LOCUS       Exported                 641 bp ds-DNA     linear   SYN 10-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAG415GPD-ccdB-HA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 641)
  TITLE     S cerevisiae Advanced Gateway Destination Vectors
REFERENCE   2  (bases 1 to 641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 10, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(114..238)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             279..368
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      387..634
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(403..421)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
       61 ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg
      121 actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
      181 atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggtga
      241 tgggctgcag gaattcgata tcaagcttag gtggaatgta cccatacgat gttcctgact
      301 atgcgggcta tccctatgac gtcccggact atgcaggatc ctatccatat gacgttccag
      361 attacgctgc tcagtgctaa ctcgagtcat gtaattagtt atgtcacgct tacattcacg
      421 ccctcccccc acatccgctc taaccgaaaa ggaaggagtt agacaacctg aagtctaggt
      481 ccctatttat ttttttatag ttatgttagt attaagaacg ttatttatat ttccaatttt
      541 tctttttttt ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga
      601 aaaggttttg ggacgctcga aggctttaat ttgcggccgg t
//
