LOCUS Exported 2295 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Synthetic biology. ACCESSION . VERSION . KEYWORDS pJOG140 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2295) AUTHORS Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J TITLE Peripheral infrastructure vectors and an extended set of plant parts for the Modular Cloning system. JOURNAL PLoS One. 2018 May 30;13(5):e0197185. doi: 10.1371/journal.pone.0197185. eCollection 2018. PUBMED 29847550 REFERENCE 2 (bases 1 to 2295) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2295 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 32..51 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 285..302 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(464..482) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 550..654 /gene="bla" /label=AmpR promoter CDS 876..1667 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 1838..131 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 61 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 121 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 181 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 241 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 301 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 361 aatcactctg tggtctcacc atggcttctc ttgctctcaa acttgcaggt cttgatatta 421 gcgcaatgtg agaccacgaa gtggctcttc agtggacgaa agggcctcgt gatacgccta 481 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg 541 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 601 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgcgc 661 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt 721 ttcatggctt gttatgactg tttttttttg gggtacagtc tatgcctcgg gcatccaagc 781 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc 841 agcagggcag tcgccctaaa acaaagttaa acatcatgag ggaagcggtg atcgccgaag 901 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc 961 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agcgatattg 1021 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg 1081 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca 1141 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat 1201 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca 1261 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag 1321 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa 1381 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta 1441 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg 1501 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac 1561 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat 1621 ttgtccatta cgtaaaaggc gagatcacca aggtagtcgg caaataactg tcagaccaag 1681 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 1741 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 1801 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 1861 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 1921 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 1981 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 2041 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 2101 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 2161 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 2221 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 2281 taagcggcag ggtcg //