LOCUS       Exported                4912 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  cGAL-N driver under the control of C. elegans rab-3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pHW530 (Prab-3::cGAL-N::let-858 3'UTR)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Wang H, Liu J, Yuet KP, Hill AJ, Sternberg PW
  TITLE     Split cGAL, an intersectional strategy using a split intein for 
            refined spatiotemporal transgene control in Caenorhabditiselegans.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Mar 26. pii: 1720063115. doi: 
            10.1073/pnas.1720063115.
  PUBMED    29581308
REFERENCE   2  (bases 1 to 4912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        2621..2725
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2726..3586
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2944..2963)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3757..4345
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4246..4265
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4499..4516
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4633..4654
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4669..4699
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4707..4723
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4712..4734
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4731..4747
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4731..4747
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gatcttcaga tgggagcagt ggactgtcta tttgatattc ttaaaatgta ctctataaaa
       61 gatgaaactg taatgaccaa ttccctacat aaatattttt aactaattgg tcggatattt
      121 ggggatcaga gaagtacttg tttttgcgat attaataatg atatattttt ctagtaaaaa
      181 aatgttaagc gatttgtgaa taattaagtt ggcttgaaaa agattttttg atagctatgt
      241 attttgttaa tttaaatcag attttaaaac tttattattg actatttata tatattatat
      301 attatatatt ttcatggttt aatcaaaatc tattaaatta tttctaaaat ctctaaatat
      361 cgaattctct aaaaagcttc atcaacaaaa gcatttcaat tatctaacac aatgagaata
      421 attcaagaac tgaaatagaa agaaatctag aaaaaaaaga gagtgaggtg ccctattaaa
      481 tcggtgctct cctctctaac agaagcgcct acacgcaccc aagagagctc tttttctttc
      541 ttttttcgga gcacacatac acgcatacac tcctcagaag tgtatagaga aaatgggaga
      601 agaagcgaaa gaagacgaag aagcggttgt gatgtatgca tcgggcgttt ctatctctct
      661 ccgtgagcaa cgagctagtc aacccatcga tccatttttg tgagcacaca cagagagaga
      721 ctcaaacaca ctcacagctt cacactgagc tccaagagct ctgtagagag aagccagagt
      781 atgctttcct cgattttcct aacgtcttct tcttctgctt ttttcttttt cccaacgagt
      841 tgtagtccac taacttcaat ttttcctcca gagaaagagt tttagagaga cagctgataa
      901 atacactaga gaaataaaaa gaatgataaa aatttgctat tttaatcagt tccctctcgt
      961 ttctctaagt tttgttgttg ggttgggaaa ttaaaattgg aaaatggaaa taacgttttt
     1021 ttctgtctaa tcttgaattc tccagacatt tcttatttcc ttttttgttg caagtttttc
     1081 ttccttctct aatttttaat caaagatatc tcgctatgaa ctttgaattt ttgatatttt
     1141 gattctataa atacataaag taatctaaat gctcttttaa aataaatcta cagtagccct
     1201 attttcaggg cgcgcctcta gaggatcccc gggattggcc aaaggaccca aaggtatgtt
     1261 tcgaatgata ctaacataac atagaacatt ttcaggagga cccttggcta ggatgggccc
     1321 taaaaagaag cgtaaagcta gcatgaagct gttgtcttca atggaacaag cttgcgacat
     1381 atgccggtta aaaaaactca ggtgctcgaa ggaaaagccc aagtgtgcca agtgtctgaa
     1441 gaacaactcg gagtgccgtt attctcccaa ggctatgagg tccccactga ccagggcgca
     1501 tctgacagag gtagaatcaa ggttggaaaa attggaagat ttgtttctgc tcatgtttcc
     1561 tcgggaaaac ctggacagca ttttgaacat ggactctttg gacgaagtga aagtaatgtt
     1621 gaaacaatta tatatgcagg actatggtga cacggatgct gctatggata gcttgacccc
     1681 tatggaaaat ggcatgtctg aagggccgag aaaacacatg aagactgcga catcatcgcc
     1741 aaaagtggat gaagatggaa gccaaagtca gttgagtgta tcgccggaat tcccgctgca
     1801 gtctggtggc ggaggggctc ctaggtgcct cgacctcaag acccaagtcc aaaccccaca
     1861 aggaatgaag gagatctcca acatccaagt cggagacctc gtcctctcca acaccggata
     1921 caacgaggtc ctcaacgtct tcccaaagtc caagaagaag tcctacaaga tcaccctcga
     1981 ggacggaaag gagatcatct gctccgagga gcacctcttc ccaacccaaa ccggagagat
     2041 gaacatctcc ggaggactca aggagggaat gtgcctctac gtcaaggagt aaggtaccga
     2101 attcgctagc cggccataca agtaatccgg atgatcgacg ccaacgtcgt tgaattttca
     2161 aattttaaat actgaatatt tgtttttttt cctattattt atttattctc tttgtgtttt
     2221 ttttcttgct ttctaaaaaa ttaattcaat ccaaatctaa acattttttt ttctctttcc
     2281 gtctcccaat tcgtattccg ctcctctcat ctgaacacaa tgtgcaagtt tatttatctt
     2341 ctcgctttca tttcattagg acgtgggggg aattggtgga agggggaaac acacaaaagg
     2401 atgatggaaa tgaaataagg acacacaata tgcaacaaca ttcaattcag aaatatggag
     2461 gaaggtttaa aagaaaacat aaaaatatat agaggaggaa ggaaaactag taaaaaataa
     2521 gcaaagaaat taggcgaacg atgagaattg tcctcgcttg ggcccaaaag gccttttttt
     2581 tttggcgccg acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     2641 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     2701 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     2761 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     2821 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     2881 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     2941 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     3001 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     3061 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     3121 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     3181 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     3241 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     3301 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     3361 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     3421 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     3481 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     3541 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     3601 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     3661 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     3721 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3781 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3841 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3901 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3961 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4021 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4081 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4141 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4201 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4261 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4321 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4381 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     4441 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     4501 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     4561 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     4621 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     4681 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     4741 ctatgaccat gattacgcca agctgtaagt ttaaacatga tcttactaac taactattct
     4801 catttaaatt ttcagagctt aaaaatggct gaaatcactc acaacgatgg atacgctaac
     4861 aacttggaaa tgaaataagc ttgcatgcgc ggccgcactg actgggccgg cc
//
