LOCUS Exported 3481 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Multisite gateway vector for 3' tagging with Rab19. ACCESSION . VERSION . KEYWORDS p3E-Rab19 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3481) TITLE Parton Lab Plasmids for in vivo cell biology in the zebrafish REFERENCE 2 (bases 1 to 3481) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3481 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind complement(624..748) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1590..1685) /gene="mutant version of attL" /label=attL3 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1722..1741) /label=T7 /note="T7 promoter, forward primer" promoter complement(1723..1741) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1746..1762) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1746..1762) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1864..1883) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1875..2684 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1942..1961) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2831..3419 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3320..3339 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac 601 taataccatc taagtagttg attcatagtg actgcatatg ttgtgtttta cagtattatg 661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt 721 ctcgttcaac tttcttgtac aaagtggtgc tgttctaccg agcaaaggtc acctttcaag 781 acagcactgt ttgtgctcga tttcactcgt actttaaaaa ctgtttatct acagcagaca 841 gggaaagact ggggttttct cctagcatta gctttgtgtg tgtttgtgtt tgtgcagacg 901 gagatgcagt ggtgcaggac gtctcttttc caggtataca gtccgcaggg cgagaggacg 961 aggagcgctg tgattttctg ttcaagatca tcctgattgg ggacagtaat gtggggaaga 1021 cctgtgttat ccagagcttc aggtcagcag aggtcactga actgcagcac aacactattg 1081 gagtggattt taccgtgcgc agcatggatg ttgacggcag gagagtcaag atgcaggtgt 1141 gggacacggc gggacaggag cgtttccgca ccatcactca gagctattat cgcagtgctc 1201 atggcgctat gattgcgtat gatctgagcc gccgcgacac cttcgactct ctgccacact 1261 ggattcaggc gctggagcag tacggagctg ccagcctcgt cctcgtactc atcggtaata 1321 agtgtgatct ggaggcgcag cggcaggtgc tgttcgagga cgcctgtacg ctagcggagc 1381 gcagtggtgc tctggccgct ttagaaacat ccgccaaaca ccatcacaac atcgaggagg 1441 ctttccaact gatggccaga gaactcttag tgcgacacgg aggaatacat tatcaggaca 1501 accaatcaga ttcacccacc gtctacctgc actctgactc acacccaatc gaaggagaac 1561 agttggagaa aaggtcatgt gactgctaac aactttatta tacaaagttg gcattataaa 1621 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat 1681 tatttggagc tccatggtag cgttaacgcg gccgcgatat cccctatagt gagtcgtatt 1741 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca 1801 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta 1861 atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca 1921 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg 1981 cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca 2041 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat 2101 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca 2161 ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg 2221 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta 2281 attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata 2341 acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag 2401 tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg 2461 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg 2521 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg 2581 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata 2641 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt 2701 ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa 2761 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca 2821 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 2881 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 2941 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag 3001 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 3061 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 3121 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 3181 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc 3241 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 3301 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 3361 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 3421 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 3481 t //