LOCUS Exported 6951 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Express Chrimson and mCherry in mechanosensory neurons.. ACCESSION . VERSION . KEYWORDS pmec-4::Chrimson::SL2::mCherry::unc-54 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6951) AUTHORS Liu M, Sharma AK, Shaevitz JW, Leifer AM TITLE Temporal processing and context dependency in Caenorhabditis elegans response to mechanosensation. JOURNAL Elife. 2018 Jun 26;7. pii: 36419. doi: 10.7554/eLife.36419. PUBMED 29943731 REFERENCE 2 (bases 1 to 6951) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6951 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 397..416 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(635..739) /gene="bla" /label=AmpR promoter rep_origin complement(765..1220) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(902..923) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 1114..1133 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 1347..1369 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 1361..1378 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 1362..1378 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 1388..1407 /label=T7 /note="T7 promoter, forward primer" promoter 1388..1406 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 2470..3519 /codon_start=1 /product="red light-drivable channelrhodopsin from Chlamydomonas noctigama (Klapoetke et al., 2014)" /label=Chrimson /translation="MAELISSATRSLFAAGGINPWPNPYHHEDMGCGGMTPTGECFSTE WWCDPSYGLSDAGYGYCFVEATGGYLVVGVEKKQAWLHSRGTPGEKIGAQVCQWIAFSI AIALLTFYGFSAWKATCGWEEVYVCCVEVLFVTLEIFKEFSSPATVYLSTGNHAYCLRY FEWLLSCPVILIKLSNLSGLKNDYSKRTMGLIVSCVGMIVFGMAAGLATDWLKWLLYIV SCIYGGYMYFQAAKCYVEANHSVPKGHCRMVVKLMAYAYFASWGSYPILWAVGPEGLLK LSPYANSIGHSICDIIAKEFWTFLAHHLRIKIHEHILIHGDIRKTTKMEIGGEEVEVEE FVEEEDEDTV" primer_bind complement(5525..5545) /label=T3 /note="T3 promoter, forward primer" promoter complement(5525..5543) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(5564..5580) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5564..5580) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(5577..5599) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 5588..5604 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(5612..5642) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 5657..5678 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(5795..5812) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5966..6554) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6046..6065) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(6725..634) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" ORIGIN 1 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 61 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 121 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 181 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 241 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 301 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 361 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 421 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 481 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 541 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 601 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 661 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 721 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa 781 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc 841 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt 901 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa 961 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg 1021 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg 1081 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc 1141 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa 1201 tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg 1261 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 1321 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga 1381 gcgcgcgtaa tacgactcac tatagggcga attggagctc caccgcggac tagtggatcc 1441 cccgggctgc agaagcttca atacaagctc aaatacaata ctattttttt tccgtctgaa 1501 cataaaatta gtttctaaat aaaaaatgtg aaaataaata tttggtttta atcgttaaaa 1561 tcttccaaat tactaataaa gttcagtcaa ctatatgctt cgaaattcac tgaatctatt 1621 attgttatta atttttagac ccttggagag ctcccaactc tttcaatcca gaagactgat 1681 ggatgccagg tgtacttgtc aaaggtggct caaggatgcg agattgtcac ttccaaatcc 1741 agtgagatga acatctccgt gcaaaccaat gatgatgggg attatgtaag tttttcggat 1801 tgtagaaccc aatctaaatt tccttttcag tcggagttcc cggttccgga gcagttcaag 1861 acaacttttg tcaatggcaa gctagtcact gtcgtctcgg acatttgcta aatcttctca 1921 cgtcataacc ttatttttca acttcatgaa ctatcccctc gaactttttt atttgtccct 1981 gtctacaatt ctttataagc aattgagaca cactttgctt tattaaataa taccgatttt 2041 gttttgtata tgcattgact ggtgctcaac tatttttatt gaaaaaatta ctttttactg 2101 tctcgtcgaa taaaagtgat cccttccaaa aaatagttat tcatgtttga aggtttgtga 2161 tcgtagaaca attgcaccga atgtagtgta aaaaaaattc cagaatgttc aaatacattt 2221 ccaccctcac cagaacatct caaacaccag gcacatttca acacactttc atggatctta 2281 tcttgcctca aaaccatcag ctttcttctc attctacaag tatgaaacaa tctactattc 2341 tatacattta taatgtaatg gaaccatgtg aagacatgag caagatcatt