LOCUS       Exported               13151 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to early 
            endosome associated 1.
ACCESSION   .
VERSION     .
KEYWORDS    actc1b-mKate2-EEA1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13151)
  TITLE     Parton Lab Plasmids for in vivo cell biology in the zebrafish
REFERENCE   2  (bases 1 to 13151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13151
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1061..1077
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1097..1117
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    5092..5116
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      5117..5126
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             5123..5818
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(5820..5844)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10048..10068)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(10076..10092)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    10108..10242
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10132..10151)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10186..10205
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(10914..10930)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10914..10930)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(10967..10987)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10967..10985)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11006..11022)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11019..11041)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11030..11046
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11054..11084)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11099..11120
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11237..11254)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11408..11996)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11488..11507)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12167..13027)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12790..12809
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13028..13132)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 aggctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     1081 gattacgcca agctatcaac tttgtataga aaagttggca cgcgcagatt ccgtgtgggc
     1141 ctggtaatat cttaccattt taagctattt taagattagg gaaaaaaatt aaataaaact
     1201 actttaaaat tactttcctt aaaaagcaac atatttattt agtttttgtt taactggctc
     1261 aagattgtaa tattttactt ttacatgttt tttttaagta cctaaatgtg ttcaaaagta
     1321 aagtatgtta ttgttttttt ttttttaatg ttagctaagc tttaaattgt aatatattac
     1381 ttttaaaagt gactttttaa aaatgtaaat aaaaaagtaa ctataaatgt gtaatgcatg
     1441 ttatttctgt gggtttttta attttcatct caactgggct tacaatttta acataataaa
     1501 aatcaaatca aatcaaatca ggcccttatt tgtcacatac actcttgtat actgaaataa
     1561 actaaatgtt gagaaaaatt taaatacacc aaaataacgc ataccttttt tgtaattacc
     1621 taagtaaaac atttagttcc ttttttaagt gactcaagat tgtaatctat taattgtaaa
     1681 agataaaact agtgatcaat ctgacgtgct tctatgcagc ctgtgagatt aattgtgatg
     1741 tttaattatt aaacaattta aatggatcaa acatttcaca tgtgcttatt gtgtccaaca
     1801 aaaccagcag aagcacttaa aaatattgca tgacaacatg agtttttaat gtaaattata
     1861 tatacttcct aaacgaagtt aatcatattt tctaaaataa taatgtcatt gaaatattta
     1921 aataaatgat aanctttagt tgattcaaca taaaattgaa agcaataagg actttttttt
