LOCUS Exported 3274 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Multisite gateway vector for 3' tagging with rab32b. ACCESSION . VERSION . KEYWORDS p3E-rab32b SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3274) TITLE Parton Lab Plasmids for in vivo cell biology in the zebrafish REFERENCE 2 (bases 1 to 3274) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3274 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind complement(624..748) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1383..1478) /gene="mutant version of attL" /label=attL3 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1515..1534) /label=T7 /note="T7 promoter, forward primer" promoter complement(1516..1534) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1539..1555) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1539..1555) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1657..1676) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1668..2477 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1735..1754) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2624..3212 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3113..3132 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac 601 taataccatc taagtagttg gttcatagtg actgcatatg ttgtgtttta cagtattatg 661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt 721 ctcgttcaac tttcttgtac aaagtggtga ggaaaatgtc aggagactca gagttaggat 781 gcactgaaca tttatttaaa gttttggtga ttggagacca taaagttggg aagagcagct 841 ttgttaaact ctatgtgagg aatcagtttt acgaggagtt gaaaaccagt ataggtgtgg 901 atttttctat gaaggttatc gattgggacg ctcacacacg agtgagattg cagttttggg 961 atatagctgg tcaagagcgt gtgcgaggac tgaatcgtgt gtacttcagg ggacccagtg 1021 gtgcttttgt agtatacgac ctgacaagcg gctcatcgct ggatggggct ctgaactgga 1081 aacgtgaact cgattgtcaa gtaatgttga aaaatggcca tccgatccct gctgttctgc 1141 tggctaataa atgtgatgag actaaagtga gccggaggaa cgcatccctt ctggagaagc 1201 tctgccagga gaagggtttt actgggtgct ttcagacttc agcaaaggag aatatcaatg 1261 tggatgaagc tgctaaattt cttgtgaagc acattctcca aattaacgaa gaatcaatga 1321 aagaagaaga gcagaagatt cacctcaaac cacacacaaa cacaaactcg gtctgctgct 1381 gacaacttta ttatacaaag ttggcattat aaaaaagcat tgcttatcaa tttgttgcaa 1441 cgaacaggtc actatcagtc aaaataaaat cattatttgg agctccatgg tagcgttaac 1501 gcggccgcga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc 1561 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat 1621 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc 1681 aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata 1741 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc 1801 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag 1861 atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt 1921 ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat 1981 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt 2041 tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat 2101 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg 2161 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc 2221 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg 2281 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc 2341 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc 2401 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc 2461 tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag agcattacgc 2521 tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt 2581 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 2641 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 2701 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 2761 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 2821 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 2881 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 2941 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 3001 acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 3061 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 3121 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 3181 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 3241 tcctggcctt ttgctggcct tttgctcaca tgtt //