LOCUS Exported 3314 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Multisite gateway vector for 3' tagging with rab3ab. ACCESSION . VERSION . KEYWORDS p3E-rab3ab SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3314) TITLE Parton Lab Plasmids for in vivo cell biology in the zebrafish REFERENCE 2 (bases 1 to 3314) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3314 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind complement(624..748) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1423..1518) /gene="mutant version of attL" /label=attL3 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1555..1574) /label=T7 /note="T7 promoter, forward primer" promoter complement(1556..1574) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1579..1595) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1579..1595) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1697..1716) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1708..2517 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1775..1794) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2664..3252 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3153..3172 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac 601 taataccatc taagtagttg gttcatagtg actgcatatg ttgtgtttta cagtattatg 661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt 721 ctcgttcaac tttcttgtac aaagtggtgg cttcagctac agacaaccgt tatggacaaa 781 aggagtcttc tgaccagaac tttgattaca tgttcaaaat cctaatcatc gggaatagca 841 gcgttgggaa gaccagcttt ctgtttcgct atgcagatga ctccttcacg ccagcgttcg 901 taagcaccgt cggcattgac ttcaaggtga agaccatcta caggaacgac aagaggataa 961 agctgcaaat ctgggacaca gcggggcagg agcgttacag aaccatcacc accgcttatt 1021 accgtggagc catgggattc atcctcatgt acgacattac caatgaggag tctttcgctg 1081 cagtgcagga ctggtccaca cagattaaga catactcatg ggataacgct caggtgcttc 1141 tagtaggcaa caagtgtgac atggaggatg agagggtcgt ggcttctgaa aggggcagac 1201 agctctctga gcatcttggt tttgaatatt tcgaagccag tgccaaggac aacattaatg 1261 ttaaacaaac cttcgagcgg ttggtggaca taatctgcga gaagatgtca gagagcctgg 1321 atgctgccga tcctgcagtg acgggagcca aacaagggcc gcagctcaca gagcagcccg 1381 ccgccccgca ccgggactgc gcctgctgaa aatcccacag ctcaacttta ttatacaaag 1441 ttggcattat aaaaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc 1501 aaaataaaat cattatttgg agctccatgg tagcgttaac gcggccgcga tatcccctat 1561 agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat 1621 ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct 1681 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg 1741 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc 1801 gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt 1861 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac 1921 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat 1981 gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat 2041 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg 2101 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa 2161 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg 2221 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc 2281 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt 2341 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg 2401 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt 2461 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca 2521 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca 2581 agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc 2641 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 2701 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 2761 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg 2821 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 2881 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 2941 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 3001 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga 3061 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 3121 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 3181 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 3241 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 3301 tttgctcaca tgtt //