LOCUS Exported 2471 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Synthetic biology. ACCESSION . VERSION . KEYWORDS pJOG317 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2471) AUTHORS Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J TITLE Peripheral infrastructure vectors and an extended set of plant parts for the Modular Cloning system. JOURNAL PLoS One. 2018 May 30;13(5):e0197185. doi: 10.1371/journal.pone.0197185. eCollection 2018. PUBMED 29847550 REFERENCE 2 (bases 1 to 2471) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2471 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 4..23 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 257..274 /label=L4440 /note="L4440 vector, forward primer" CDS 355..444 /codon_start=1 /product="3 tandem Myc epitope tags" /label=3xMyc /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL" CDS 385..474 /codon_start=1 /product="3 tandem Myc epitope tags" /label=3xMyc /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL" CDS 481..570 /codon_start=1 /product="two Strep-Tag(R)II moieties connected by a linker for enhanced binding to Strep-Tactin(R), an engineered form of streptavidin (Schmidt et al., 2013)" /label=Twin-Strep-tag /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK" primer_bind complement(612..630) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 698..802 /gene="bla" /label=AmpR promoter CDS 1024..1815 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 1986..103 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 61 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 121 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 181 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag 241 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa 301 accgcctctc cccgcgcgtt ggccgattca ttaatcactc tgtggtctca ttcggaacaa 361 aagttgatct ctgaagagga ccttgagcag aaattgatct ctgaggaaga tcttgagcag 421 aagcttatct cagaggagga tttggagcaa aaacttattt ctgaagagga ccttgccgct 481 tctgcttggt cacacccaca gttcgagaag ggaggtggat ctggtggagg ttcaggagga 541 tcagcttggt cacatcctca atttgaaaaa tagctttgag accacgaagt ggctcttcag 601 tggacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt 661 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 721 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 781 ataatattga aaaaggaaga gtatgcgctc acgcaactgg tccagaacct tgaccgaacg 841 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttttggg 901 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg 961 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac 1021 atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc 1081 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat 1141 ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt aaggcttgat 1201 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag 1261 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg 1321 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca 1381 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga 1441 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa 1501 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg 1561 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc 1621 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag 1681 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg 1741 gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga gatcaccaag 1801 gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 1861 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 1921 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 1981 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 2041 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 2101 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac 2161 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 2221 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 2281 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 2341 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 2401 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 2461 agggagcttc c //