gtcgtcagcg 2401 actacccctc cttcattttt cttagagtat cagtttgcta atgctatttt ttttatcgct 2461 atcaagttaa tggctgagct tatttcatct gcaacacgat cattgttcgc tgcaggagga 2521 attaatccat ggccaaaccc atatcatcac gaagatatgg gatgcggagg aatgacacca 2581 actggagagt gtttctctac tgaatggtgg tgcgatccat catatggatt gtctgatgct 2641 ggatatggat actgttttgt tgaggcaaca ggaggatact tggttgttgg agttgaaaag 2701 aaacaagcat ggctccattc acgtggaact ccaggagaaa aaattggagc tcaagtttgc 2761 caatggattg cattctcaat cgctattgca cttttgacat tctatggatt ttctgcttgg 2821 aaagcaactt gtggatggga agaggtttac gtttgttgcg ttgaggttct tttcgttaca 2881 ttggagatct tcaaggaatt ttcatctcca gctacagttt atctctcaac tggaaatcat 2941 gcatattgtc ttcgttactt tgaatggctc ctttcttgcc cagttatcct catcaaactt 3001 tcaaacttgt ctggactcaa gaacgattac tcaaagagaa caatgggact tattgtttct 3061 tgcgttggaa tgattgtttt cggaatggct gcaggattgg ctactgattg gctcaagtgg 3121 ttgctctata ttgtttcatg tatctacgga ggatacatgt actttcaagc tgcaaagtgc 3181 tatgttgaag caaatcattc tgttccaaaa ggacactgta gaatggttgt taagttgatg 3241 gcttatgcat acttcgcttc atggggatct tatccaattc tttgggcagt tggaccagaa 3301 ggacttttga aattgtcacc atacgcaaac tcaatcggac attctatctg tgatatcatc 3361 gctaaagagt tctggacatt tctcgcacat caccttagaa tcaagatcca tgaacacatc 3421 cttattcacg gagatattcg aaaaacaact aagatggaga ttggaggaga agaggttgaa 3481 gttgaagagt ttgttgaaga ggaagatgaa gatactgttg gtaccgctgt ctcatcctac 3541 tttcacctag ttaactgctt gtcttaaaat ctatgcttct ctttagtatc taaaattttc 3601 ctagaagctt acaagtatat aaatggtctc ttctcaataa aggttgtata tttattcatc 3661 ttattgaatc tgccatttcc tcgtttttgc gagtttatat accttccaat tttctttcta 3721 ttgtattttc aacttctaat tttaattcag ggaaactgct tcaacgcatc atggtctcaa 3781 agggtgaaga agataacatg gcaattatta aagagtttat gcgtttcaag gtgcatatgg 3841 agggatctgt caatgggcat gagtttgaaa ttgaaggtga aggagaaggc cgaccatatg 3901 agggaacaca aaccgcaaaa ctaaaggtaa gtttaaacat atatatacta actaaccctg 3961 attatttaaa ttttcaggta actaaaggcg gaccattacc attcgcctgg gacatcctct 4021 ctccacagtt catgtatgga agtaaagctt atgttaaaca tccggcagat ataccagatt 4081 atttgaaact ttcattcccg gagggtttta agtgggaacg cgtaatgaat tttgaagacg 4141 gaggagttgt tacagtgacg caagactcaa ggtaagttta aacagttcgg tactaactaa 4201 ccatacatat ttaaattttc agcctccaag atggagaatt tatttataaa gtcaaacttc 4261 gaggaacgaa tttcccctcg gatggacctg ttatgcagaa gaagactatg ggatgggaag 4321 cttcaagtga aagaatgtac cctgaagacg gtgctcttaa gggagagatt aaacaacgtc 4381 ttaaattgaa agatggagga cattacgatg ctgaggtaag tttaaacatg attttactaa 4441 ctaactaatc tgatttaaat tttcaggtga agacaactta caaagccaaa aaaccagttc 4501 agctgccagg agcgtacaat gttaatatta aactggatat cacctcccac aacgaggatt 4561 acactatcgt tgagcaatat gaaagagctg aagggcggca ctcgacaggt ggcatggatg 4621 aattgtataa ggcgccggtc gctaccatta ccaacttgtc tggtgtcaaa aataataggg 4681 gccgctgtca tcagagtaag tttaaactga gttctactaa ctaacgagta atatttaaat 4741 tttcagcatc tcgcgcccgt gcctctgact tctaagtcca attactcttc aacatcccta 4801 catgctcttt ctccctgtgc tcccaccccc tatttttgtt attatcaaaa aacttctctt 4861 aatttctttg ttttttagct tcttttaagt cacctctaac aatgaaattg tgtagattca 4921 aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg tgctccctcc ccccattaat 4981 aataattcta tcccaaaatc tacacaatgt tctgtgtaca cttcttatgt tttttacttc 5041 tgataaattt ttttgaaaca tcatagaaaa aaccgcacac aaaatacctt atcatatgtt 5101 acgtttcagt ttatgaccgc aatttttatt tcttcgcacg tctgggcctc tcatgacgtc 5161 aaatcatgct catcgtgaaa aagttttgga gtatttttgg aatttttcaa tcaagtgaaa 5221 gtttatgaaa ttaattttcc tgcttttgct ttttggggtt tcccctattg tttgtcaaga 5281 tttcgaggac ggcgtttttc ttgctaaaat cacaagtatt gatgagcacg atgcaagaaa 5341 gatcggaaga aggtttgggt ttgaggctca gtggaaggtg agtagaagtt gataatttga 5401 aagtggagta gtgtctatgg ggtttttgcc ttaaatgaca gaatacattc ccaatatacc 5461 aaacataact gtttcctact agtcggccgt acgggccctt gggcccggta cccagctttt 5521 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg 5581 tgtgaaattg ttatccgctc acaattccac acaacatacg agccgggagc ataaagtgta 5641 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 5701 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 5761 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 5821 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 5881 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 5941 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 6001 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 6061 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 6121 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 6181 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 6241 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 6301 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 6361 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta 6421 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 6481 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 6541 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 6601 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 6661 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 6721 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgccta tttcgttcat 6781 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 6841 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 6901 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta t //