LOCUS Exported 3217 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Multisite gateway vector for 3' tagging with dominant negative Rac1. ACCESSION . VERSION . KEYWORDS p3E-Rac1-DN SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3217) TITLE Parton Lab Plasmids for in vivo cell biology in the zebrafish REFERENCE 2 (bases 1 to 3217) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3217 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind complement(624..748) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1326..1421) /gene="mutant version of attL" /label=attL3 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1458..1477) /label=T7 /note="T7 promoter, forward primer" promoter complement(1459..1477) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1482..1498) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1482..1498) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1600..1619) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1611..2420 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1678..1697) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2567..3155 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3056..3075 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac 601 taataccatc taagtagttg gttcatagtg actgcatatg ttgtgtttta cagtattatg 661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt 721 ctcgttcaac tttcttgtac aaagtggctc aggccataaa gtgtgtggtc gtcggggacg 781 gggctgtggg aaaaaactgc cttctgatca gctacaccac caatgctttt cctggggaat 841 acattcccac tgtgtttgat aattattctg caaatgtgat ggtcgatggg aaaccagtaa 901 acctgggatt gtgggataca gcaggacaag aggattatga caggcttcga ccgctttcct 961 accctcagac ggatgtgttc ctgatctgct tctctcttgt gagtccggcc tcatttgaaa 1021 acgtccgtgc aaagtggtat ccggaggtca gacatcattg ccctaacact ccaataattc 1081 ttgttgggac taagcttgat ttgcgagatg acaaggacac catcgaaaag ttaaaggaga 1141 agaaactcac tcccatcacc taccctcaag gccttgctat ggccaaagaa ataggagctg 1201 tgaagtacct ggagtgctct gccttaaccc aacgcggcct taaaactgtg ttcgatgaag 1261 ccatccgtgc agtgctgtgt ccacctccgg tgaagaggag gaggagaaga tgtctccttc 1321 tgtgacaact ttattataca aagttggcat tataaaaaag cattgcttat caatttgttg 1381 caacgaacag gtcactatca gtcaaaataa aatcattatt tggagctcca tggtagcgtt 1441 aacgcggccg cgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc 1501 agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat 1561 catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata 1621 ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt 1681 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga 1741 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta 1801 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc 1861 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag 1921 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag 1981 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg 2041 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt 2101 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt 2161 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt 2221 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat 2281 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac 2341 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga 2401 tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta 2461 cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt 2521 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 2581 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 2641 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 2701 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 2761 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 2821 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 2881 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 2941 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 3001 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 3061 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 3121 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 3181 ggttcctggc cttttgctgg ccttttgctc acatgtt //