LOCUS Exported 8511 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses Flag-tagged human URH49 (DDX39A). ACCESSION . VERSION . KEYWORDS Hy_pMT Flag-URH49 WT SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8511) AUTHORS Huang C, Liang D, Tatomer DC, Wilusz JE TITLE A length-dependent evolutionarily conserved pathway controls nuclear export of circular RNAs. JOURNAL Genes Dev. 2018 May 17. pii: gad.314856.118. doi: 10.1101/gad.314856.118. PUBMED 29773557 REFERENCE 2 (bases 1 to 8511) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8511 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 621..640 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(859..963) /gene="bla" /label=AmpR promoter primer_bind 1031..1049 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 2445..2724 /label=copia promoter /note="strong promoter from the Drosophila transposable element copia (Sinclair et al., 1986)" CDS 2770..3795 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E" intron 4158..4223 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 4353..4373 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 4798..4932 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" promoter 4933..5359 /label=MT promoter /note="Drosophila metallothionein promoter" CDS 5378..5443 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" polyA_signal 6748..6869 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7579..7596) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7750..8338) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7830..7849) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8509..858) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" ORIGIN 1 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 61 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 121 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 181 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 241 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 301 tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 361 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 421 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 481 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 541 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 601 ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat 661 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 721 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 781 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 841 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 901 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 961 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt 1021 aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattca catttgtacg 1081 aatttttttt ttatcaaaag ttcgagtttt tcaccaattt cctcatcaac cgagcaaggc 1141 aaacggcttt gaataatatg gtgttatata tacatatatc aaatcgctgc tgactgcgtg 1201 attgatggcc ccaagattac atattatcga atcaggattc agaaggagat caatgtcaaa 1261 tgcggacagg aacatgaaag acgcctgtta tgcgcaatta aaaatttggg tttaattgct 1321 gtggaaactg ttgttggcgg catcttaagt tcctgtttaa caacatcaac tacttatgta 1381 cgtagaagcg tttaagccat ttgcatacag atgagaactg gcttttgtgc taatcagtca 1441 agatgactcc gatgatgatg actcattacc tgaccagttt tcgctgcttt cttttcaaca 1501 actacttgta tatgtattgt atccaatagc aatacattga atttccatgg tctagtcacg 1561 tattatcatt taattgacac caagtcgtgt tattgttgag ctatcgagtt cagctcaaac 1621 atttcttatt cccatgaata agccggcaaa aatatgcaat ctatgaaagt taatataagc 1681 aaaccttact ttgactcaat accaatgcac tttgtgtcga taggttcacg caattgaggc 1741 gattattccg ataacccaag cgattgactg ttcccgtttc gattccaatt gaaatttgga 1801 aatgtacaat agttttgcta tatgctgtca agtacgctct tatcttctct gggttttctt 1861 cagagtttcg aaacgcttct tctttttttt gttttttttt ttttggaatc tcgtattttg 1921 gaaggggctc ccctctggaa tttgttacac tgtcgttatc attgcgaaca agcggcccga 1981 agctatcagc gactttaaca tttacaatgc acttttttac gaccaattaa atgtacattt 2041 tcctttcttc gcccgttgat aagcgaacgc gatgtggcgc aggcaatgtg ttgctcttgc 2101 gacacaaacg caatcaaaat ggattcaatt tcgctttttc ccagtgaaac gaagaacgaa 2161 ccgaccatca tgatatgctc ctctgcatgt tgcgtattga atcaatgaca atttcaatta 2221 agccgcccgt tcgtcatgcg ttttcgtgcg cttcgaaatg ctgataacgc tgctgtcctc 2281 caactgcttt gcatgtggac acaattccat ttatttaatt cttttatttg gatcggttaa 2341 attaaaaagc gccttgttac gcatttaacg ttgtttccgg tgcgtggtgg tttcatgctt 2401 ctgggaacgg caaatgggtt taggattggg aacccctcat catctgttgg aatatactat 2461 tcaacctaca aaagtaacgt taaacaacac tactttatat ttgatatgaa tggccacacc 2521 ttttatgcca taaaacatat tgtaagagaa taccactctt tttattcctt ctttccttct 2581 tgtacgtttt ttgctgtaag taggtcgtgg tgctggtgtt gcagttgaaa taacttaaaa 2641 tataaatcat aaaactcaaa cataaacttg actatttatt tatttattaa gaaaggaaat 2701 ataaattata aattacaaca ggttatggac ctgcagccaa gcttggcgct cgtccggggg 2761 caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 2821 aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 2881 agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 2941 tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 3001 cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt 3061 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag 3121 gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 3181 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 3241 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 3301 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 3361 gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 3421 agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 3481 tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 