
     6121 ggcggcccta taaaaagcga agcgcgcggc gggcggggag tcgctgcgac gctgccttcg
     6181 ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt
     6241 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt
     6301 ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg
     6361 ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
     6421 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
     6481 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
     6541 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
     6601 tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca
     6661 cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg
     6721 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg
     6781 ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg
     6841 cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat
     6901 ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa
     6961 gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg
     7021 ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct gccttcgggg
     7081 gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc
     7141 taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt
     7201 gctgtctcat cattttggca aagaatgtcg acatgagccg gctggacaag agcaaagtga
     7261 tcaacagcgc cctggaactg ctgaacggcg tgggcatcga gggcctgacc acccggaagc
     7321 tggcccagaa actgggcgtg gaacagccca ccctgtactg gcacgtgaag aacaagcggg
     7381 ccctgctgga cgccctgccc atcgagatgc tggaccggca ccacacccac agctgccctc
     7441 tggaaggcga gagctggcag gacttcctgc ggaacaacgc caagagctac agatgcgccc
     7501 tgctgagcca ccgggacggc gccaaagtgc acctgggcac cagacccacc gagaagcagt
     7561 acgagacact ggaaaaccag ctggccttcc tgtgccagca gggcttcagc ctggaaaacg
     7621 ccctgtacgc cctgagcgcc gtgggccact tcaccctggg ctgcgtgctg gaagaacagg
     7681 aacaccaggt cgccaaagag gaaagagaga cacccaccac cgacagcatg ccccccctgc
     7741 tgaagcaggc catcgagctg ttcgacagac agggcgccga gcccgccttc ctgttcggcc
     7801 tggaactgat catctgcggc ctcgagaagc agctgaagtg cgagagcggc ggacccaccg
     7861 acgccctgga cgacttcgac ctggacatgc tgcccgccga tgccctggat gattttgatc
     7921 tggatatgct ccctgctgac gctctcgatg acttcgatct cgatatgctg ccaggctaaa
     7981 acgcgtgaat tcactcctca ggtgcaggct gcctatcaga aggtggtgac tggtgtggcc
     8041 aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac
     8101 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca
     8161 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat
     8221 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct
     8281 gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc
     8341 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt
     8401 gtgttatttt tttctttaac atccctaaaa ttttccttac atgtgcggcc gtcctttgag
     8461 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     8521 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     8581 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     8641 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     8701 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     8761 aagctctagc tagaggtcga cggtatacag acatgataag atacattgat gagtttggac
     8821 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     8881 ctttatttgt aaccattata agctgcaata aacaagttgg ggtgggcgat cgattcagaa
