LOCUS Exported 3181 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Multisite gateway vector for 3' tagging with dominant negative ARF1. ACCESSION . VERSION . KEYWORDS p3E-ARF1-DN SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3181) TITLE Parton Lab Plasmids for in vivo cell biology in the zebrafish REFERENCE 2 (bases 1 to 3181) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3181 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind complement(624..748) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1290..1385) /gene="mutant version of attL" /label=attL3 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1422..1441) /label=T7 /note="T7 promoter, forward primer" promoter complement(1423..1441) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1446..1462) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1446..1462) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1564..1583) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1575..2384 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1642..1661) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2531..3119 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3020..3039 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac 601 taataccatc taagtagttg gttcatagtg actgcatatg ttgtgtttta cagtattatg 661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt 721 ctcgttcaac tttcttgtac aaagtggtgg gaaacatatt cgcaaacctc tttaaagtct 781 tcggcaagaa ggagatgaga attcttatgg tgggactcga cgcagcaggg aagaacacga 841 ttctgtataa attaaagttg ggggaaattg tgaccaccat cccaacaatc ggttttaatg 901 ttgaaacagt ggagtacaag aatattagtt ttacagtttg ggacgtcggc ggtcaagata 961 agattcgacc attgtggcgt cattatttcc agaacacaca aggtctgatc tttgtggttg 1021 acagtaatga ccgtgagcgt gtgaatgagg cgcgagagga gctgatgagg atgttggctg 1081 aggatgagct cagagaagca gttctgctgg tgtttgccaa caaacaggac ctgccgaacg 1141 ccatgaacgc cgccgaaatc acggacaagc tgggtctgca ctcactccgg cacagaaact 1201 ggtacattca ggccacgtgt gccacaagcg gcgacggcct ctatgaagga ctcgactggt 1261 tgtccaatca gctgaaaaac cagaaatgac aactttatta tacaaagttg gcattataaa 1321 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat 1381 tatttggagc tccatggtag cgttaacgcg gccgcgatat cccctatagt gagtcgtatt 1441 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca 1501 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta 1561 atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca 1621 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg 1681 cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca 1741 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat 1801 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca 1861 ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg 1921 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta 1981 attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata 2041 acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag 2101 tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg 2161 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg 2221 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg 2281 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata 2341 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt 2401 ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa 2461 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca 2521 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 2581 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 2641 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag 2701 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 2761 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 2821 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 2881 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc 2941 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 3001 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 3061 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 3121 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 3181 t //