LOCUS Exported 3733 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Multisite gateway vector for 5' tagging with Bin1b. ACCESSION . VERSION . KEYWORDS p3E-Bin1b SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3733) TITLE Parton Lab Plasmids for in vivo cell biology in the zebrafish REFERENCE 2 (bases 1 to 3733) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3733 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind complement(624..748) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1842..1937) /gene="mutant version of attL" /label=attL3 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1974..1993) /label=T7 /note="T7 promoter, forward primer" promoter complement(1975..1993) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1998..2014) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1998..2014) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2116..2135) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2127..2936 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2194..2213) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3083..3671 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3572..3591 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac 601 taataccatc taagtagttg gttcatagtg actgcatatg ttgtgtttta cagtattatg 661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt 721 ctcgttcaac tttcttgtac aaagtggtgg cagaggttgg gaaaggagtc aacgccggga 781 aactggccag taatgtacag aagaggttaa cccgagcaca agagaaggtg atgcagaaac 841 tgggaaaggc ggatgaaacc aaagacacag cctttgagga gcaagtagcc aactttaaca 901 aacagatgaa tgagggcacc aaactgcaga aagacttgaa agcgtatttg ctggcagtca 961 aaacaatgca cgagtgttcc aagcgtctgc acgactgtct ggcagaaatg tatgaccctg 1021 agtggtttgg caaagaggag gtggactcca tcgcagagga cactgacctc ctgtggcaag 1081 atttccacga gaagcttttg gacaatgctg taatttccat ggacgcatac ttggctcagt 1141 ttccagatat aaaggctcgc attgcaaagc gtgaaaggaa gctggtggac tttgacagtg 1201 ccagacatca ttttgcctct gtacaaaaag gcaagaaaaa ggacgaggcc aaaattgcca 1261 aggctgagga acacttgggc agagctcaga aagtgtttga ggagatcaat tatgatctcc 1321 aggaggaact tcccaccctg tggaacagtc gtattggcct ctatgttaac acattccgga 1381 gtgtgtctgg cctggaggaa acatttcaca gggacatggg caaactgaac cagaatctga 1441 gtgatattat gaccaaactg gaagagcagc gtgttgccaa aaaaggtgtc acgacagcaa 1501 gcactgggaa gagtgacgat gcagcaaacc acaatcagga gacgtcagat gcacctaaat 1561 ctccatcaaa gaatgaagcc ccaacggtga caaatggctc ctcagataca gagcttcctc 1621 catctgttct ctttaaggtg aaaacaatgc acgactacag tgcaacagac agtgatgaac 1681 ttgacctgaa ggctggtgac attgtgcttg tgcttccctt tgccaaccca gatgaagcgg 1741 atgatggctg gttaatgggt gtaaaagagt cccactggct tcagaacaaa aacctcctag 1801 caaaaggagt cttccctgaa aacttcaccc agaaactgtg acaactttat tatacaaagt 1861 tggcattata aaaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca 1921 aaataaaatc attatttgga gctccatggt agcgttaacg cggccgcgat atcccctata 1981 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc 2041 tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt 2101 acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc 2161 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg 2221 ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc 2281 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact 2341 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg 2401 catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc 2461 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga 2521 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat 2581 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc 2641 ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg 2701 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt 2761 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga 2821 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg 2881 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag 2941 aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa 3001 gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg 3061 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 3121 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 3181 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 3241 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 3301 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 3361 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 3421 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag 3481 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 3541 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 3601 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 3661 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 3721 ttgctcacat gtt //