3541 ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 3601 ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 3661 cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 3721 accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 3781 agggcaaagg aatagagtag atgccgaccg aacaagagct gatttcgaga acgcctcagc 3841 cagcaactcg cgcgagccta gcaaggcaaa tgcgagagaa cggccttacg cttggtggca 3901 cagttctcgt ccacagttcg ctaagctcgc tcggctgggt cgcgggaggg ccggtcgcag 3961 tgattcaggc ccttctggat tgtgttggtc cccagggcac gattgtcatg cccacgcact 4021 cgggtgatct gactgatccc gcagattgga gatcgccgcc cgtgcctgcc gattgggtgc 4081 agatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag 4141 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga 4201 ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt 4261 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg 4321 aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc 4381 ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa 4441 ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa 4501 ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat cataacatac 4561 tgttttttct tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat 4621 tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg 4681 ccttgactag agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa 4741 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac 4801 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 4861 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 4921 catgtctgga tcaattcgtt gcaggacagg atgtggtgcc cgatgtgact agctctttgc 4981 tgcaggccgt cctatcctct ggttccgata agagacccag aactccggcc ccccaccgcc 5041 caccgccacc cccatacata tgtggtacgc aagtaagagt gcctgcgcat gccccatgtg 5101 ccccaccaag agctttgcat cccatacaag tccccaaagt ggagaaccga accaattctt 5161 cgcgggcaga acaaaagctt ctgcacacgt ctccactcga atttggagcc ggccggcgtg 5221 tgcaaaagag gtgaatcgaa cgaaagaccc gtgtgtaaag ccgcgtttcc aaaatgtata 5281 aaaccgagag catctggcca atgtgcatca gttgtggtca gcagcaaaat caagtgaatc 5341 atctcagtgc aactaaaggg gggatctcga gaccatggac tacaaagacc atgacggtga 5401 ttataaagat catgacatcg attacaagga tgacgatgac aagaccggtg gtaccatggc 5461 agaacaggat gtggaaaacg atcttttgga ttacgatgaa gaggaagagc cccaggctcc 5521 tcaagagagc acaccagctc cccctaagaa agacatcaag ggatcctacg tttccatcca 5581 cagctctggc ttccgggact ttctgctgaa gccggagctc ctgcgggcca tcgtggactg 5641 tggctttgag catccttctg aggtccagca tgagtgcatt ccccaggcca tcctgggcat 5701 ggacgtcctg tgccaggcca agtccgggat gggcaagaca gcggtcttcg tgctggccac 5761 cctacagcag attgagcctg tcaacggaca ggtgacggtc ctggtcatgt gccacacgag 5821 ggagctggcc ttccagatca gcaaggaata tgagcgcttt tccaagtaca tgcccagcgt 5881 caaggtgtct gtgttcttcg gtggtctctc catcaagaag gatgaagaag tgttgaagaa 5941 gaactgtccc catgtcgtgg tggggacccc gggccgcatc ctggcgctcg tgcggaatag 6001 gagcttcagc ctaaagaatg tgaagcactt tgtgctggac gagtgtgaca agatgctgga 6061 gcagctggac atgcggcggg atgtgcagga gatcttccgc ctgacaccac acgagaagca 6121 gtgcatgatg ttcagcgcca ccctgagcaa ggacatccgg cctgtgtgca ggaagttcat 6181 gcaggatccc atggaggtgt ttgtggacga cgagaccaag ctcacgctgc acggcctgca 6241 gcagtactac gtcaaactca aagacagtga gaagaaccgc aagctctttg atctcttgga 6301 tgtgctggag tttaaccagg tgataatctt cgtcaagtca gtgcagcgct gcatggccct 6361 ggcccagctc ctcgtggagc agaacttccc ggccatcgcc atccaccggg gcatggccca 6421 ggaggagcgc ctgtcacgct atcagcagtt caaggatttc cagcggcgga tcctggtggc 6481 caccaatctg tttggccggg ggatggacat cgagcgagtc aacatcgtct ttaactacga 6541 catgcctgag gactcggaca cctacctgca ccgggtggcc cgggcgggtc gctttggcac 6601 caaaggccta gccatcactt ttgtgtctga cgagaatgat gccaaaatcc tcaatgacgt 6661 ccaggaccgg tttgaagtta atgtggcaga acttccagag gaaatcgaca tctccacata 6721 catcgagcag agccggtaag cggccgcaac ttgtttattg cagcttataa tggttacaaa 6781 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 6841 ggtttgtcca aactcatcaa tgtatcttaa ctagtggatc cactaggggc cgccgacgcg 6901 aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc 6961 gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc 7021 gctcgcggct cttaccagcc taacttcgat cattggaccg ctgatcgtca cggcgattta 7081 tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg ccctatacct 7141 tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga cctgaatgga 7201 agccggcggc acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg 7261 cggagaactg tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc 7321 tccagcagcc gcacgcggcg catctcgggc agcgttgggt cctggccacg ggtgcgcatg 7381 atcgtgctcc tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag 7441 aatgaatcac cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc 7501 tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag 7561 tcagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 7621 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 7681 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 7741 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 7801 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 7861 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 7921 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 7981 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 8041 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 8101 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 8161 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 8221 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 8281 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 8341 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 8401 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 8461 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt a //