LOCUS Exported 3302 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Multisite gateway vector for 3' tagging with rab11ba. ACCESSION . VERSION . KEYWORDS p3E-rab11ba SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3302) TITLE Parton Lab Plasmids for in vivo cell biology in the zebrafish REFERENCE 2 (bases 1 to 3302) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3302 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind complement(624..748) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1411..1506) /gene="mutant version of attL" /label=attL3 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1543..1562) /label=T7 /note="T7 promoter, forward primer" promoter complement(1544..1562) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1567..1583) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1567..1583) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1685..1704) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1696..2505 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1763..1782) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2652..3240 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3141..3160 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac 601 taataccatc taagtagttg gttcatagtg actgcatatg ttgtgtttta cagtattatg 661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt 721 ctcgttcaac tttcttgtac aaagtggtgg ggacccgtga cgacgaatac gattatttat 781 ttaaagtggt tctcattgga gactctgggg tggggaagag taacctgttg tcccgtttca 841 ctcggaacga gtttaacctg gagagcaaga gcaccattgg tgtggagttc gcaacccgca 901 gcatccaagt ggacgggaag accattaaag ctcagatctg ggatacagca ggacaggaac 961 gctacagagc aataacatca gcgtactacc ggggtgcagt tggagctctt ctggtctacg 1021 acatcgcaaa acatctgact tacgagaatg tggagcgctg gctgaaggag ctgcgggatc 1081 atgcggataa caacatcgtc atcatgctgg tgggcaacaa gagcgacctg aggcacctga 1141 gggccgtgcc cactgatgag gccagagctt ttgcagagaa gaacaatctc tccttcattg 1201 aaacatcggc gttggactcc acaaatgtgg aggaggcatt taaaaacata ctaacagaaa 1261 tctatcggat tgtgtcacag aaacagatgg tcgacaggcc cgggcacgat gagtctcctg 1321 gcaataacgt agtggacatc agcgtgccgc ccactactga cgggttaaag ggcaacaaat 1381 ttcagtgctg tcagaacctg tgacccaaca caactttatt atacaaagtt ggcattataa 1441 aaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca 1501 ttatttggag ctccatggta gcgttaacgc ggccgcgata tcccctatag tgagtcgtat 1561 tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac 1621 attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt 1681 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc 1741 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt 1801 gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc 1861 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa 1921 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc 1981 accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt 2041 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt 2101 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat 2161 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa 2221 gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt 2281 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt 2341 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt 2401 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat 2461 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat 2521 tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca 2581 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc 2641 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 2701 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 2761 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta 2821 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 2881 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 2941 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 3001 gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg 3061 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 3121 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 3181 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 3241 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 3301 tt //