LOCUS Exported 3304 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Multisite gateway vector for 3' tagging with constitutively active rab5c. ACCESSION . VERSION . KEYWORDS p3E-rab5c-CA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3304) TITLE Parton Lab Plasmids for in vivo cell biology in the zebrafish REFERENCE 2 (bases 1 to 3304) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3304 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind complement(624..748) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1413..1508) /gene="mutant version of attL" /label=attL3 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1545..1564) /label=T7 /note="T7 promoter, forward primer" promoter complement(1546..1564) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1569..1585) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1569..1585) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1687..1706) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1698..2507 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1765..1784) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2654..3242 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3143..3162 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac 601 taataccatc taagtagttg gttcatagtg actgcatatg ttgtgtttta cagtattatg 661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt 721 ctcgttcaac tttcttgtac aaagtggtgg cggggcgagg tggaccagcg cggaccaacg 781 gtgccgcccc cgtcgggaac aaaatctgcc agtttaagct tgtgttgctg ggagagtctg 841 cagtaggcaa gtccagcctg gtgctgcgct tcgtcaaagg ccagtttcac gagtaccagg 901 agagcaccat tggagctgct ttcctcacac agacactctg cttggacgat acgacagtaa 961 agtttgagat ttgggacacg gccggactgg agcggtatca cagtttggcc cctatgtatt 1021 acagaggagc ccaggcagcc atcgtcgtct acgacattac taacacagac acattcacta 1081 gagcaaagaa ctgggtgaag gagctgcagc gccaggccag tccaaatatc gttattgctc 1141 tggcaggcaa caaagcagat ctggccaaca agagagccgt tgactttcag gaggcccagg 1201 catacgcaga tgacaacagc ctactcttca tggagacgtc tgcaaagacc gccatgaacg 1261 tcaatgagat cttcatggct atcgccaaga agcttccgaa aagcgagccg cagggtggag 1321 cgggcagcgg gggacgggcc cgaggagggg tggacttaca ggagacggcg cctcagggaa 1381 gaagcggcca gtgctgtgga ggcggaaact aacaacttta ttatacaaag ttggcattat 1441 aaaaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat 1501 cattatttgg agctccatgg tagcgttaac gcggccgcga tatcccctat agtgagtcgt 1561 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt 1621 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca 1681 gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt 1741 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag 1801 gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg 1861 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg 1921 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac 1981 tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag 2041 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt 2101 gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga 2161 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac 2221 aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg 2281 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg 2341 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg 2401 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg 2461 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta 2521 attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac 2581 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga 2641 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 2701 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 2761 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt 2821 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 2881 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 2941 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 3001 tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca 3061 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 3121 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 3181 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 3241 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 3301 tgtt //