LOCUS Exported 5200 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Multisite gateway vector for 3' tagging with Dynamin2b. ACCESSION . VERSION . KEYWORDS p3E-Dnm2b SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5200) TITLE Parton Lab Plasmids for in vivo cell biology in the zebrafish REFERENCE 2 (bases 1 to 5200) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5200 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind complement(624..748) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(3309..3404) /gene="mutant version of attL" /label=attL3 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(3441..3460) /label=T7 /note="T7 promoter, forward primer" promoter complement(3442..3460) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(3465..3481) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3465..3481) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(3583..3602) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 3594..4403 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(3661..3680) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 4550..5138 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5039..5058 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac 601 taataccatc taagtagttg attcatagtg actgcatatg ttgtgtttta cagtattatg 661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt 721 ctcgttcaac tttcttgtac aaagtggtgg ggaaccgcgg gatggaggac ttgattcccc 781 tcattaacaa gctgcatgac gccttcagct ccatcggcca gagctgcaac ctggagctgc 841 cgcagatcgc ggtggtcggc gtgcagagtg ccggcaagac ctccgtgctc gagaacttcg 901 tgggaaggga ctttctgccg agaggaccag gaatcgtcac tcgcaggcct ctgattctcc 961 agctggtgaa cagtaaagct gaatatgccg agttcctgca ctgtaagggc cgcaagtttg 1021 tggactttga cgaggttcgg atggagattg aagcagaaac agaccgaatc actgaatcta 1081 acattggaat ctctgcagtg cccattaacc tgcgccttta ctcgcccaac gtgctgaatc 1141 tgacgctgat cgatcttccg ggcataacga aggtggcggt gggtgaccag ccgcaggata 1201 tcgagtacca gatccgggat atggtgttgc agttcatctc caaagagaac tgtctgattc 1261 tggccatcac tccagcaaac atggacctga ccaactctga cgctctcaag atcagcaaag 1321 aggtggaccc tcatggtctg cgcaccatcg gtgtgatcac caaactggac ctgatggatg 1381 aaggaaccga cgccagagac atcctggaga acaagctgct gccgctgcgc agaggttata 1441 tcggtgtggt gaaccgcagt cagaaggaca ttgatggcag gaaggacatc cgcgcggctc 1501 tttctgctga gaggaagttc ttcctgtccc atccagcata cagacacata gcagagagaa 1561 tgggcacacc gtacctgcag aaaacactca accagcaact gatgaaccac atccgcgaca 1621 cactgccggc gctgcgcagt aaactgcagt ctcagctgct gtcgctggag aaagaggtgg 1681 aggagtacaa gaacttcaaa ccagacgatc ctgcgcgcaa aaccaaagcc ctgctgcaga 1741 tggtccagca gttcggtgtg gactttgaga agcgtatcgg gggctctgga gatcaggtgg 1801 acactctgga actctccggc ggagctcgaa taaaccgcat cttccacgag cgcttcccct 1861 tcgagctggt gaagacggag tttgatgaga aggagttgcg gagggagatc agttacgcca 1921 tcaagaacat tcacggagtc aggacggggc tcttcacccc agatctggcc ttcgaggcca 1981 tcgtgaaaaa gcagctgata aagctgaagg agccgtgcct caagtgcatc gacctggtca 2041 tccaggagct gatcaacacg gtcaggcaga gcacactgaa gctgaagttg tatcctcggc 2101 tgagagaaga gacggagaga atcatcacaa tatatgtcag agagagagag accaaaacca 2161 aagaccagat gatgctgctg atcgatgtcc agctctccta catcaacaca aaccacgagg 2221 acttcatcgg attcgccaat gcgcaacaga gcagcagaga tgtcactaag aagcaagccg 2281 ttcccaatca ggtgatccgc cgtggttggc tgaccatcaa catcagcatc atgaagggcg 2341 gctccaaaga ctactggttc gtgctgacgg cggagtctct gtcctggtac aaggatgagg 2401 aggagaagga gaagaagttc atgctgccgc tggataacct gaagctgcgg gatgtggaga 2461 agagcttcat gagcaacaaa cacaccttcg gcatcttcaa caccgagcag aggaacgtgt 2521 ataaagacct gcgtcagatc gagctggcct gcgccactca ggaggatgtg gacagctgga 2581 aggcctcttt cctcagagcg ggagtctatc ctgagaaaga ccaggtggag aacggggacg 2641 tgtcagcaga ctttctctcc atggaccctc agctggagcg tcaggttgag accatcagta 2701 atctggtgga ctcgtacatc agcatcgtca acaagagcat ccgagacctg atgcccaaaa 2761 ccatcatgca cctgatgatc aacagcacga aggagttcat ctactcggag ctgctggcgt 2821 acctgtactc gtcaggtgat cagaacagtc tgatggagga gtcggcggag caggagcagc 2881 ggcgggacga gatgctccgc atgtacaacg ctctgaaaga ggctctgaag atcatcgggg 2941 acgtcagcac atccaccttc accacacctg tgcccgcgcc ggtggacgac acctggatac 3001 acactgaccc cagtcctccg tctcagcgta agccagctcc gcccactgtc cgccctcctg 3061 cagtgcgagg ctccaccccc ggtgctccag ccccaccttc tctaaacccc tcccctgcct 3121 tcggagctcc gcctgttccc tccagaccca ccacattgga gccagctgta gctccgcccc 3181 cttgtgttcc atctcgccct gcacaggtcc cgcccactgt gctgcccggg atacgcagta 3241 ggaggcctcc agcggctcca aacaggccca caatcatccg tccgacagaa ccatctctgc 3301 tggactgaca actttattat acaaagttgg cattataaaa aagcattgct tatcaatttg 3361 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttggagct ccatggtagc 3421 gttaacgcgg ccgcgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct 3481 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat 3541 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc 3601 atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg 3661 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg 3721 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg 3781 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca 3841 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa 3901 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg 3961 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc 4021 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg 4081 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac 4141 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta 4201 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc 4261 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga 4321 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt 4381 tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca 4441 ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg 4501 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt 4561 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt 4621 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc 4681 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg 4741 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg 4801 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt 4861 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac 4921 tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg 4981 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg 5041 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat 5101 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt 5161 tacggttcct ggccttttgc tggccttttg ctcacatgtt //