LOCUS       Exported                3054 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    pUG-131
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     36..53
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(974..992)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1060..1164
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1386..2177
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2348..2936
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2837..2856
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
       61 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatcactct
      121 gtggtctcaa atggaagctt cacccaatga tcgacttcat tttggcaaaa tgggtttcgg
      181 gtgtaagcat tacaagagga gatgccaaat cagagctcca tgttgcaacg aaatcttcga
      241 ttgtcgccat tgtcacaacg agagcactag cacattgcgc aatatctacg accgtcacga
      301 tcttgttcgt caagacgtta aacaagtgat ttgttctgtt tgcgatacag agcagccggc
      361 agctcaagtt tgttcgaatt gtggtgtcaa catgggagaa tatttttgca gcatctgcat
      421 attctatgat gatgatactg aaaaacaaca gtttcattgc gatgactgtg gaatttgcag
      481 agttggtggg cgtgagaatt tcttccattg caagaagtgt ggatcttgtt atgcggttgg
      541 tctgcgcaac aaccatcgct gcgttgagaa ttcaatgcgt catcactgtc ccatttgtta
      601 cgagtacctt tttgactctc taaaggacac aaatgtgatg aaatgcgggc acacaatgca
      661 cgtagaatgc tacaacgaga tgatcaaacg tgacaagttt tgttgtccaa tttgctcgag
      721 gtcagtgatt gatatgtcta aaacatggca gagactcgat gaagagatcg aagccactgc
      781 tatgccttca gattaccgtg acaagaaggt ttggatactt tgcaacgatt gtaacgacac
      841 aacagaagtg cacttccaca taatcggaca gaaatgtgga cattgcagat catacaacac
      901 acgagcgatt gcgcctcctg ttcttcctca atgagctttg agaccacgaa gtggctcttc
      961 agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     1021 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     1081 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     1141 caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac cttgaccgaa
     1201 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttttg
     1261 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     1321 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     1381 acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     1441 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     1501 atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc gtaaggcttg
     1561 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     1621 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     1681 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     1741 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     1801 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     1861 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     1921 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     1981 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     2041 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     2101 tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc gagatcacca
     2161 aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag attgatttaa
     2221 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     2281 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     2341 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     2401 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     2461 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     2521 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     2581 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     2641 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     2701 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     2761 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     2821 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     2881 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2941 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     3001 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgag
//
