LOCUS Exported 3310 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Multisite gateway vector for 3' tagging with rab20. ACCESSION . VERSION . KEYWORDS p3E-rab20 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3310) TITLE Parton Lab Plasmids for in vivo cell biology in the zebrafish REFERENCE 2 (bases 1 to 3310) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3310 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind complement(624..748) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1419..1514) /gene="mutant version of attL" /label=attL3 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1551..1570) /label=T7 /note="T7 promoter, forward primer" promoter complement(1552..1570) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1575..1591) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1575..1591) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1693..1712) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1704..2513 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1771..1790) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2660..3248 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3149..3168 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac 601 taataccatc taagtagttg gttcatagtg actgcatatg ttgtgtttta cagtattatg 661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt 721 ctcgttcaac tttcttgtac aaagtggtga acgttggaaa gacttctctc ctccacagat 781 acacggagag gaagtttaag gacacgataa gcaccgtcgg aggggcgttt ttcctcaaac 841 agtggggtcc ctacaacatt tccatatggg atactgcagg tcgagagcag tttcacggtc 901 tgggctccat gtactgccgt ggcgcagctg ctgtcatcct aacatatgat gtcaccaact 961 ggcagagcct ggcggagctg gagaaccgct tcctgtcctt gactgacact gccaacgcag 1021 actgcatctt cgcccttgtg gggaacaaag cggacctgac cgacccgttc gctcaagagc 1081 ctgacagcga gagtgggagc gacccgcagc ctgtagcctc ctgcccgact cctcctgcca 1141 gccctgtcca gcacaaacag gtgagcagag aggacgctgt ggctctgtac gcccgcgttc 1201 tccgatacaa aggcctggac gagaagacct gcctgcccgc cgagaggatg tgcttcgaga 1261 ccagcgctaa aacaggttac aacgtggacg ctttgttcga gaccttattt gatttagtac 1321 tgccatccat tctgaagaag aggagcgagg gtgaagccac tgtagatctg gagacgccac 1381 tagaggtgag aaggactaaa gctggctgct gtgcatagca actttattat acaaagttgg 1441 cattataaaa aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa 1501 taaaatcatt atttggagct ccatggtagc gttaacgcgg ccgcgatatc ccctatagtg 1561 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct 1621 gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca 1681 taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat 1741 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc 1801 aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga 1861 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc 1921 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat 1981 ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg 2041 attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc 2101 ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac 2161 gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg 2221 ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca 2281 ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta 2341 ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact 2401 gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata 2461 atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat 2521 tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct 2581 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag 2641 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 2701 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 2761 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 2821 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 2881 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 2941 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 3001 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa 3061 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 3121 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 3181 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 3241 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 3301 ctcacatgtt //