LOCUS       Exported                 660 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAG414GPD-ccdB-HA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 660)
  TITLE     S cerevisiae Advanced Gateway Destination Vectors
REFERENCE   2  (bases 1 to 660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(41..165)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(173..189)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(173..189)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(278..301)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 agcataaagt gtaaagcctg ggggtgccta atgcggccgc catagtgact ggatatgttg
       61 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt
      121 tatatcattt tacgtttctc gttcagcttt tttgtacaaa cttgtgatgg gggatccact
      181 agttctagaa tccgtcgaaa ctaagttctg gtgttttaaa actaaaaaaa agactaacta
      241 taaaagtaga atttaagaag tttaagaaat agatttacag aattacaatc aatacctacc
      301 gtctttatat acttattagt caagtagggg aataatttca gggaactggt ttcaaccttt
      361 tttttcagct ttttccaaat cagagagagc agaaggtaat agaaggtgta agaaaatgag
      421 atagatacat gcgtgggtca attgccttgt gtcatcattt actccaggca ggttgcatca
      481 ctccattgag gttgtgcccg ttttttgcct gtttgtgccc ctgttctctg tagttgcgct
      541 aagagaatgg acctatgaac tgatggttgg tgaagaaaac aatattttgg tgctgggatt
      601 cttttttttt ctggatgcca gcttaaaaag cgggctccat tatatttagt ggatgccagg
//
