LOCUS Exported 3277 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Multisite gateway vector for 3' tagging with rab2a. ACCESSION . VERSION . KEYWORDS p3E-rab2a SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3277) TITLE Parton Lab Plasmids for in vivo cell biology in the zebrafish REFERENCE 2 (bases 1 to 3277) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3277 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind complement(624..748) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1386..1481) /gene="mutant version of attL" /label=attL3 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1518..1537) /label=T7 /note="T7 promoter, forward primer" promoter complement(1519..1537) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1542..1558) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1542..1558) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1660..1679) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1671..2480 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1738..1757) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2627..3215 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3116..3135 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac 601 taataccatc taagtagttg gttcatagtg actgcatatg ttgtgtttta cagtattatg 661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt 721 ctcgttcaac tttcttgtac aaagtggtgg cgtacgcgta tctcttcaag tacatcatca 781 tcggagacac gggtgtggga aagtcgtgcc tattattaca gttcacagat aagcgctttc 841 agccggtgca cgatctcact attggtgttg aatttggagc gcgaatgatc actatagatg 901 gcaaacagat aaaacttcag atctgggata cggctggaca ggagtccttc cgctcaatca 961 ccaggtctta ctacagaggt gcagctggag ctctgctagt ttatgacatc acaagaagag 1021 acacttttaa tcacttgaca acctggttag aagatgctcg tcaacattcc aactctaaca 1081 tggtcatcat gctcatcgga aataaaagtg atttggagtc acggagagag gtgaagaaag 1141 aggaaggaga ggcctttgcc agagaacatg gcttgatctt catggagact tctgcaaaga 1201 ccgcatcaaa cgtagaagag gcatttatca acacagctaa ggagatttat gaaaagatcc 1261 aggagggagt ctttgatata aacaacgagg ctaacggcat taagattgga ccccagcatg 1321 ctgccaccaa ctccacgatg ggcggcagtc agggaggaca gcaagccgga gggggctgtt 1381 gctgacaact ttattataca aagttggcat tataaaaaag cattgcttat caatttgttg 1441 caacgaacag gtcactatca gtcaaaataa aatcattatt tggagctcca tggtagcgtt 1501 aacgcggccg cgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc 1561 agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat 1621 catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata 1681 ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt 1741 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga 1801 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta 1861 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc 1921 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag 1981 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag 2041 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg 2101 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt 2161 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt 2221 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt 2281 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat 2341 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac 2401 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga 2461 tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta 2521 cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt 2581 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 2641 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 2701 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 2761 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 2821 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 2881 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 2941 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 3001 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 3061 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 3121 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 3181 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 3241 ggttcctggc cttttgctgg ccttttgctc acatgtt //