     8941 ctgggcgtga tccacttcgt ccagccagga ggcagggctg gcaggaaaat cggggtagcc
     9001 gccagagctg cctgtgctca gatcgctgct agggccattg ccggccagca ctctcattgg
     9061 aggaggtccg cctggagcgc cagatggcac caggcccagg cttgtatcgg ggtacaccag
     9121 gctggacagc agaggctgag gtcctggcag agactgaggg gcagctggag aaggtggcac
     9181 gccgtaaggg ctgccgggcc tcagttctct atactgctcg ggtccggcca gtccgccgtg
     9241 ttccagagag aagttgccca gggcgccaga aggtctgccc agagcctggg ttggctctcc
     9301 caggctgcca tacaggccat tggctgggcc catttcggcc aggctaggct catctgggaa
     9361 ggacacctcg gcgtcgctgt cctggccttc ctgcacgctg tccttgtcgc tcttgctgcc
     9421 gcctctggac cgcttcatgt tccggaagta ctggccccat ctctgcctgc cggcgtcctt
     9481 cttcagccgc ttctctttgg cccgtctgtt ctggaaccac acttgcacca ctctcatgtc
     9541 caggcctgtc tcgctgctca gctgttcccg cacatgtctg gcgggcttgg ggctggtgtt
     9601 gtaggcggac ttcagggttt ccagctgctt ggcggtgatg gtggttctgg gtcttttggc
     9661 ggtggcctcg gcctctctct gcttggctgt ctcgtagtcg gctttacaca cgagccggct
     9721 atcttccatc aggtagaact cgtcgccggt ggccagctgt ctcttgcaca cgacgcaggc
     9781 gaagcagtgc agatggtaca cgaagtcctg ggctctccgc acgacctgtg taggaggaat
     9841 tcccagctgg caggcggcgc acttggtgcc gaatctcttg aagaagtcgt ccttgcagta
     9901 cacgctctcg cctctgctaa agcatctctc ggccaggggg gtgtggcagt cgctgcactt
     9961 caggcacttg ctgtgccagt gtctgtccag ggccttcagg ataaaccggt ccaggatgtg
    10021 ctggtcgcag ccggcgcaca gggggatttc tcttctcagg tcggcccttc tagccagcag
    10081 ggccagcagc agatctccgc cggcagattc tctggcaggg cccagttcgc ctctggcttc
    10141 catggtaccg gggccagggt tctcttccac gtcgccacat gtcagcaggc tgcctctgcc
    10201 ctcgccagag cctctgctgt gcatcaatgc ctcaatagga ctggctacca tgctgttagg
    10261 tgtatctgga agttgagagg acattgatgc tacttcactg ccagtggaat tagagcccgg
    10321 tcctccttct gaaaaattga ccagttgctg aaaagcaggc tgatctatgt cactctgcaa
    10381 ggcgaagtcg ctcagtactt tccaaggtgg ctggtaactt tgtacttcca ctgggttagc
    10441 ctgtaagcca ccgtcgtgtc tctctggact ggcagccacc atgggagttc ctgtcatccc
    10501 ctggatatta gttttgtcat tgggctgctg ctgctggagt tgcttcatca tgatgcttcg
    10561 cttcttgtcc ttgcaccgct tgttttgaaa ccagacccgg atcacacggg gactgaggcc
    10621 cgtcatctct accagttgct ccttcatgag cgcatctggc cgcgggtttg cggcgtagca
    10681 ggtccgcaag gtgtgcagct gcttctcgtt cagcacagtc cgcacgcggg tggtcttctc
    10741 cggctgcttg tggacgtggg gccgcagggc cggctgcctg gcggagatgg gctccgctgc
    10801 catttgcagt ggccgcgctg gatgcagggg actgagcggg tcgccagcgc ctagactggc
    10861 cctctccacc acatcgtggt ctgctcggca gaagagaccg tcctcccgaa gcgcaaattc
    10921 gtccccaggg atgagctggc ggctgcaggc cacacagcgg aaacactcga tgtgatacac
    10981 cttggagcgg gcacgcatca cgaagtcgtt cttgctgaag ccgatgctgc acttggcgca
    11041 tttgatcccg tacaacctga tataatctct tttacagtag gttttcccat ccctaacaaa
    11101 gcatgtacag ctctcgtcca aatactgatt acactccgca catttcaaac atgccgcatg
    11161 ccattccaaa tccggagaaa ccctcagaat atactgatcg tgaatctgat tgccgcaacc
    11221 aacacatagg gaaatcagac gttttttttt tggtggatct cccatgtctc ccataggtcc
    11281 agggttctcc tccacgtctc cagcctgctt cagcaggctg aagttagtag ctccggatcc
    11341 caagatacaa tccctggcta tggggaggtg aggtgcatag cggtgcttgt cgggaggtgg
    11401 gggctgccaa tagtccatgt ctgacccggc cgggctggcg ggcgataaag tgcagctgta
    11461 gggggaggtg gaattggagg acacggagga ggacggcgcg gggctgttgg tgcaactcca
