LOCUS       Exported                 645 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAG416GPD-ccdB-HA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 645)
  TITLE     S cerevisiae Advanced Gateway Destination Vectors
REFERENCE   2  (bases 1 to 645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(29..153)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(161..177)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(161..177)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(266..289)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 aaagcctggg ggtgcctaat gcggccgcca tagtgactgg atatgttgtg ttttacagta
       61 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta
      121 cgtttctcgt tcagcttttt tgtacaaact tgtgatgggg gatccactag ttctagaatc
      181 cgtcgaaact aagttctggt gttttaaaac taaaaaaaag actaactata aaagtagaat
      241 ttaagaagtt taagaaatag atttacagaa ttacaatcaa tacctaccgt ctttatatac
      301 ttattagtca agtaggggaa taatttcagg gaactggttt caaccttttt tttcagcttt
      361 ttccaaatca gagagagcag aaggtaatag aaggtgtaag aaaatgagat agatacatgc
      421 gtgggtcaat tgccttgtgt catcatttac tccaggcagg ttgcatcact ccattgaggt
      481 tgtgcccgtt ttttgcctgt ttgtgcccct gttctctgta gttgcgctaa gagaatggac
      541 ctatgaactg atggttggtg aagaaaacaa tattttggtg ctgggattct ttttttttct
      601 ggatgccagc ttaaaaagcg ggctccatta tatttagtgg atgcc
//