     1981 ttttacagcg gtgaagcaaa agcntgnaaa taatcatata aatataataa ataaataaat
     2041 aaaataatgg ttcttggtgc aaatatgaac actttaaata aatatagaat aaatatgatc
     2101 aataagtcat aacgacgtgt tttatgttga ttataattta aacatgtttc taacgcaagt
     2161 tttaaaaact gttttaaagt attattttaa gttataagat caatttgact caacattttg
     2221 tagtgaaaat atgttgtgta aaattaatat aatatacact ataaaaatgc aaggttccac
     2281 acaattcatt catgttgtcc caacacaaat cgattaagtt aacaaatata tgtggattga
     2341 acataaaaaa ttaagttgtc ccaatgaaat ctcaagaatt gtgttgtttc agctcatttt
     2401 aaataagtag ttttaacaag taaaaaataa catatttttg agtgtgtata tatatatatt
     2461 tatatattac aaaatataat aatataaaat gtaaaagata taaaccatgc tacatatatt
     2521 taagagctat tttgcacatt acttttttgc aagccatttg agtgtttttt tacaaatata
     2581 tattagctaa atatacaata tcttaaatat acaattaatc agacatgcaa aaaatgcatt
     2641 taacctacat aaaaatacat gatcaacata tgttttatgc tgattacaac taaatcaaac
     2701 gtaatgaaat taatttataa aagcgattta aatatgtcaa tattttaagt taaaatcact
     2761 tgaaacctac tataaatgaa cttaaattta aaaaatctcc tttaattcca atgcagcact
     2821 gttgtttttt cagctgagga cccccggtgc agatggttgg cccccggccc gctattctaa
     2881 ggaagccttt cttaatttta gggcggcgct gcatgcgctc agatcagatt cacgggtgga
     2941 gagggttatt ttctcttttt tattttttct tgcctgctca tatttgggcc tgtgtgaacc
     3001 ccaggctccg cctcggcccc caggagccgc tcttatttaa aggggatcct cgccttcagt
     3061 cttcgtccca gtggaagcat cagtcttgtg ataacaactt ggtgagtcct acagaccagc
     3121 gctcgactct aggctttaaa tgcattgatt tctctggatt agctaccggg agagttttaa
     3181 ctttgcttat tagtcttgtg cttgaactgc taggtttaaa gagggttttc tctaatgcat
     3241 gcatgaagcg acagctgaaa accagaaata gagcgtttgt cctcctgtta aactctagcc
     3301 tttctttggc atttgtcagt ccgagtcttc agctgccggc aggagattgg gtttcagtaa
     3361 cacttgctga gcacaaacta cacgtgcttc cttcagattt aattcattat taatcattct
     3421 agatttaaat gatcttctta ctcattgctg attattttag ccttgcggag cagattaaat
     3481 gagcacgtgt ttattaagat tgatttaaat ataattaata tttcaccact gtagttttaa
     3541 aattgccttc taagtcatgg ttgattttta ataggtatcg gctctaatta gattactatt
     3601 ttggttttta aatgttattt ctatttcatt tgtaggctcg tttttaatgt gattttatta
     3661 gattctagtt ttaaaatcat cttttagcct tactgagcag actgaatgaa catgtttcat
     3721 taagattaac ttcattatag ttaattattt aatcattcta gtggattata aatcagctct
     3781 atttaaattg attgtcgtgt ttatttctat ttgatttttg attatttttt aaggtcgttt
     3841 ttatgtgatt tttccccgat tctaatttta aactaattgt ttgattttta tatgattcga
     3901 ctctttttaa atgaattgtt ttgctttttt tattgagcat atatatatat tatacaattt
     3961 caatcaacaa ttgttgtgct ttttaaatgt tgtttctatt tcatttctag ggtcgatttt
     4021 attcgattct agttttaaaa tcgtgatttc taactcctaa ccttactgag cagactgaat
     4081 gaacatgttt catcaagatt aacttcatta tagttaatta ttgaatcggg ctagtggttt
     4141 ataaatcagc tctattataa attaattgtc atcattattt ctatttaatt tttattattt
     4201 ttttaggatt atttttaagg gatttttttc acgttctagt tttaaactca atgctcatta
     4261 ctgctcaccc ttgctgagca gtctttcgtt aagattaaat taattaataa cccattctag
     4321 ttttaaaaca gtgtcttaag gcatagctga tattttacaa gtttactatt taatatatct
     4381 ccatttcttt ttttagggtc gatttcatct gatttgatta atttttatta tcttcactaa
     4441 gctttttgtt ctaggtttta ttctcagtat tattactgtt atatttgaac tgaatttctc
     4501 ctcatagttt attcatttta tttatttatt ttttgtttat tcgctatttt gtattttagt
     4561 ttttaattag aaattttatt agaatcgttt tgaactagtt tttatgcact ctaacattaa
     4621 tattaaatca taactattat ttttattata atttatttgt gtttatcttt ttgtgtagct
