LOCUS Exported 3166 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Multisite gateway vector for 3' tagging with dominant negative ARF6. ACCESSION . VERSION . KEYWORDS p3E-ARF6-DN SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3166) TITLE Parton Lab Plasmids for in vivo cell biology in the zebrafish REFERENCE 2 (bases 1 to 3166) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3166 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind complement(624..748) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1275..1370) /gene="mutant version of attL" /label=attL3 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1407..1426) /label=T7 /note="T7 promoter, forward primer" promoter complement(1408..1426) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1431..1447) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1431..1447) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1549..1568) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1560..2369 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1627..1646) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2516..3104 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3005..3024 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac 601 taataccatc taagtagttg gttcatagtg actgcatatg ttgtgtttta cagtattatg 661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt 721 ctcgttcaac tttcttgtac aaagtggctg ggaagatgct ttccaagatc ttcggcaaca 781 aggagatgag aatattgatg ctcggacttg acgccgccgg aaagaacacg attctgtaca 841 aactcaaact gggccagtcg gttacgacca tcccgacagt gggcttcaat gtagagactg 901 tcacgtataa aaacgtcaag tttaacgttt gggacgtcgg cggtcaggat aagatccgtc 961 ctctatggcg gcactactac accggcacgc agggcctgat tttcgtagtg gactgtgcgg 1021 atcgtgaccg catcgatgag gctcggcagg agctgcaccg cattatcaac gatcgtgaga 1081 tgcgcgacgc cattatcctg atcttcgcca acaaacaaga ccttccagac gccatgaagc 1141 cgcatgagat acaggagaag ctgggcctga cgcgcatccg agatcgcaac tggtacgttc 1201 agccgtcttg cgctacaacc ggcgacggac tctacgaagg gctcacctgg ctcacgtcca 1261 actacaaatc ctaacaactt tattatacaa agttggcatt ataaaaaagc attgcttatc 1321 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt ggagctccat 1381 ggtagcgtta acgcggccgc gatatcccct atagtgagtc gtattacatg gtcatagctg 1441 tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa 1501 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta 1561 tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt 1621 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct 1681 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca 1741 atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga 1801 ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg 1861 gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg 1921 cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca 1981 gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg 2041 cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc 2101 ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata 2161 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg 2221 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat 2281 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt 2341 ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg taacactggc 2401 agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga 2461 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 2521 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 2581 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 2641 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga 2701 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 2761 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 2821 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 2881 ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa 2941 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 3001 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 3061 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 3121 cctttttacg gttcctggcc ttttgctggc cttttgctca catgtt //