LOCUS Exported 3912 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Cloning Module. ACCESSION . VERSION . KEYWORDS pUG-234 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3912) AUTHORS Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M TITLE UbiGate: a synthetic biology toolbox to analyse ubiquitination. JOURNAL New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 2017 Nov 30. PUBMED 29194629 REFERENCE 2 (bases 1 to 3912) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3912 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 39..56 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(1835..1853) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 1921..2025 /gene="bla" /label=AmpR promoter CDS 2247..3038 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 3209..3797 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3698..3717 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 61 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatcac 121 tctgtggtct caaatggcgg gtatcaatag agcgggacca tcgggcgctt attttgttgg 181 ccacacagac cccgagccag tatcggggca agcacacgga tccggcagcg gcgccagctc 241 ctcgaacagt ccgcaggttc agccgcgacc ctcgaatact cccccgtcga acgcgcccgc 301 accgccgcca accggacgtg agaggctttc acgatccacg gcgctgtcgc gccaaaccag 361 ggagtggctg gagcagggta tgcctacagc ggaggatgcc agcgtgcgtc gtaggccaca 421 ggtgactgcc gatgccgcaa cgccgcgtgc agaggcaaga cgcacgccgg aggcaactgc 481 cgatgccagc gcaccgcgta gaggggcggt tgcacacgcc aacagtatcg ttcagcaatt 541 ggtcagtgag ggcgctgata tttcgcatac tcgtaacatg ctccgcaatg caatgaatgg 601 cgacgcagtc gctttttctc gagtagaaca gaacatattt cgccagcatt tcccgaacat 661 gcccatgcat ggaatcagcc gagattcgga actcgctatc gagctccgtg gagcgcttcg 721 tcgagcggtt caccaacagg cggcgtcagc gccagtgagg tcgcccacgc caacaccggc 781 cagccctgcg gcatcatcat cgggcagcag tcagcgttct ttatttggac ggtttgcccg 841 tttgatggcg ccaaaccagg gacggtcgtc gaacactgcc gcctctcaga cgccggtcga 901 caggagcccg ccacgcgtca accaaagacc catacgcgtc gacagggctg cgatgcgtaa 961 tcgtggcaat gacgaggcgg acgccgcgct gcgggggtta gtacaacagg gggtcaattt 1021 agagcacctg cgcacggccc ttgaaagaca tgtaatgcag cgcctcccta tccccctcga 1081 tataggcagc gcgttgcaga atgtgggaat taacccaagt atcgacttgg gggaaagcct 1141 tgtgcaacat cccctgctga atttgaatgt agcgttgaat cgcatgctgg ggctgcgtcc 1201 cagcgctgaa agagcgcctc gtccagccgt ccccgtggct cccgcgaccg cctccaggcg 1261 accggatggt acgcgtgcaa cacgattgcg ggtgatgccg gagcgggagg attacgaaaa 1321 taatgtggcc tatggagtgc gcttgcttaa cctgaacccg ggggtggggg taaggcaggc 1381 tgttgcggcc tttgtaaccg accgggctga gcggccagca gtggtggcta atatccgggc 1441 agccctggac cctatcgcgt cacaattcag tcagctgcgc acaatttcga aggccgatgc 1501 tgaatctgaa gagctgggtt ttaaggatgc ggcagatcat cacacggatg acgtgacgca 1561 ctgtcttgcg ggcggagaat tgtcgctgag taatccggat cagcaggtga tcggtttggc 1621 gggtaatccg acggacacgt cgcagcctta cagccaagag ggaaataagg acctggcgtt 1681 catggatatg aaaaaacttg cccaattcct cgcaggcaag cctgagcatc cgatgaccag 1741 agaaacgctt aacgccgaaa atatcgccaa gtatgctttt agaatagtcc cctgagcttt 1801 gagaccacga agtggctctt cagtggacga aagggcctcg tgatacgcct atttttatag 1861 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg 1921 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga 1981 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgcg ctcacgcaac 2041 tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct 2101 tgttatgact gttttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc 2161 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca 2221 gtcgccctaa aacaaagtta aacatcatga gggaagcggt gatcgccgaa gtatcgactc 2281 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac 2341 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagcgatatt gatttgctgg 2401 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg 2461 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg 2521 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat 2581 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg 2641 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg 2701 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc 2761 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc 2821 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg 2881 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc 2941 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccatt 3001 acgtaaaagg cgagatcacc aaggtagtcg gcaaataact gtcagaccaa gtttactcat 3061 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc 3121 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 3181 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 3241 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 3301 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc 3361 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 3421 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 3481 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 3541 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 3601 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 3661 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 3721 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 3781 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 3841 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta 3901 ccgcctttga gt //