     4681 ttcatgtgtt tttattttag ttttattatg tttgcattga attttttgga ctagtttata
     4741 taacgatata cagaaaagcc atgacaacaa atgtttttat aatacctatt aagtataagc
     4801 taatcagatt tccactattc tgttcaaagt taacattatt agccattctg atggatcatt
     4861 tgacatttgt catatgtctt tatgaacact gctctttcag atgccacttt taaagctgtt
     4921 ttttacagta aatgaacgtt tattattatg acattatttc ctaaggcctc tagagtacag
     4981 caggatgtgg cgtgtgcatt actgtgtaat tatgctgact gtaaatgtgc atttatagac
     5041 tgtcagcatg tctgactgtg cgctgttgtg atttgtcctg cacagaccaa ggcaagtttg
     5101 tacaaaaaag caggctgcca ccatggtgag cgagctgatt aaggagaaca tgcacatgaa
     5161 gctgtacatg gagggcaccg tgaacaacca ccacttcaag tgcacatccg agggcgaagg
     5221 caagccctac gagggcaccc agaccatgag aatcaaggcg gtcgagggcg gccctctccc
     5281 cttcgccttc gacatcctgg ctaccagctt catgtacggc agcaaaacct tcatcaacca
     5341 cacccagggc atccccgact tctttaagca gtccttcccc gagggcttca catgggagag
     5401 agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca gcctccagga
     5461 cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcccatcca acggccctgt
     5521 gatgcagaag aaaacactcg gctgggaggc ctccaccgag accctgtacc ccgctgacgg
     5581 cggcctggaa ggcagagccg acatggccct gaagctcgtg ggcgggggcc acctgatctg
     5641 caacttgaag accacataca gatccaagaa acccgctaag aacctcaaga tgcccggcgt
     5701 ctactatgtg gacagaagac tggaaagaat caaggaggcc gacaaagaga cctacgtcga
     5761 gcagcacgag gtggctgtgg ccagatactg cgacctccct agcaaactgg ggcacagaga
     5821 cccagctttc ttgtacaaag tggtcgactt aagacgaata ttacagatga ctcctgggaa
     5881 aggaggctct cagagctcgg agtctgagca gccgtctgct gatatgaata atgagtcctc
     5941 agagggcttt atttgtcccc agtgtatgaa atcccataac tctgctgagg agctcttcaa
     6001 acattatgag gtctttcatg aaccaggaga ccagccgtcc ccctttagcc ccggcaggga
     6061 ggatttaaca ttacttcgac aggaaatcca agacttacag gcttcactga aggaggaacg
     6121 ctggttttct gaggaattga aaaaagaatt agacaaagta caaggacgaa tagcaactaa
     6181 taaaggctta cagagcgatg gtacaagtgg agaagatgca gagctcgaga tgagactcaa
     6241 tgaatcggag acagagaagt tcaacatcaa acagatgaag gacctgtttg aacaaaaagc
     6301 tgctcagctt gcgaccgaga tagttgacat caaatctcgc tacgatgagg agaaaagtat
     6361 gagagaagct ttggagcaga gactggccaa cttaaaccaa gatatgcaaa aagaaaaaca
     6421 ggagaaggag aaactgtcgg cagagctgct ccagaggccg ggtgtggaag atgtggaggt
     6481 gttgaagaaa gagctggtgc gggtgcagac gctcatggac agcatgaccc gagagcgaga
     6541 ggaggagtca gagcgcctca agggccaata tgagcagctg caggccaact tcactacctc
     6601 agagaaaacg attgctcaat taaaggctga actggagaag ggtcctcaag aagcagcggt
     6661 ttacacacaa cagatccacc aattacagag cagcttgaac aacctccagc agcaatccca
     6721 ggctttgtca gagaaattat ctcgtaaaga gaaggagaat caggaactgg aggagcgtct
     6781 gggtcacgaa caggcctcta agaagagtct tcaggctaat ctgcatcaga aagagctgga
     6841 gctccaggag agtcgagcac gggtgtcttc aggagaagca gctctcagtc gagcacaggc
     6901 tgaattaact gaacgtgggg aagaggctgc acgactcaga cgagagctgg gcgagctgga
     6961 gaagaaccag caggagctga aggcagagcg gaaacaactt caacagcaga gggaggataa
     7021 agaaaaccag ggcctgcagc aacagagtga gatcagccag ctgcatgcga agctgctgga
     7081 agcggagcgt caggtggggg agctgcaggg tcgtctgaaa gagcagagac agctgtctgg
     7141 agagaagctc aaagacagag aacagcaggc agcagatctc cagctcaaac tctcccgctt
     7201 agaggaagag ttaaaggaga gctccaccaa gtccactgac ctgcagcatc agctggacaa
     7261 atccaaacag cagcatcaag aactacaaac acttcaacag agcactaatg gcaaacttag
     7321 agaagcacag aatgacctgg agcaggtgtt gcgtcagatc ggcgacaaag accagaagat
