LOCUS Exported 3042 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Synthetic biology. ACCESSION . VERSION . KEYWORDS pAGM16429 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3042) AUTHORS Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J TITLE Peripheral infrastructure vectors and an extended set of plant parts for the Modular Cloning system. JOURNAL PLoS One. 2018 May 30;13(5):e0197185. doi: 10.1371/journal.pone.0197185. eCollection 2018. PUBMED 29847550 REFERENCE 2 (bases 1 to 3042) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3042 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 16..120 /gene="bla" /label=AmpR promoter CDS 342..1133 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 1304..1892 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1793..1812 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2046..2063 /label=L4440 /note="L4440 vector, forward primer" CDS 2151..2168 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 2211..2927 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITHGMDELYK" primer_bind complement(2256..2277) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(2517..2536) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2864..2885 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(2972..2990) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg 61 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt 121 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg 181 gcggttttca tggcttgtta tgactgtttt tttttggggt acagtctatg cctcgggcat 241 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg 301 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgagggaa gcggtgatcg 361 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga 421 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagcg 481 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga 541 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag 601 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac 661 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga 721 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag 781 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa 841 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag 901 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg 961 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag 1021 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg 1081 aagaatttgt ccattacgta aaaggcgaga tcaccaaggt agtcggcaaa taactgtcag 1141 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 1201 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 1261 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 1321 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 1381 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 1441 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 1501 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 1561 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 1621 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 1681 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 1741 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 1801 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 1861 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 1921 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 1981 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 2041 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc 2101 ccgcgcgttg gccgattcat taatcactct gtggtctcac catgggcagc catcaccacc 2161 atcatcacag cggactagca ctgaagcttg ctggcctcga catcaacggg atggtgagca 2221 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa 2281 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga 2341 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca 2401 ccttcagcta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact 2461 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg 2521 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca 2581 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt 2641 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg 2701 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc 2761 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca 2821 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt 2881 tcgtgaccgc cgccgggatc actcacggca tggacgagct gtacaagggt tcaatgtgag 2941 accacgaagt ggctcttcag tggacgaaag ggcctcgtga tacgcctatt tttataggtt 3001 aatgtcatga taataatggt ttcttagacg tcaggtggca ct //