LOCUS       Exported                2892 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Bacterial expression of mutant human alpha synuclein.
ACCESSION   .
VERSION     .
KEYWORDS    pT7-7 asyn G51D
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Fares MB, Ait-Bouziad N, Dikiy I, Mbefo MK, Jovicic A, Kiely A, 
            Holton JL, Lee SJ, Gitler AD, Eliezer D, Lashuel HA
  TITLE     The novel Parkinson's disease linked mutation G51D attenuates in 
            vitro aggregation and membrane binding of alpha-synuclein, and 
            enhances its secretion and nuclear localization in cells.
  JOURNAL   Hum Mol Genet. 2014 Sep 1;23(17):4491-509. doi: 10.1093/hmg/ddu165. 
            Epub 2014 Apr 11.
  PUBMED    24728187
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      15..603
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     504..523
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     757..774
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1009..1031
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1096..1115
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1096..1114
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1146..1168
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             complement(1727..2587)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2350..2369
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2588..2692)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2760..2778
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
       61 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
      121 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
      181 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
      241 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
      301 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
      361 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
      421 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
      481 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
      541 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
      601 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
      661 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
      721 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
      781 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
      841 ggaagatctt ccggaagatc ttcctatggt gcactctcag tacaatctgc tctgatgcgc
      901 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
      961 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     1021 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcccagcgat tcgaacttct
     1081 gatagacttc gaaattaata cgactcacta tagggagacc acaacggttt ccctctagaa
     1141 ataattttgt ttaactttaa gaaggagata tacatatgga tgtattcatg aaaggacttt
     1201 caaaggccaa ggagggagtt gtggctgctg ctgagaaaac caaacagggt gtggcagaag
     1261 cagcaggaaa gacaaaagag ggtgttctct atgtaggctc caaaaccaag gagggagtgg
     1321 tgcatgatgt ggcaacagtg gctgagaaga ccaaagagca agtgacaaat gttggaggag
     1381 cagtggtgac gggtgtgaca gcagtagccc agaagacagt ggagggagca gggagcattg
     1441 cagcagccac tggctttgtc aaaaaggacc agttgggcaa gaatgaagaa ggagccccac
     1501 aggaaggaat tctggaagat atgcctgtgg atcctgacaa tgaggcttat gaaatgcctt
     1561 ctgaggaagg gtatcaagac tacgaacctg aagcctaaga aatatctttg ctcccagttt
     1621 cttgagatct gctgacagat gttccatcca agcttatcga tgataagctg tcaaacatga
     1681 gaattaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     1741 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     1801 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     1861 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     1921 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     1981 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     2041 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     2101 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     2161 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     2221 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     2281 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     2341 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     2401 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     2461 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     2521 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     2581 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     2641 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     2701 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     2761 ataggcgtat cacgaggccc tttcgtcttc aagaataaaa ggatctaggt gaagatcctt
     2821 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     2881 cccgtagaaa ag
//
