LOCUS Exported 3363 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Cloning Module. ACCESSION . VERSION . KEYWORDS pUG-419 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3363) AUTHORS Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M TITLE UbiGate: a synthetic biology toolbox to analyse ubiquitination. JOURNAL New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 2017 Nov 30. PUBMED 29194629 REFERENCE 2 (bases 1 to 3363) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3363 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 90..107 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(1337..1355) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 1423..1527 /gene="bla" /label=AmpR promoter CDS 1749..2540 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 2711..3299 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3200..3219 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 61 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc 121 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatca ctctgtggtc 181 tcaaatgaac accggcggtg gccaatacac ggattttccg gcggtggaaa ctcacggagg 241 acagttcata agttacgata tcttcggtag tttattcgag atcacatcta agtatcgtcc 301 tccgattata ccaattggtc gtggagctta tggaatcgtt tgctctgtgt tggatacgga 361 gacgaacgag ctagtagcga tgaagaagat agctaatgct tttgataatc atatggatgc 421 taagcgtacg cttcgtgaga tcaagcttct tcgtcatctt gatcatgaaa acattatagc 481 tataagagat gttgttccac caccactaag aaggcagttc agtgatgttt atatctctac 541 tgaattaatg gatactgatc ttcatcaaat catcagatct aaccagagtt tatcagaaga 601 acactgtcag tacttcttgt accagctact tcgaggactg aagtatatcc actcagctaa 661 cattattcat agggatttaa agccgagcaa tcttctgttg aacgcgaatt gcgatttaaa 721 gatttgtgat ttcggtcttg ctagacctac ttcagagaat gattttatga ctgagtatgt 781 tgttacgaga tggtatagag cacctgagct tctgttgaac tcttcagatt acacagctgc 841 tattgatgtt tggtctgttg gttgtatctt tatggagctt atgaatagaa agcctttgtt 901 ccctggtaaa gaccatgttc atcaaatgcg cttattgaca gagttgcttg gcacaccgac 961 agaatctgat ctcggtttta ctcacaatga ggatgcgaaa agatacatcc ggcaacttcc 1021 caacttccca cgtcagccct tagctaaact tttctctcat gttaacccaa tggccattga 1081 tcttgttgac agaatgttga cgtttgaccc caacagaaga atcactgttg aacaagctct 1141 gaatcaccag taccttgcta aattgcacga cccgaatgat gagccaatct gtcaaaagcc 1201 attctctttt gagttcgaac aacagcctct ggatgaggaa cagataaaag agatgatcta 1261 ccaagaagcc atagcactca atccaacata cggttaggct ttgagaccac gaagtggctc 1321 ttcagtggac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata 1381 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 1441 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 1501 cttcaataat attgaaaaag gaagagtatg cgctcacgca actggtccag aaccttgacc 1561 gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt 1621 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat 1681 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt 1741 taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg 1801 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag 1861 tggatggcgg cctgaagcca cacagcgata ttgatttgct ggttacggtg accgtaaggc 1921 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg 1981 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc 2041 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc 2101 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag 2161 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc 2221 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg 2281 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag 2341 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg 2401 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc 2461 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ttacgtaaaa ggcgagatca 2521 ccaaggtagt cggcaaataa ctgtcagacc aagtttactc atatatactt tagattgatt 2581 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 2641 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 2701 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 2761 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 2821 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag 2881 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 2941 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 3001 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 3061 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 3121 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 3181 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 3241 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 3301 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 3361 tct //