LOCUS Exported 2892 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Bacterial expression of mutant human alpha synuclein. ACCESSION . VERSION . KEYWORDS pT7-7 asyn S129W SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2892) TITLE Lashuel Lab plasmids REFERENCE 2 (bases 1 to 2892) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2892 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 14..602 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 503..522 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 756..773 /label=L4440 /note="L4440 vector, forward primer" primer_bind 1008..1030 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 1095..1114 /label=T7 /note="T7 promoter, forward primer" promoter 1095..1113 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" RBS 1145..1167 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS complement(1726..2586) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2349..2368 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2587..2691) /gene="bla" /label=AmpR promoter primer_bind 2759..2777 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 61 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 121 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt 181 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 241 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 301 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 361 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 421 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 481 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 541 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 601 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 661 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag 721 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg 781 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatag 841 gaagatcttc cggaagatct tcctatggtg cactctcagt acaatctgct ctgatgcgct 901 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg 961 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat 1021 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cccagcgatt cgaacttctg 1081 atagacttcg aaattaatac gactcactat agggagacca caacggtttc cctctagaaa 1141 taattttgtt taactttaag aaggagatat acatatggat gtattcatga aaggactttc 1201 aaaggccaag gagggagttg tggctgctgc tgagaaaacc aaacagggtg tggcagaagc 1261 agcaggaaag acaaaagagg gtgttctcta tgtaggctcc aaaaccaagg agggagtggt 1321 gcatggtgtg gcaacagtgg ctgagaagac caaagagcaa gtgacaaatg ttggaggagc 1381 agtggtgacg ggtgtgacag cagtagccca gaagacagtg gagggagcag ggagcattgc 1441 agcagccact ggctttgtca aaaaggacca gttgggcaag aatgaagaag gagccccaca 1501 ggaaggaatt ctggaagata tgcctgtgga tcctgacaat gaggcttatg aaatgccttg 1561 ggaggaaggg tatcaagact acgaacctga agcctaagaa atatctttgc tcccagtttc 1621 ttgagatctg ctgacagatg ttccatccaa gcttatcgat gataagctgt caaacatgag 1681 aattaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 1741 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 1801 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 1861 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 1921 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 1981 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 2041 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 2101 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 2161 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 2221 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 2281 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 2341 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 2401 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 2461 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 2521 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 2581 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 2641 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 2701 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 2761 taggcgtatc acgaggccct ttcgtcttca agaataaaag gatctaggtg aagatccttt 2821 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 2881 ccgtagaaaa ga //