    11521 cgtggaggcg ggcgaggggc tgtctccgct gctgctcagg gcggcgctgg cgcctcccgg
    11581 gctcagcaac actgcctcgg agaagagcgc ccccggcagg cccccgccgc cgcccccgca
    11641 gtgatccgcc aggcgcaggg tctcggtgag tgcccagatg tagttgtggg cgaagcgcag
    11701 ggtctcgatc ttggtgagct tggcgtcctc ggggaacgtg gggagcacct cgcgcagcgc
    11761 gtccagtgcc gcgttgaggt tgtgcatgcg gtttcgctcg cggttgttgg ccttcagtct
    11821 acgggtcttc ttgatgcgct gcaccgtctc ggccgtcttg gcgcctcggg agacggcccg
    11881 cgcccgggaa gggcgccgtt tgcaatcgtg taccagaccc agcagccgtg cgggccggca
    11941 gccctccgca cccgcagcca cgccgccccg cgccccctgc ccggcctcag ccccgcgctg
    12001 ccgacgcgcc ccgcctgacg cgcccggctc ctcctcctct tcttcgtcgg cgctggatga
    12061 cagcggggtc agggccgcca aggcggggga ggccgatccg agcagcacta acacgtcctc
    12121 ttcctccttc aactccaagg tctcggattt gacgaacatg gtggcggctt aattaacctt
    12181 aagtttacga gggtaggaag tggtacggaa agttggtata agacaaaagt gttgtggaat
    12241 tgctccaggc gatctgacgg ttcactaaac gagctctgct tttataggcg cccaccgtac
    12301 acgcctaaag cttatacgtt ctctatcact gatagggagt aaactggata tacgttctct
    12361 atcactgata gggagtaaac tgtagatacg ttctctatca ctgataggga gtaaactggt
    12421 catacgttct ctatcactga tagggagtaa actccttata cgttctctat cactgatagg
    12481 gagtaaagtc tgcatacgtt ctctatcact gatagggagt aaactcttca tacgttctct
    12541 atcactgata gggagtaaac cactgtgggg tggagatatc tcctcatcct tccaacataa
    12601 atatattttg ggattatatc aacattcaat gttacttaaa gtgaccttgt aaatattttc
    12661 acaactgagc catgtttgat ttgtatactt atgtttactt tactgttttt cctgaagtta
    12721 ataattgcct tgaatttatt tatttcttta aaaatgtttc attactcagg actgtagttt
    12781 acattacgat tctttgtgtt atacagttga tgggtttctt ttctttctta atttctttaa
    12841 aaaatagaga tggggtctta ctatattacc caggctggtc ttgaagtcct gggctcaagt
    12901 gatcttcctg tctcagccta ccaagtagct gagactatag gtgcaaaaaa gccactatac
    12961 ctggctagtt tacaggtttt aacaaatgca ttatgccacg tatccattat tacaggatca
    13021 cacaagatat tttcattacc ctgaaagcat ccctgtgttc caccaattca tcctgcctcc
    13081 atgagccgct ggcaaccact gatctctata gttttgcctt ttctaaaatg tcatataatt
    13141 ggaatcatac agtctgtagc attttcagac tagcttttaa aatttggcaa tatgcattta
    13201 aggttcctcc ttaaatgtga agggcatagc caatgtggct tgatagctca tttcttttta
    13261 ttggtgaata tttcattgtc tggatgttcc acagtttgtt tatccattca cctattcaat
    13321 ttgctttttt tctgtgtatc tatcactaat tcaatactgg actctccaac agagccgtcc
    13381 cgcttcccta aggccctgct ctgggcttct gggtttgagt ccttggcaag cccaggagag
    13441 gcgctcaggc ttccctgtcc cccttcctcg tccaccatct catgcccctg gctctcctgc
    13501 cccttcccta caggggttcc tggctctgct ctaagggcga attcgccagg aaccgtaaaa
    13561 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    13621 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    13681 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    13741 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    13801 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    13861 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    13921 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    13981 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
    14041 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    14101 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    14161 gatctcaa
//