     7381 ccagaatctt gaagctctgc tccagaagag taaggaaagt gtgagccagc tggagaccga
     7441 gagagaggat ctgtgtgcca agattcaggc tggagaagga gaagctgctt tgctgaacca
     7501 attacaagag aaaaatcact cgctacaaga acagatcacg cagcttacag acaagcttaa
     7561 gaaccagtcg gagagccaca agcaggctca ggacaacctt catgaacagg tgcaggagca
     7621 gaagacacac ctgcgttcag ctcaagaccg ctgtcagggt ctggagacca ccgtcactga
     7681 actcaacaca cagctcacag agagcagaga gaagatcgct cagctagaca ctcagttgaa
     7741 agccaagact gagatgctgt tgtctgctga agcagcaaaa aatgcccaga gagctgactt
     7801 ggagagccac ctagagactg ctcagaatgc attgcaggat aagcagcaag agctcagtaa
     7861 agtgcaggct cagctggagg agcaggcccg caggctgacg gagaagcagg agcagtgctc
     7921 tcagctggag aacggcctga aggacagcag agacaaactg atgactgcag aacagaggat
     7981 agagacgctc cagacccaga ccaagaaagc cgaggttgag ttgactgagc tccgctccgg
     8041 aagagaacag gctcagaaag agcagtcttc tcttaagaag cagatcagtg agctggagat
     8101 gaagaccaaa gagttgaacc gacttttaga ttccgagaaa caaggagctt ccacccagca
     8161 agaggagcta aagaagaaaa gctcagctct caccgaaaca agacaaaagc tggagaaagc
     8221 agagcaggag cgtggagccc tgcaggccaa cctggataaa ctgtctcagg agggtcagaa
     8281 gcagcaggca gagttggaca agaaggtgca gggtctgagt tcagatctcc agaaaactca
     8341 gggggagaaa gaagcgctgg taaaagaagt ggcgacagtg aaagaggctc tgagtaaagc
     8401 ttctaaagcc ctgaaggaga gccagactca attagacaag gagaagaagg gcagcaagac
     8461 cactttggag gagaaggaaa aggtttttca aaaagtacaa caagagcttc agaagaactc
     8521 agaggccact tctaaagagc tcagcaatgt caaaggtctg ctggagaaat cgatagaggc
     8581 agaaaagagt cttcaatctc agctgactgc actgaacgct cagctgaagc agtgtcagga
     8641 tacactgaag gagaaagaaa agagtgaaca gcagctgcag gcagagctga agaccaggca
     8701 aggctccttc agtcaggaag tgaagaaact gaagacacag gtgtctgagc tgcaggcttc
     8761 ccttgctaaa aagactgaag aggaagccaa gcaaaaggag caaatcacag cactgtctaa
     8821 agatctgagc tcagagaaga atcgctgtgc agagctgcag aaaaccagtg acggcaccaa
     8881 agagagtctc accgcccttc agtctgatta ctatggcaaa gagtctgagc tctctgctgt
     8941 acgccaggac cttaaggtgt gcgaggagaa gttggttctt gctcaggagg agctggtgac
     9001 caacaggaac cagctcagtg cttacgaaac ccaaatccag gagctgaaga cgggccacac
     9061 agcgatggag atagacctga gcaagagaga cgagaagatc aaacagcaag tggagactct
     9121 gcagaagctc cagaaacaac agggacaaac agaggagcag ctgaagaagg agaaagctca
     9181 atgcgaggag ctgagagaga gtcaaagcgc cctggaaaag gacaaaaaca aactgtctgc
     9241 tgatctcaaa acactggctg agaagaatga gaaggagctg aaggacttgc aggcagcaaa
     9301 gcagttgttg atccagcaaa aggtggagat gcaggggaag gtagaggcgg ctcaagcatc
     9361 tgtggagaaa gaacagagag agcatcagaa gaccagagac tccatcaaac agaatgagca
     9421 gcagctcaag gctgagacca acaaaataaa gcagcagctg gcatcagaag tgaaggctaa
     9481 agaggagatg gtgcggcaga gggaagaggc tgaggtgaag atgtccatgc aggtgacggc
     9541 actgaatgag aacgtggcca cgctgaagcg agagtggcag agcagccaga ggcgggtggg
     9601 tgagctggag aaacagacgg atgagctgag aggagagatt gccgtgctgg aagcaacagt
     9661 ccagaacaac caagacgaaa gacgtgcact acttgagagg tgcgtccagg gtgaagggga
     9721 gatggagaag cttcaggcta agatgttgga gatgcgcagg aagttggacg acactactgc
     9781 cgccatgcag gagcttggtc gagagaacca gtccttacag ataaaacagt cacaatccct
     9841 cacacggaag tggacagaag accatgaggt gcagaactgc atggcttgtg ggaaaggctt
     9901 ctccgtcact gtcagaaagc accactgcag acactgcggg aacatcttct gtgcagagtg
     9961 ctctgcccgc aacgccctca caccctcctc caagaagccc gttcgagtgt gtgacaactg
    10021 ctttgatgag ctccagggct gactagtcaa ctttattata catagttgat aattcactgg
