LOCUS Exported 3502 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Multisite gateway vector for 3' tagging with rab40b. ACCESSION . VERSION . KEYWORDS p3E-rab40b SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3502) TITLE Parton Lab Plasmids for in vivo cell biology in the zebrafish REFERENCE 2 (bases 1 to 3502) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3502 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind complement(624..748) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1611..1706) /gene="mutant version of attL" /label=attL3 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1743..1762) /label=T7 /note="T7 promoter, forward primer" promoter complement(1744..1762) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1767..1783) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1767..1783) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1885..1904) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1896..2705 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1963..1982) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2852..3440 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3341..3360 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac 601 taataccatc taagtagttg gttcatagtg actgcatatg ttgtgtttta cagtattatg 661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt 721 ctcgttcaac tttcttgtac aaagtggtgg gtcatcgggc tgatgtgaag atgaaccaca 781 ggagcagccc agcaaaagct tatgattttc ttctgaaatt cctactggtt ggagacagtg 841 atgtggggaa aggggaaata ctggccagtc ttcaggatgg gtccagtgag tcaccctatg 901 gatacaacat gggaatcgac tacaagacta ctaccattct cctggatgga cgcagggtta 961 agcttcaact gtgggacacc tcaggacaag gcagattctg taccatattc cgctcctatt 1021 ccagaggagc acagggagtc atccttgttt atgacatcac aaaccggtgg tctttcgacg 1081 gcatcgacag atggattaaa gagattgatg agcatgcacc aggtgtcccc aagatcctgg 1141 tgggcaacag gcttcatctg gcctataagc gtcaggtgac caccgaacat gcgcaggcct 1201 ttgcagagcg attgggcgtc acgttttttg aggtcagccc tctctgtaac ttcaacatca 1261 ccgagtcctt cactgaactg gcccgcattg tgctcatgag gcacggcatg gagcgactct 1321 ggagacccaa taaagtgttg agtctgcagg acctgtgctg ccgttctatc gtttcctgca 1381 ctccagtgca tctcgtggac aaacttcccc ttcctgttgc cttaaagagt catctgaagt 1441 ccttttccat ggctaacggc ctggtcaaca ccaggatgat gcacggccgt tcctattcgg 1501 tcatacctag cagtgctaaa aaacggaacg gaaccaaaaa atccaaaatc atctatcctc 1561 cactgagccc atcccagcac tgcgcacgga caagctgcaa gatttcatag caactttatt 1621 atacaaagtt ggcattataa aaaagcattg cttatcaatt tgttgcaacg aacaggtcac 1681 tatcagtcaa aataaaatca ttatttggag ctccatggta gcgttaacgc ggccgcgata 1741 tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt 1801 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac 1861 tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt 1921 cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg 1981 ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag 2041 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca 2101 gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc 2161 ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc caggtattag 2221 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt 2281 tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc 2341 aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta 2401 atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg 2461 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat 2521 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca 2581 tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat 2641 atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt 2701 tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg acttgacggg 2761 acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt 2821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 2881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 2941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 3001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 3061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 3121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 3181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 3241 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 3301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 3361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 3421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 3481 gctggccttt tgctcacatg tt //