LOCUS Exported 4141 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Multisite gateway vector for 3' tagging with yeast ATG18 (PLEKHA3). Binds PtdIns(3,5P)2. ACCESSION . VERSION . KEYWORDS p3E-ATG18 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4141) TITLE Parton Lab Plasmids for in vivo cell biology in the zebrafish REFERENCE 2 (bases 1 to 4141) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4141 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind complement(624..748) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(2250..2345) /gene="mutant version of attL" /label=attL3 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2382..2401) /label=T7 /note="T7 promoter, forward primer" promoter complement(2383..2401) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2406..2422) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2406..2422) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2524..2543) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2535..3344 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2602..2621) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3491..4079 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3980..3999 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac 601 taataccatc taagtagttg gttcatagtg actgcatatg ttgtgtttta cagtattatg 661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt 721 ctcgttcaac tttcttgtac aaagtggtgt ctgattcatc acctactatc aactttatta 781 atttcaatca aaccggaacg tgtatttccc ttggaacgtc gaaaggtttc aaaatattca 841 attgtgagcc cttcggaaaa ttttattcag aggacagtgg gggctatgct atcgtcgaga 901 tgttgttctc cacctcgtta ctagccctcg ttgggatagg cgatcaacct gcgctttcac 961 caaggagatt gcgtataatc aacacaaaaa aacattctat tatctgtgag gtgactttcc 1021 ctacttctat tctgagtgtg aaaatgaata agtctcgatt ggtggtactt ttacaagagc 1081 agatttatat ttatgatatc aacaccatga gactattgca tactatagaa acaaacccta 1141 acccacgtgg ccttatggct atgtctcctt cggtagccaa cagctattta gtgtatccat 1201 caccaccaaa agttattaac tccgaaatta aagctcatgc caccacaaac aatatcacat 1261 tgtcagttgg tggcaacaca gagaccagtt tcaagagaga tcagcaagat gctggccata 1321 gtgacattag cgacttggat cagtattcga gctttactaa gagggatgat gcggatccaa 1381 caagcagtaa cggcggtaac agcagtataa taaagaatgg tgatgtgatc gtattcaact 1441 tggaaacatt acagccaacc atggtcatcg aagctcataa gggcgagatt gctgcaatgg 1501 caattagttt tgatgggaca ctaatggcta ccgcctctga taaaggtact atcatcaggg 1561 tctttgacat tgaaacgggt gataagatct accaattcag gagagggacg tacgcgacaa 1621 gaatttactc catatcattc agtgaagata gccagtactt ggcggttacc ggctcttcca 1681 aaaccgtgca tatcttcaaa ttggggcatt caatgagcaa caataaacta gacagcgatg 1741 atagcaacat ggaagaagct gcagccgatg attcatcgct cgataccacc agtatcgatg 1801 cgctgagtga cgaggaaaac ccgacaagac tcgcaagaga accatatgtg gatgcatcaa 1861 gaaagacaat gggtaggatg atacgttact cttctcaaaa gctatcccga agagctgcca 1921 gaacattggg tcagattttc cccatcaaag ttacatcgtt gttggaatcc tcgcgccatt 1981 ttgcgtcttt gaaacttccc gttgaaacca attcccatgt aatgaccata tcaagtatag 2041 gctctccaat agatatagac acatccgagt atccggaact cttcgaaact ggcaattccg 2101 caagtacaga gtcctaccat gagcctgtta tgaagatggt ccccatcagg gtcgtttcct 2161 cggatggata cctatacaac tttgttatgg acccggagag aggcggcgat tgcttaatat 2221 tgtcacagta ttccatcttg atggattgac aactttatta tacaaagttg gcattataaa 2281 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat 2341 tatttggagc tccatggtag cgttaacgcg gccgcgatat cccctatagt gagtcgtatt 2401 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca 2461 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta 2521 atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca 2581 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg 2641 cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca 2701 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat 2761 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca 2821 ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg 2881 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta 2941 attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata 3001 acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag 3061 tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg 3121 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg 3181 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg 3241 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata 3301 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt 3361 ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa 3421 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca 3481 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 3541 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 3601 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag 3661 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 3721 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 3781 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 3841 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc 3901 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 3961 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 4021 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 4081 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 4141 t //