    10081 ccgtcgtttt acggtaccat cgatgatgat ccagacatga taagatacat tgatgagttt
    10141 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
    10201 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
    10261 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
    10321 tacaaatgtg gtatggctga ttatgatcct ctagatcaga tctgcgaaga tacggccacg
    10381 ggtgctcttg atcctgtggc tgattttgga ctgtgctgct cgcagctgct gatgaatcac
    10441 atacttcctc cattttcttc cactgattga ctgttataat ttccctaatt tccaggtcaa
    10501 ggtgctgtgc attgtggtaa tagatgtgac atgacgtcac ttccaaagga ccaatgaaca
    10561 tgtctgacca atttcatata atgtgaaaac gattttcata ggcagaataa ataacattta
    10621 aattaaactg ggcatcagcg caattcaatt ggtttggtaa tagcaaggga aaatagaatg
    10681 aagtgatctc caaaaaataa gtactttttg actgtaaata aaattgtaag gagtaaaaag
    10741 tacttttttt tctaaaaaaa tgtaattaag taaaagtaaa agtattgatt tttaattgta
    10801 ctcaagtaaa gtaaaaatcc ccaaaaataa tacttaagta cagtaatcaa gtaaaattac
    10861 tcaagtactt tacacctctg gttcttgacc ccctaccttc agcaagccca gcagatccac
    10921 tagttctaga gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt
    10981 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
    11041 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
    11101 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
    11161 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
    11221 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
    11281 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
    11341 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
    11401 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
    11461 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
    11521 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    11581 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
    11641 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    11701 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    11761 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    11821 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
    11881 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    11941 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    12001 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    12061 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    12121 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    12181 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    12241 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    12301 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    12361 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    12421 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    12481 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    12541 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    12601 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    12661 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    12721 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    12781 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    12841 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    12901 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    12961 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    13021 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    13081 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    13141 cccgaaaagt g
//
