LOCUS Exported 3259 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Multisite gateway vector for 3' tagging with rab1ba. ACCESSION . VERSION . KEYWORDS p3E-rab1ba SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3259) TITLE Parton Lab Plasmids for in vivo cell biology in the zebrafish REFERENCE 2 (bases 1 to 3259) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3259 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind complement(624..748) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1368..1463) /gene="mutant version of attL" /label=attL3 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1500..1519) /label=T7 /note="T7 promoter, forward primer" promoter complement(1501..1519) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1524..1540) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1524..1540) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1642..1661) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1653..2462 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1720..1739) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2609..3197 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3098..3117 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac 601 taataccatc taagtagttg gttcatagtg actgcatatg ttgtgtttta cagtattatg 661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt 721 ctcgttcaac tttcttgtac aaagtggtga atcctgaata tgactacctg ttcaagttgc 781 tcctcatcgg tgactctgga gtgggaaagt cgtgtctgtt gttgagattt gctgatgaca 841 catacactga gagctatatc agcaccatcg gcgtggactt caagatccgc accattgaac 901 ttgatggcaa aaccattaag ctacagatat gggatactgc agggcaagaa agatttcgaa 961 ctatcacctc cagttactat cgaggggctc atggaatcat tgtggtttat gatgtgacgg 1021 atcaggaatc gtataacaat gttaaacagt ggcttcagga aatagaccgc tacgcaagcg 1081 agaatgtcaa caagctgctg gtggggaaca aatgcgacct caccaccaag aaagtagtgg 1141 actacacaac agccaaggaa tttgctgact ctttagcaat tcccttcctt gagacgagcg 1201 ctaagaatgc cacaaatgtg gagcaggcct tcatgactat ggctgctgag atcaagaagc 1261 gtatgggccc tggagcgaca accggaggag acaagcccaa cctgaagatc gagagcactc 1321 ctgtgcggca gtctggcgga ggctgctgtt gaacagcttg agcgtgccaa ctttattata 1381 caaagttggc attataaaaa agcattgctt atcaatttgt tgcaacgaac aggtcactat 1441 cagtcaaaat aaaatcatta tttggagctc catggtagcg ttaacgcggc cgcgatatcc 1501 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc 1561 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt 1621 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga 1681 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata 1741 atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt 1801 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac 1861 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg 1921 atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag 1981 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc 2041 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg 2101 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg 2161 gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt 2221 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa 2281 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc 2341 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg 2401 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct 2461 aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg 2521 gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag 2581 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 2641 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 2701 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc 2761 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 2821 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 2881 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 2941 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 3001 attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 3061 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 3121 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 3181 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 3241 ggccttttgc tcacatgtt //