LOCUS Exported 4053 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Cloning Module. ACCESSION . VERSION . KEYWORDS pUG-65 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4053) AUTHORS Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M TITLE UbiGate: a synthetic biology toolbox to analyse ubiquitination. JOURNAL New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 2017 Nov 30. PUBMED 29194629 REFERENCE 2 (bases 1 to 4053) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4053 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 30..47 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(1967..1985) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 2053..2157 /gene="bla" /label=AmpR promoter CDS 2379..3170 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 3341..3929 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3830..3849 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc 61 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatca ctctgtggtc 121 tcaaatgatg gctgaagccg gtgacgagaa tctttacgcc gccgccagag acatcgccag 181 agctctcggc aaggatccaa gtgccgccgg agatatctta cagattctct ccggttatgg 241 cgcttccgga aaccgcggcg gagatcctcg acccacccca tcccgcggcg gctcaaacgt 301 caactttgac cgagccctca cctctttaga gagacagatc tccagctaca tcgtggaaga 361 tcgaccgatc tggtctgatc ccgttgactc gcgcaccttc ctcgactctg ttgatgagct 421 acttgcgatt gccggagact tgagatccat ggccggagat aaatccgtcg ccgtctgtca 481 atcccgtgcc gatgagctga ttcaacaggt gatgtttagg cttcaagaag agtttggatt 541 tgtaatggac cgagcccctg actcgtttga ctcggacgat gagttccccg gcgaagagga 601 caacgacaca agcgacgggg taatcgttgc tcgtccaatc actgattaca aaatcgtgat 661 cgaggcgtta caatctagcg ttattggcga tctaaacgcg atcgctgtgc gcatggtcgc 721 tggtggattt gcaaaagagt gttcaagagt gtacagcagt cgacgaagag agtttctaga 781 ggagagtttg tcgaggttac atctcagagg actaagtatg gaggaagtgc aagagagtcc 841 atggcaagat cttgaggacg aaatcgaccg ttggattaaa gccgtaacgc tgatattcca 901 cgttttcttc ccgagcgagc ggcttctctg cgatcgcgtt ttctctgatc ttcctgtttc 961 ctctgtaacc gatctttctt tcatggaagt ttgtcgcgga accacaacac agcttctgaa 1021 tttcgcagac gcgatcgctt taggaagccg tttacctgaa cgtttgttta aggttgtgga 1081 tttatacgaa gcgatgcaag accttattcc taagatggaa acgctatttt ctgatcgata 1141 ttgttcgccg ctaaggcatg aggcgctcgc gattcataaa cggttaggag aagcgattag 1201 agggatattt atggagctgg agaatttaat ccgacgtgac cctcccaaaa cagcgtttcc 1261 tggcggtggg attcacccga tcacgcggta cgtgatgaat tatctgcgtg cggcgtgtaa 1321 atcgcggcaa tcgttggagc agatacttga ccagacagga aatgaaaccg gatcagatac 1381 cagaccgttg tctgtgcaaa tcatatgggt tttggagttg ttggagagta acttggaagg 1441 caagaaaaga acttatcgtg atccgtcttt gtgtttcttg ttcatgatga acaatgacaa 1501 gtatattctt gataaggcta aggataacga gctaggttta gtcctaggag aagattggat 1561 tgtgaagcac gcagcgaaac tgaggcaata ccattctaat taccgaagga gctcatggaa 1621 ccaagtggtg ggattgctta ggaccgatgg tccgtatccc aagttagtag agaacctaag 1681 attgttcaaa tcacagttcg atgaggtgtg taaggtgcaa tctcaatggg ttgttagtga 1741 tgggcaattg agggaggagt taagaagctc tgttgccgga attgtatctc cggcgtactc 1801 gaatttcatc agaagattga aggagtcacc ggagattaat gggaggcgtg gagagccgtt 1861 tattccgtat actgttgaag atgtggagtt tataatcaaa cggttgttca aggaaagctc 1921 aagttgagct ttgagaccac gaagtggctc ttcagtggac gaaagggcct cgtgatacgc 1981 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt 2041 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 2101 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg 2161 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg 2221 gttttcatgg cttgttatga ctgttttttt ttggggtaca gtctatgcct cgggcatcca 2281 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta 2341 cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gagggaagcg gtgatcgccg 2401 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt 2461 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagcgata 2521 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca 2581 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag 2641 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc 2701 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg 2761 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc 2821 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg 2881 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc 2941 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg 3001 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta 3061 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag 3121 aatttgtcca ttacgtaaaa ggcgagatca ccaaggtagt cggcaaataa ctgtcagacc 3181 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 3241 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 3301 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 3361 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 3421 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 3481 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 3541 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 3601 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 3661 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 3721 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 3781 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 3841 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 3901 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 3961 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 4021 ataaccgtat taccgccttt gagtgagctg ata //